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Neuranagenesis factor and comprehensive gene analysis for spinal cord injury

Research Project

Project/Area Number 22390287
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionUniversity of Fukui

Principal Investigator

BABA Hisatoshi  福井大学, 医学部, 教授 (00165060)

Co-Investigator(Kenkyū-buntansha) UCHIDA Kenzo  福井大学, 医学部, 准教授 (60273009)
Co-Investigator(Renkei-kenkyūsha) FURUKAWA Shoei  岐阜薬科大学, 薬学部, 教授 (90159129)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Keywords脊椎脊髄病学 / 脊髄神経再生 / 脊髄損傷 / 軸索再生 / 脊髄再生 / 網羅的遺伝子解析 / siRNA / 慢性脊髄圧迫 / マイクロアレイ / 遺伝子解析 / 神経再生因子 / メカニカルストレス
Research Abstract

The present study was designed to investigate the effect of cyclic tensile stresses on cultured spinal cord cells using the Flexercell Strain Unit and DNA microarray technology.Spinal cord cells were isolated for culture from 15-day Sprague-Dawley rat embryos. The application of cyclic tensile stress reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. Increasing either the strain or the strain rate independently was associated with significant decreases in spinal cord cell survival. There was no clear evidence of additive effects of strain level with strain rate. GO analysis identified 44 candidate genes which were significantly related to “apoptosis” and 17 genes related to “response to stimulus”. KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated by RT-PCR analysis. These data may prove useful, as the accurate knowledge of neuronal gene expression in response to cyclic tensile stress will help in the development of molecular-based therapies for spinal cord injury.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (31 results)

All 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results) Presentation (14 results)

  • [Journal Article] Blockade of interleukin-6 signaling inhibits the classic pathway and promotes an alternative pathway of macrophage activation after spinal cord injury in mice2012

    • Author(s)
      Guerrero AR, Uchida K, Nakajima H, Watanabe S, Nakamura M, Johnson WE, Baba H
    • Journal Title

      J Neuroinflammation

      Volume: 27:9:40

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transplantation of mesenchymal stem cells promotes an alternative pathway of macrophage activation and functional recovery after spinal cord injury2012

    • Author(s)
      Nakajima H, Uchida K, Guerrero AR, Watanabe S, Sugita D, Takeura N, Yoshida A, Long G, Wright KT, Johnson WE, Baba H
    • Journal Title

      J Neurotrauma

      Volume: 29 Pages: 1614-1625

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The retrograde delivery of adenovirus vector carrying the gene for brain-derived neurotrophic factor protects neurons and oligodendrocytes from apoptosis in the chronically compressed spinal cord of twy/twy mice2012

    • Author(s)
      Uchida K
    • Journal Title

      Spine

      Volume: 37 Pages: 2125-2135

    • NAID

      10031169894

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blockade of interleukin-6 signaling inhibits the classic pathway and promotes an alternative pathway of macrophage activation after spinal cord injury in mice2012

    • Author(s)
      Guerrero AR
    • Journal Title

      J Neuroinflammation

      Volume: 27 Pages: 40-40

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-mobility Group Box-1 and Its Receptors Contribute to Proinflammatory Response in the Acute Phase of Spinal Cord Injury in Rats2011

    • Author(s)
      Chen KB, Uchida K, Nakajima H, Yayama T, Hirai T, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Zhu P, Baba H
    • Journal Title

      Spine

      Volume: 36 Pages: 2122-2129

    • NAID

      10030901954

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tumor necrosis factor-alpha antagonist reduces apoptosis of neurons and oligodendroglia in rat spinal cord injury2011

    • Author(s)
      Chen KB, Uchida K, Nakajima H, Yayama T, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Baba H
    • Journal Title

      Spine

      Volume: 36 Pages: 1350-1358

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] High-mobility group boX-1 and its receptors contribute to proinflammatory response in the acute phase of spinal cord injury in rats2011

    • Author(s)
      Chen KB, Uchida K, Nakajima H, Yayama T, Hirai T, Rodriguez Guerrero A, Kobayashi S, Ma WY, Liu SY, Zhu P, Baba H
    • Journal Title

      Spine (Phila Pa 1976)

      Volume: 36 Pages: 2122-2129

    • NAID

      10030901954

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tumor necrosis factor-α antagonist reduces apoptosis of neurons and oligodendroglia in rat spinal cord injury2011

    • Author(s)
      Chen KB, Uchida K, Nakajima H, Yayama T, Hirai T, Watanabe S, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Baba H
    • Journal Title

