Development of an acellular cartilage tissue engineering technique using an ultra-purified in-situ forming gel

• Project/Area Number: 22390285
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Hokkaido University
• Principal Investigator: IWASAKI Norimasa 北海道大学, 大学院・医学研究科, 教授 (30322803)
• Co-Investigator(Kenkyū-buntansha): KASAHARA Yasuhiko 北海道大学, 病院・整形外科, 助教 (00581927) ; HUNAKOSHI Tadanao 北海道大学, 病院・整形外科, 講師 (10528334) ; 金城 政孝 北海道大学, 先端生命科学研究科(研究院), 教授 (70177971) ; 藤崎 和弘 独立行政法人理化学研究所, その他部局等, 研究員 (90435678) ; 三浪 明男 北海道大学, 大学院・医学研究科, 教授 (20133738)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
• Keywords: 軟骨再生 / SDF-1 / 無細胞治療 / ウサギ / 骨髄間葉幹細胞 / 骨髄間葉系細胞

Research Abstract

We succeeded in achieving hyaline-like cartilage repair by local administration of SDF-1 using an ultra-purified in-situ forming (UPIF) gel without cells. The obtained results indicate that UPIF gel enhances chondrogenesis of bone marrow stromal cells recruited by the chemotactic effect of SDF-1, leading to a novel cell-free approach for cartilage tissue engineering.

