| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Research Abstract |
Adipose derived stem cells (ADSCs) have been isolated from adipose tissue by the simple method with collagenase digestion and centrifugation. Direct implantation of freshly isolated ADSCs with minimal amount of BMP could induce ectopic bone formation. ADSCs transfected with the plasmid carrying BMP and/or Wnt genes differentiated into osteoblasts in vitro. Implantation of the gene activated matrix (GAM) consisted with atelocollagen, which constructed with BMP gene by calcium precipitation method, induced bone formation and the addition of ADSCs to the GAM accelerated the bone induction.
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