      Spine (Phila Pa 1976)

      Volume: 36 Pages: 1350-1358

    • NAID

      10030716262

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microarray analysis of expression of cell death-associated genes in rat spinal cord cells exposed to cyclic tensile stresses in vitro2010

    • Author(s)
      Uchida K, Nakajima H, Hirai T, Yayama T, Chen KB, Kobayashi S, Roberts S, Johnson WE, Baba H.
    • Journal Title

      BMC Neurosci

      Volume: 11 Pages: 84-84

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted retrograde gene delivery of brain-derived neurotrophic factor suppresses apoptosis of neurons and oligodendroglia after spinal cord injury in rats2010

    • Author(s)
      Nakajima H, Uchida K, Yayama T, Kobayashi S, Guerrero AR, Furukawa S, Baba H
    • Journal Title

      Spine

      Volume: 35 Pages: 497-504

    • NAID

      10028062172

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microarray analysis of expression of cell death-associated genes in rat spinal cord cells exposed to cyclic tensile stresses in vitro.2010

    • Author(s)
      Uchida K, Nakajima H, Hirai T, Yayama T, Chen KB, Kobayashi S, Roberts S, Johnson WE, Baba H
    • Journal Title

      BMC Neurosci

      Volume: 11 Pages: 84-84

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeted retrograde gene delivery of brain-derived neurotrophic factor suppresses apoptosis of neurons and oligodendroglia after spinal cord injury in rats.2010

    • Author(s)
      Nakajima H, Uchida K, Yayama T, Kobayashi S, Guerrero AR, Furukawa S, Baba H.
    • Journal Title

      Spine

      Volume: 35 Pages: 497-504

    • NAID

      10028062172

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脊髄再生基礎科学の現状と近未来の展望、Myelopathyに対するアデノウィルスベクターを用いた逆行性神経栄養因子遺伝子療法による脊髄保護効果2010

    • Author(s)
      内田研造、中嶋秀明、渡邉修司、馬場久敏
    • Journal Title

      脊椎脊髄ジャーナル

      Volume: 23 Pages: 859-866

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Blockade of interleukin-6 signaling inhibits the classic pathway and promotes an alternative pathway of macrophage activation after spinal cord injury in mice

    • Author(s)
      Guerrero AR, Uchida K, Nakajima H, Watanabe S, Nakamura M, Johnson WE, Baba H
    • Journal Title

      J Neuroinflammation

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transplantation of Mesenchymal Stem Cells Promotes the Alternative Pathway of Macrophage Activation and Functional Recovery after Spinal Cord Injury

    • Author(s)
      Nakajima H, Uchida K, Rodriguez Guerrero A, Watanabe s, Sugita D, Takeura N, Yoshida A, Long G, Wright K, Johnson E, Baba H
    • Journal Title

      J Neurotrauma

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tumor necrosis factor-alpha antagonist reduces apoptosis of neurons and oligodendroglia in rat spinal cord injury.

    • Author(s)
      Chen KB, Uchida K, Nakajima H, Yayama T, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Baba H.
    • Journal Title

      Spine

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-mobility group box-1 and its receptors contribute to proinflammatory response in the acute phase of spinal cord injury in rats.

    • Author(s)
      Uchida K, Nakajima H, Yayama T, Hirai T, Guerrero AR, Koba yashi S, Ma WY, Liu SY, Zhu P, Baba H.
    • Journal Title

      Spine

      Volume: (in press)

    • NAID

      10030901954

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Blockade of interleukin-6 signaling improves the survival rate of transplanted bone marrow stromal cells after spinal cord injury2012

    • Author(s)
      Nakajima H
    • Organizer
      40th Annual Meeting of the Cervical Spine Research Society
    • Place of Presentation
      Chicago, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] マウス脊髄損傷モデルを用いた骨髄間質細胞移植後のMAPK シグナル伝達経路に関する免疫組織学的検討2012

    • Author(s)
      渡邉修司
    • Organizer
      第27回日本整形外科学会基礎学術集会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] 脊髄運動ニューロンに対するヒト骨髄間質細胞の軸索伸展メカニズム2012

    • Author(s)
      内田研造
    • Organizer
      第27回日本整形外科学会基礎学術集会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] 慢性脊髄圧迫モデル(twy/twy)を用いた骨髄由来マクロファージの極性変化に関する組織学的検討2012