Research Products

• [Journal Article] Repair of rabbit osteochondral defects by an acellular technipue with an ultrapurified alginate gel containing stromal cell-derived factor-1 (2012)
  • Author(s): Sukegawa A, Iwasaki N, et al
  • Journal Title: Tissue Eng Part A Volume: 18 Pages: 934-945
  • Peer Reviewed
• [Journal Article] Repair of articular cartilage defects with a novel injectable in situ forming material in a canine model (2012)
  • Author(s): Igarashi T, Iwasaki N, et al
  • Journal Title: J Biomed. Mater. Res A Volume: 100 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Therapeutic effects of intra-articular ultra-purified low endotoxin alginate administration on experimental osteoarthritis in rabbits (2012)
  • Author(s): Igarashi T, Iwasaki N, et al
  • Journal Title: Cartilage Volume: 3 Pages: 69-77
  • Peer Reviewed
• [Journal Article] Repair of rabbit osteochondral defects by an acellular technique with an ultrapurified alginate gel containing stromal cell-derived factor 1 (2012)
  • Author(s): Sukegawa A, Iwasaki N, Kasahara Y, Onodera T, Igarashi T, Minami A
  • Journal Title: Tissue Engineering Part A Volume: 18 Pages: 934-945
  • Peer Reviewed
• [Journal Article] Therapeutic effects of intra-articular ultra-purified low endotoxin alginate administration on experimental osteoarthritis in rabbits. (2012)
  • Author(s): Igarashi T, Iwasaki N , Kawamura T, Tsukuda Y, Kasahara Y, Todoh M, Tadano S, Minami A
  • Journal Title: Cartilage Volume: 3 Pages: 69-77
  • Peer Reviewed
• [Journal Article] Repair of articular cartilage defects with a novel injectable in situ forming material in a canine model. (2012)
  • Author(s): Igarashi T, Iwasaki N , Kawamura T, Kasahara Y, Tsukuda Y, Ohzawa N, Ito M, Izumisawa Y, Minami A
  • Journal Title: J Biomed. Mater. Res A. Volume: 100 Issue: 1 Pages: 180-187
  • DOI: 10.1002/jbm.a.33248
  • Peer Reviewed
• [Journal Article] Repair of rabbit osteochondral defects by an acellular technique with an ultrapurified alginate gel containing stromal cell-derived factor-1. (2012)
  • Author(s): Sukegawa A, Iwasaki N , Kasahara Y, Onodera T, Igarashi T, Minami A:
  • Journal Title: Tissue Eng Part A. Volume: 18 Issue: 9-10 Pages: 934-945
  • DOI: 10.1089/ten.tea.2011.0380
  • Peer Reviewed
• [Journal Article] Transplantation of tissue-engineered cartilage for the treatment of osteochondritis dissecans in the elbow. -outcomes over a 4-year follow-up in 2 cases (2010)
  • Author(s): Iwasaki N, et al
  • Journal Title: J Shoulder Elbow Surg Volume: 16
  • Peer Reviewed
• [Journal Article] A Cellular Implantation System Using an Injectable Ultra-purified Alginate Gel for Repair of Osteochondral Defects in a Rabbit Model (2010)
  • Author(s): Igarashi T, Iwasaki N, et al
  • Journal Title: J Biomed. Mater. Res. A Volume: 94 Pages: 844-855
  • Peer Reviewed
• [Journal Article] Autologous osteochondral mosaicplasty for osteochondritis dissecans of the elbow in teenage athletes (2010)
  • Author(s): Iwasaki, N, et al
  • Journal Title: J Bone Joint Surg Volume: 92 Pages: 208-216
  • NAID: 120001644265
  • Peer Reviewed
• [Journal Article] Autologous osteochondral mosaicplasty for osteochondritis dissecans of the elbow in teenage athletes (2010)
  • Author(s): Iwasaki, N, et al
  • Journal Title: Journal of Bone & Joint Surgery Volume: 92 Suppl 1 Pages: 208-216
  • NAID: 120001644265
  • Peer Reviewed
• [Journal Article] A Cellular Implantation System Using an Injectable Ultra-purified Alginate Gel for Repair of Osteochondral Defects in a Rabbit Model (2010)
  • Author(s): Igarashi T, Iwasaki N, et al
  • Journal Title: Journal of Biomedical Materials Research Part A Volume: 94 Pages: 844-855
  • Peer Reviewed
• [Journal Article] Transplantation of tissue-engineered cartilage for the treatment of osteochondritis dissecans in the elbow,-outcomes over a 4-year follow-up in 2 cases- (2010)
  • Author(s): Iwasaki N, et al
  • Journal Title: Journal of Shoulder and Elbow Surgery Volume: 19
  • Peer Reviewed
• [Presentation] 高純度低エンドトキシンアルギン酸ゲルの犬変形性膝関節症モデルに対する治療 (2013)
  • Author(s): 佃幸憲、岩崎倫政
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪府)
  • Year and Date: 2013-03-12
• [Presentation] 高純度低エンドトキシンアルギン酸ゲルの犬変形性膝関節症モデルに対する治療 (2013)
  • Author(s): 佃 幸憲
  • Organizer: 第２６回日本軟骨代謝学会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府）
• [Presentation] 臨床応用を目指した低侵襲軟骨再生治療法の開発 (2012)
  • Author(s): 岩崎倫政
  • Organizer: 第40回日本関節病学会
  • Place of Presentation: 鹿児島サン ロイヤルホテル(鹿児島県)
• [Presentation] Repair of rabbit osteochondral defects by an acellular technique with an ultrapurified alginate gel containing stromal cell-derived factor-1 (2012)
  • Author(s): Sukegawa A, Iwasaki N, Kasahara Y, Onodera T, Igarashi T, Minami A
  • Organizer: 2012 Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: Moscone West Convention CenterUSA
• [Presentation] 臨床応用を目指した低侵襲軟骨再生治療法の開発 (2012)
  • Author(s): 岩崎倫政
  • Organizer: 第４０回日本関節病学会
  • Place of Presentation: 鹿児島サンロイヤルホテル（鹿児島県）
  • Invited
• [Presentation] Repair of rabbit osteochondral defects by an acellular technique with an ultrapurified alginate gel containing stromal cell-derived factor-1 (2012)
  • Author(s): Sukegawa A, Iwasaki N, Kasahara Y, Onodera T, Igarashi T, Minami A
  • Organizer: 2012 Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: Moscone West Convention Center(米国)
• [Presentation] Analgesic effect of intra-articular administrations of ultra-purified alginate on arthritis in rats (2011)
  • Author(s): Tsukuda T, Iwasaki N, et al
  • Organizer: 2011Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: Long Beach Convention Center、USA
• [Presentation] Analgesic effect of intra-articular administrations of ultra-purified alginate on arthritis in rats (2011)
  • Author(s): Tsukuda T, Iwasaki N, et al
  • Organizer: 2011 Annual Meeting of Orthopaedic Research Society (ORS)
  • Place of Presentation: Long Beach Convention Center(米国)
• [Presentation] A cellular implantation system using a novel injectable material for cartilage tissue engineering (2010)
  • Author(s): Iwasaki N
  • Organizer: BIT'S3rd Annual Congress ofRegenerativeMedicine & Stem Cell
  • Place of Presentation: Everbright Convention & ExhibitionCenter、Shanghai, China
• [Presentation] 高純度アルギン酸ゲルのラット関節炎モデルに対する関節内投与による疼痛抑制効果 (2010)
  • Author(s): 佃幸憲、岩崎倫政、他
  • Organizer: 第25回日整会基礎
  • Place of Presentation: 国立京都国際会館京都
• [Presentation] Therapeutic effects of intra-articular administration of ultra-purified low endotoxin alginate on the progression of experimental osteoarthritis in rabbits (2010)
  • Author(s): Igarashi T, Iwasaki N, et al
  • Organizer: 2010 World Congress on Osteoarthritis
  • Place of Presentation: Square-Brussels Meeting Centre、Brussels, Belgium
• [Presentation] 新素材を用いた軟骨欠損治療の探求-低侵襲軟骨再生治療法の開発 (2010)
  • Author(s): 岩崎倫政
  • Organizer: 第83回日本整形外科学会学術総会
  • Place of Presentation: 東京国際フォーラム、東京
• [Presentation] 新素材を用いた軟骨欠損治療の探求-低侵襲軟骨再生治療法の開発- (2010)
  • Author(s): 岩崎倫政
  • Organizer: 第83回日本整形外科学会学術総会
  • Place of Presentation: 東京国際フォーラム(東京特別区)
• [Presentation] Therapeutic effects of intra-articular administration of ultra-purified low endotoxin alginate on the progression of experimental osteoarthritis in rabbits (2010)
  • Author(s): Igarashi T, Iwasaki N, et al
  • Organizer: 2010 World Congress on Osteoarthritis (OARSI)
  • Place of Presentation: Square-Brussels Meeting Centre(ベルギー)
• [Presentation] 高純度アルギン酸ゲルのラット関節炎モデルに対する関節内投与による疼痛抑制効果 (2010)
  • Author(s): 佃幸憲、岩崎倫政, 他
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 国立京都国際会館(京都市)
• [Presentation] A cellular implantation system using a novel injectable material for cartilage tissue engineering (2010)
  • Author(s): Iwasaki N
  • Organizer: BIT'S 3rd Annual World Congress of Regenerative Medicine & Stem Cell
  • Place of Presentation: Everbright Convention & Exhibition Center(中国)
• [Book] 関節疾患に対する新規注射用治療製材 (2012)
  • Author(s): 小野寺智洋、岩崎倫政
  • Total Pages: 5
  • Publisher: 化学工業
• [Book] 関節疾患に対する新規注射用治療製材. (2012)
  • Author(s): 小野寺智洋
  • Total Pages: 5
  • Publisher: 化学工業
• [Patent(Industrial Property Rights)] 軟骨再生用組成物 (2011)
  • Inventor(s): 岩崎倫政
  • Industrial Property Rights Holder: 北海道大学
  • Filing Date: 2011-08-21