| Project/Area Number |
22390189
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Keiji 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00196809)
MITSUTAKE Norisato 長崎大学, 大学院・医歯薬学総合研究科, 助教 (50404215)
SAENKO Vladimir 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (30343346)
大津留 晶 長崎大学, 長崎大学病院, 准教授 (00233198)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | 内分泌学 / 甲状腺がん / 遺伝子多型解析 / 細胞機能解析 / がん基礎研究 |
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| Research Abstract |
We have successfully established a cell culture system from primary human thyroid tissues and identified the multi-potential stem cell-like cells in a specific serum-free medium condition. One speculates that there is a profound relationship of reprogramming and mesenchymal transition between stem cell-like cells and differentiated cells from thyroid tissues. These findings may suggest a new aspect and relationship of thyroid cancer stem cells during its carcinogenesis. Furthermore we have studied the functional role of FOXE1 that is a major thyroid cancer-associated SNP gene. Using immunohistochemistry, specific patterns of FOXE1 expression in thyroid carcinomas have been identified. We have separately clarified a strong association between the SNPs of FOXE1 and NKX-2, and the Japanese thyroid cancer cases. This is the first report on the different ethnics besides Caucasians. Finally we found the new evidence of imanitib, a molecular targeting agent, selectively inhibited an increase of NFkappa B activity induced by irradiation in cultured thyroid cancer cell lines. The combination chemotherapy with radiation may become more efficient therapeutic approach for a poor-prognosis thyroid anaplastic cancer patient clinically.
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