| Project/Area Number |
22406011
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KANBE Toshio 名古屋大学, 大学院・医学系研究科, 講師 (50093018)
大塚 靖 大分大学, 医学部, 助教 (00244161)
木村 政継 大阪市立大学, 大学院・医学研究科, 准教授 (60195378)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Masatsugu 大阪市立大学, 大学院・医学研究科, 准教授 (60195378)
OTSUKA Yasushi 大分大学, 医学部, 助教 (00244161)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | 国際研究者交流 / インドネシア:ベトナム:カンボジア / 熱帯熱 マラリア原虫 / 東南アジア / 分布領域 / 分子疫学調査 / 遺伝子解析 / 人工 培養 / インドネシア：ベトナム：タイ / 熱帯熱マラリア原虫 / 人工培養 / インドネシア:ミャンマー / インドネシア:パプアニューギニア |
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| Research Abstract |
Our previous findings showed that wild isolates of Plasmodium falciparum collected from Myanmar and Vietnam were easily adapted to in vitro culture, whereas those collected from Indonesian islands failed to be adapted. These results may suggest that Indonesian wild isolates has a tendency not to easily adapt to in vitro culture, differing from those distributing in continental SEA. Therefore, this study was conducted for confirmation of these previous findings and for cytological and molecular epidemiological comparisons between wild isolates collected from continental SEA or from Indonesian islands. All of 20 wild isolates collected from many Indonesian islands could not be adapted to in vitro culture again; all parasites disappeared within 7 days in culture. On the other hand, seven wild isolates collected from Vietnam and Cambodia were adapted to in vitro culture. Particularly, an isolate collected from Vietnam grew well, and it formed many young gametocytes. This isolates could be induced mass-production of gametocytes by decreasing glucose level in the culture medium. By using this isolate and an Indonesian isolate, comparison at molecular level was tried by the Roche Genome Sequencer FLX+ System (the Pyrosequence method), but any difference between them was not found yet.
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