|Budget Amount *help
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
In recent years, a lot of medical biomaterials have been developed and have been widely used. Metal is used as a component of biomaterials in dental treatment, because metal is easy to process and metal has stiffness, elasticity, and ductility. In dentistry, the metal is frequently used as a part of denture, an implant and crown restoration. Also, it is utilized as vascular stents and artificial joint material in medical area. However, since oral disease, inflammation and allergic dermatitis are caused by metal, the risk has been pointed out previously. Recently, patients of allergic dermatitis or inflammation, which are caused by metal are increased, because the frequency of use of metal material by improving medical and dental technology is increased, and people to put the ornaments such as necklaces and earrings are increased. However, the pathogenesis of the disease induced by the metal is not well understood.In this study, we addressed to develop new diagnostic methods for delayed-type hypersensitivity and inflammation induced by metal using the experimental animal model and we examined the molecular mechanism of development for metal allergy and inflammation.In lymphocyte stimulation test using the patient's peripheral blood for diagnostic metal allergy, we found that the sensitivity is enhanced by inhibition of CTLA-4. In this method, we may develop new method of lymphocyte stimulation test for diagnostic metal allergy. In a mouse model metal allergy, we were able to develop a new animal model ofmetal allergy which is closer to the human pathology.