    • Author(s)
      平井貴之
    • Organizer
      第41回日本脊椎脊髄病学会
    • Place of Presentation
      久留米
    • Related Report
      2012 Annual Research Report
  • [Presentation] Blockade of interleukin-6 signaling generates an alternative activating environment in the spinal cord after injury:Promoting a neuroprotective macrophage population with concurrent locomotive recovery in mice2011

    • Author(s)
      Guerrero AR, Uchida K, Nakajima H, Hirai T, Watanabe S, Baba H
    • Organizer
      39th Annual Meeting of The Cervical Spine Research Society(CSRS 2011)
    • Place of Presentation
      Arizona(USA)
    • Year and Date
      2011-12-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Blockade of interleukin-6 signaling generates an alternative activating environment in the spinal cord after injury : Promoting a neuroprotective macrophage population with concurrent locomotive recovery in mice2011

    • Author(s)
      Alexander Guerrero, K. Uchida, H.Nakajima, T.Hirai, S.Watanabe, H.Baba
    • Organizer
      39th Annual Meeting of The Cervical Spine Research Society (CSRS 2011)
    • Place of Presentation
      Arizona(USA)
    • Year and Date
      2011-12-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Microarray analysis of cell death-associated genes in rat embryo cultured spinal cord cells exposed to cyclic tensile stresses in vitro2011

    • Author(s)
      Uchida K, Nakajima H, Hirai T, Watanabe S, Guerrero AR, Baba H.
    • Organizer
      The 27th Annual Meeting of the CSRS-ES
    • Place of Presentation
      Istanbul(Turkey)
    • Year and Date
      2011-06-08
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] メカニカルストレスに対する培養脊髄ニューロンの MAPK キナーゼに関する遺伝子発現プロファイル2011

    • Author(s)
      内田研造, 中嶋秀明, 彌山峰史, 杉田大輔, 渡邉修司, 馬場久敏
    • Organizer
      第84回日本整形外科学会学術総会
    • Place of Presentation
      Web 開催
    • Year and Date
      2011-05-12
    • Related Report
      2012 Final Research Report
  • [Presentation] メカニカルストレスに対する培養脊髄ニューロンのMAPKキナーゼに関する遺伝子発現プロファイル2011

    • Author(s)
      内田研造, 中嶋秀明, 彌山峰史, 杉田大輔, 渡邉修司, 馬場久敏
    • Organizer
      第84回日本整形外科学会学術総会
    • Place of Presentation
      (Web開催)
    • Year and Date
      2011-05-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Some neuroprotective mathods for spinal cord injury2011

    • Author(s)
      Baba H, Uchida K, Nakajima H, WatanabeS, Takeura N, Yayama T
    • Organizer
      The 13rd Intedrnational Southern China Cardiology Congress
    • Place of Presentation
      Guangzhou(China)
    • Year and Date
      2011-04-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Some neuroprotective mathods for spinal cord injury2011

    • Author(s)
      H.Baba, K.Uchida, H.Nakajima, S.Watanabe, N.Takeura, T.Yayama
    • Organizer
      The 13rd Intedrnational Southern China Cardiology Congress
    • Place of Presentation
      Guangzhou (China)(招待講演)
    • Year and Date
      2011-04-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cell death-associated genes expression patterns using microarray analysis in rat cultured spinal cord cells exposed to cyclic tensile stresses in vitro2010

    • Author(s)
      Uchida K, Nakajima H, Hirai T, Yayama T, Sugita D, Kobayashi S, Baba H
    • Organizer
      7^<th>Combined Meeting of ORS
    • Place of Presentation
      Kyoto (Japan)
    • Year and Date
      2010-10-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Cell death-associated genes expression patterns using microarray analysis in rat cultured spinal cord cells exposed to cyclic tensile stresses in vitro2010

    • Author(s)
      K.Uchida, H.Nakajima, T.Hirai, T.Yayama, D.Sugita, S.Kobayashi, H.Baba
    • Organizer
      7^<th>Combined Meeting of ORS
    • Place of Presentation
      京都
    • Year and Date
      2010-10-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] マイクロアレイを用いたメカニカルストレスに対する脊髄ニューロンの遺伝子発現解析2010

    • Author(s)
      内田研造, 中嶋秀明, 彌山峰史, 杉田大輔, 渡邉修司, 馬場久敏
    • Organizer
      第39回日本脊椎脊髄病学会
    • Place of Presentation
      高知
    • Year and Date
      2010-04-23
    • Related Report
      2012 Final Research Report 2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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