Experimental research of immunological tolerance in allograft from cadaveric donors for clinical trial

• Project/Area Number: 22390246
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: FUKUSHIMA Norihide 大阪大学, 医学系研究科, 寄附講座教授 (30263247)
• Co-Investigator(Kenkyū-buntansha): 高原 史郎 大阪大学, 医学系研究科, 寄附講座教授 (70179547) ; 矢澤 浩治 大阪大学, 医学系研究科, 助教 (40569109)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 移植外科学 / アカゲザル / 死体腎臓移植 / 免疫寛容 / 骨髄移植（髄内） / サイモグロブリン / キメラ / MLR / CD2,CD20 / 骨髄移植(髄内移植) / リンパ球分画分析 / CD2, CD20 / 死体臓器移植(腎・心)

Research Abstract

During the study period, it was revealed in Rhesus monkeys that anti-human thymocyte globulin may reduce the number of T-cell, that chimerism of peripheral blood mononuclear cells (PMBC) can be detected and that bone marrow transplant can be done by perfusion technique. Although chimerism of PBMC was not observed, mixed lymphocyte reaction (MLR) against the donor lymphocytes was significantly reduced compared to that against lymphocytes of third party monkey