| Project/Area Number |
22390372
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
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| Keywords | 神経再生 / 歯髄幹細胞 / 性状解析 / 脊髄損傷 / 低酸素脳症 / パーキンソン病 / 運動機能回復 / オリゴデンドロサイト / 神経保護効果 / 軸索伸張抑制因子 / 細胞補給効果 / 医療廃棄物 |
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| Research Abstract |
We have characterized the multi-differentiation potency, phenotypic marker expression and trascriptome profile of dentalpulp derived stem cell from adult wisdom teeth (DPSC) and exfoliated deciduous teeth (SHED). The data showed that both SHED and DPSC expressing multiple neural linage markers are able to differentiate neuron, astrocyte, oligodendrocyte, osteoblast, adipocyte and chondrocytes. Furthermore, we have optimized the protocol allowing a specific differentiation of SHED and DPSC to tyrosine hydroxylase expressing dopaminerigic neurons. In pre-clinical analysis, we have found remarkable therapeutic benefits of SHED and DPSC for treatment of spinal cord injury, hypoxic brain injury and Parkinson’s disease. In addition to their remarkable neuro-regenerative activities, we did not observe the malignant transformation of engrafted SHEDs 8 weeks after theirimplantation. SHEDs and DPSCs can be obtained from exfoliated deciduous and impacted adult wisdom teeth without adverse health effects. Thus, there are few ethical concerns regarding their clinical use. We propose that tooth-derived stem cells may be an excellent and practical cellular resource for the treatment of various CNS diseases.
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