| Project/Area Number |
22405040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Applied veterinary science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
AIDA Yoko 独立行政法人理化学研究所, 分子ウイルス学特別研究ユニット, ユニットリーダー (50182994)
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| Co-Investigator(Renkei-kenkyūsha) |
ONUMA Misao 北海道大学, (連合)獣医学研究科, 名誉教授 (70109510)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | BoLA クラスIIDRB3 / PCR-Sequence based typing(SBT)法 / BLV,BLV nested PCR / フィリピン / ホルスタイン / ブラーマン / Native cattle / BoLAクラスIIDRB3 / PCR-SBT法 / 新規・既知アリル / 東南アジア / 固有品種 / 交雑種 / 多型性 / BoLAクラスII DRB3・DQA1 / BLV nested PCR / 中国山東省 |
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| Research Abstract |
We previously demonstrated that bovine leukocyte antigens (BoLA)-DRB3 alleles associated with resistance or susceptibility to development of bovine leukemia virus (BLV)-induced B lymphoma and analyzed distribution of these alleles in Japan and South-America. However, until now, none of the South-east Asian breed had been characterized for BoLA-DRB3 genes by PCR-sequence based typing (SBT). Hence, we here sequenced exon 2 of the BoLA class II DRB3 gene from 981 cattle including Holstein, Sahiwal, Brahman, Philippine Native and its cross breed, corrected from five islands of Philippine using modified SBT method which is suitable for Philippine cattle. In addition, we detected BLV by a new BLV-CoCoMo-qPCR. We identified the 75 previously reported alleles and 6 novel alleles. Interestingly, the number found in each breed is extent high level, indicating that that Asian breeds reveal extent high level of polymorphism for BoLA-DRB3 distributions. Our result also showed BLV-infected cattle with the susceptibility-associated allele was significantly lower than that in Japan.
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