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Exploration of cold-adapted microorganisms for development of new low-temperature biotechnological processes

Research Project

Project/Area Number 22404021
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Biofunction/Bioprocess
Research InstitutionKyoto University

Principal Investigator

KURIHARA Tatsuo  京都大学, 化学研究所, 教授 (70243087)

Co-Investigator(Kenkyū-buntansha) KAWAMOTO Jun  京都大学, 化学研究所, 助教 (90511238)
Project Period (FY) 2010-04-01 – 2013-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords特殊環境微生物 / 低温菌 / 好冷性酵素 / 分子育種 / 国際研究者交流 / 国際情報交換 / オーストリア / メタル代謝 / 高度不飽和脂肪酸 / ドイツ / 生物・生体工学 / 低温適応微生物
Research Abstract

New cold-adapted microorganisms were isolated from cold environments, and biochemical and biotechnological studies on cold-adapted microorganisms were performed for development of new low-temperature bioprocesses. In particular, a genetic engineering method to modify the genome of Shewanella livingstonensis Ac10, a cold-adapted bacterium isolated from Antarctic seawater, was established for its molecular breeding. Eicosapentaenoic acid, which is beneficial to human health, was found to be produced by this strain, and the mechanism of its biosynthesis was elucidated. In addition, the strain was shown to reduce various metal ions, and a protein involved in this process was identified for metal biotechnological application of this strain.

Report

(4 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (9 results) (of which Invited: 1 results)

  • [Journal Article] Characterization of 1-acyl-sn-glycerol-3-phosphate acyltransferase from a polyunsaturated fatty acid-producing bacterium, Shewanella livingstonensis Ac102012

    • Author(s)
      Hyun-Nam CHO, Wataru KASAI, Jun KAWAMOTO, Nobuyoshi ESAKI, and Tatsuo KURIHARA
    • Journal Title

      Trace Nutrients Research

      Volume: 29 Pages: 92-99

    • NAID

      40020192173

    • URL

      http://www.jtnrs.com/sym29/19-No-P-14.pdf

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of an eicosapentaenoic acid biosynthesis protein Orf2 from a psychrotrophic bacterium, Shewanella livingstonensis Ac102012

    • Author(s)
      Chunjie GONG, Jun KAWAMOTO, Nobuyoshi ESAKI, and Tatsuo KURIHARA
    • Journal Title

      Trace Nutrients Research

      Volume: 29 Pages: 84-91

    • NAID

      40020192148

    • URL

      http://www.jtnrs.com/sym29/18-No-P-13.pdf

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] dentification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis.2012

    • Author(s)
      Park, J., Kawamoto, J., Esaki, N., and Kurihara, T.
    • Journal Title

      Extremophiles

      Volume: 16 Issue: 2 Pages: 227-236

    • DOI

      10.1007/s00792-011-0422-z

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of cold-indueible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis2012

    • Author(s)
      Park, Jungha, et al
    • Journal Title

      Extremophiles

      Volume: 16 Pages: 227-236

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures2011

    • Author(s)
      Kawamoto, J., Sato, T., Nakasone, K., Kato, C., Mihara, H., Esaki, N., and Kurihara, T
    • Journal Title

      Environmental Microbiology

      Volume: 13 Issue: 8 Pages: 2293-2298

    • DOI

      10.1111/j.1462-2920.2011.02487.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in apiezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures2011

    • Author(s)
      Kawamoto, Jun, et al
    • Journal Title

      Environ.Microbiol.

      Volume: 13 Pages: 2293-2298

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Piezotolerance of the respiratory terminal oxidase activity of the piezophilic Shewanella violacea DSS12 as compared with non-piezophilic Shewanella species2011

    • Author(s)
      Tamegai, Hideyuki, et al
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 75 Pages: 919-924

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Shewanella livingstonensis Ac10の金属呼吸における外膜タンパク質PhoEの機能2014

    • Author(s)
      丸山沙織、川本純、樽井淳、王玉、江崎信芳、栗原達夫
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      川崎市
    • Year and Date
      2014-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 低温菌の環境適応を担う細胞膜脂質2013

    • Author(s)
      栗原達夫、川本純
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2013-11-25
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] 低温菌Shewanella livingstonensis Ac10による金属還元機構の解析2013

    • Author(s)
      丸山沙織、樽井淳、王玉、川本純、栗原達夫
    • Organizer
      極限環境生物学会2013年度(第14回)年会
    • Place of Presentation
      川崎市
    • Related Report
      2013 Final Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 による金属還元機構の解析2013

    • Author(s)
      丸山沙織、樽井淳、王玉、川本純、栗原達夫
    • Organizer
      極限環境生物学会2013年度(第14回)年会
    • Place of Presentation
      川崎市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 地球環境と微生物2012

    • Author(s)
      栗原達夫
    • Organizer
      京都化学者クラブ
    • Place of Presentation
      京都市
    • Year and Date
      2012-10-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Function of phospholipids containing eicosapentaenoic acid in the bacterial cell membrane2012

    • Author(s)
      Tatsuo KURIHARA
    • Organizer
      Wageningen University and Research Centre/Kyoto University Joint Workshop
    • Place of Presentation
      京都市
    • Year and Date
      2012-04-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Function of phospholipids containing eicosapentaenoic acid in the bacterial cell membrane2012

    • Author(s)
      栗原達夫
    • Organizer
      Wageningen University and Research Centre/Kyoto University Joint Workshop
    • Place of Presentation
      京都市・京都大学(招待講演)
    • Year and Date
      2012-04-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 地球環境と微生物2012

    • Author(s)
      栗原達夫
    • Organizer
      京都化学者クラブ
    • Place of Presentation
      京都市 楽友会館
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Shewanella livingstonensis Ac10 の金属呼吸における外膜タンパク質 PhoE の機能

    • Author(s)
      丸山沙織、川本純、樽井淳、王玉、江崎信芳、栗原達夫
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      川崎市
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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