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Molecular epidemiological and cytological studies on Plasmodium falciparum distributing in Southeast Asian continent or island areas

Research Project

Project/Area Number 22406011
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Parasitology (including Sanitary zoology)
Research InstitutionOita University

Principal Investigator

KAWAMOTO Fumihiko  大分大学, 全学研究推進機構, 教授 (40115556)

Co-Investigator(Kenkyū-buntansha) KANBE Toshio  名古屋大学, 大学院・医学系研究科, 講師 (50093018)
大塚 靖  大分大学, 医学部, 助教 (00244161)
木村 政継  大阪市立大学, 大学院・医学研究科, 准教授 (60195378)
Co-Investigator(Renkei-kenkyūsha) KIMURA Masatsugu  大阪市立大学, 大学院・医学研究科, 准教授 (60195378)
OTSUKA Yasushi  大分大学, 医学部, 助教 (00244161)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywords国際研究者交流 / インドネシア:ベトナム:カンボジア / 熱帯熱 マラリア原虫 / 東南アジア / 分布領域 / 分子疫学調査 / 遺伝子解析 / 人工 培養 / インドネシア:ベトナム:タイ / 熱帯熱マラリア原虫 / 人工培養 / インドネシア:ミャンマー / インドネシア:パプアニューギニア
Research Abstract

Our previous findings showed that wild isolates of Plasmodium falciparum collected from Myanmar and Vietnam were easily adapted to in vitro culture, whereas those collected from Indonesian islands failed to be adapted. These results may suggest that Indonesian wild isolates has a tendency not to easily adapt to in vitro culture, differing from those distributing in continental SEA. Therefore, this study was conducted for confirmation of these previous findings and for cytological and molecular epidemiological comparisons between wild isolates collected from continental SEA or from Indonesian islands. All of 20 wild isolates collected from many Indonesian islands could not be adapted to in vitro culture again; all parasites disappeared within 7 days in culture. On the other hand, seven wild isolates collected from Vietnam and Cambodia were adapted to in vitro culture. Particularly, an isolate collected from Vietnam grew well, and it formed many young gametocytes. This isolates could be induced mass-production of gametocytes by decreasing glucose level in the culture medium. By using this isolate and an Indonesian isolate, comparison at molecular level was tried by the Roche Genome Sequencer FLX+ System (the Pyrosequence method), but any difference between them was not found yet.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report

Research Products

(20 results)

All 2012 2011 2010 Other

All Journal Article Presentation Remarks

  • [Journal Article] Preservation of Wild Isolates of Human Malaria Parasites in Wet Ice and Adaptation Efficacy to In Vitro Culture2012

    • Author(s)
      I.S. Tantular, S. Pusarawati, L. Khin, T. Kanbe, M. Kimura, Y. Kido, F. Kawamoto
    • Journal Title

      Trop.Med.Health

      Volume: 40 Issue: 2 Pages: 37-45

    • DOI

      10.2149/tmh.2012-07o

    • NAID

      10031123156

    • ISSN
      1348-8945, 1349-4147
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genotypes of Candida albicans involved in development of candidiasis and their distribution in oral cavity of non-candidiasis individuals2011

    • Author(s)
      Y. Takagi, H. Hattori, H. Adachi, S. Takakura, T. Horii, A. Chindamporn, H. Kitai, R. Tanaka, T. Yaguchi, H. Fukano, F. Kawamoto, K. Shimozato, T. Kanbe
    • Journal Title

      Med. Mycol. J.

      Volume: 52 Pages: 315-324

    • NAID

      10029775696

    • URL

      http://www.jstage.jst.go.jp/brawse/jjmm/-chan/ja/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Micro- satellite-based genotyping of Candida albicans isolated from patients with superficial candidiasis2011

    • Author(s)
      K. Shimizu, H. Hattori, H. Adachi, R. Ohshima, T. Horii, R. Tanaka, T. Yaguchi, M. Akiyama, F. Kawamoto, T. Kanbe
    • Journal Title

      Med Mycol J

      Volume: 52 Pages: 129-138

    • NAID

      10029354557

    • URL

      http://www.jstage.jst.go.jp/brawse/jjmm/-chan/ja/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 溶血発作で発症したグルコース6リン酸脱水素酵素異常症の早産児の 1 例2011

    • Author(s)
      赤澤洋平、馬場 淳、伊藤有香子、斉田 謙、平林香奈枝、三澤 由佳、石田岳史、服部幸夫、藤井寿一、川本文彦、松岡裕之
    • Journal Title

      日本未熟児新生児学会雑誌

      Volume: 23 Pages: 108-112

    • NAID

      10029413972

    • URL

      http://plaza.umin.ac.jp/jspn/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microsatellite-based genotyping of Candida albicans isolated from patien2011

    • Author(s)
      K.Shimizu, et al
    • Journal Title

      Med.Mycology Japan

      Volume: 52 Pages: 129-138

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotypes of Candida albicans involved in development of candidiasis2011

    • Author(s)
      Y.Takagi, et al
    • Journal Title

      Med.Mycology Japan

      Volume: 52 Pages: 315-324

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 溶血発作で発症したグルコース6リン酸脱水素酵素異常症の早産児の1例2011

    • Author(s)
      赤澤陽平, ほか
    • Journal Title

      日本未熟児新生児学会雑誌

      Volume: 23 Pages: 108-112

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single nucleotide poly- morphism, linkage disequilibrium and geographic structure in the malaria parasite Plasmodium vivax2010

    • Author(s)
      P. Orjuela-Sanchez, N.D.Karunaweera, M.Da Silva-Nunes, N.S. da Silva, K.K. Scopel, R.M. Goncalves, C. Amaratunga, J.M. Sa, D. Sochet, R.M. Fairhust, Thava-. kodirasah, G.L. Galapathy, F. Kawamoto, M.U. Ferreira
    • Journal Title

      BMC Genetics

      Volume: 11:65

    • DOI

      10.1186/1471-2156-11-65

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Incidence and mutation analysis of glucose-6-phosphate dehydrogenase deficiencyin eastern Indonesian populations2010

    • Author(s)
      I.S. Tantular, H. Matsuoka, Y. Kasa hara, S Pusarawati, T. Kanbe, J.S. Tuda,Y. Kido, Y.P. Dachlan, F. Kawamoto
    • Journal Title

      Acta Medica Okayama

      Volume: 64 Pages: 367-373

    • URL

      http://escholarship.lib.okayama-u.ac.jp/amo/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single-nucleotide polymorphism, linkage disequilibrium and geographic structure in the malaria parasite Plasmodium vivax.2010

    • Author(s)
      P.Orjuela-Sanchez, et al.
    • Journal Title

      BMC Genetics

      Volume: 2010

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Incidence and mutation analysis of glucose-6-phosphate dehydrogenase deficiency in eastern Indonesian populations2010

    • Author(s)
      I.S.Tantular, et al.
    • Journal Title

      Acta Medica Okayama

      Volume: 64 Pages: 367-373

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 「東南アジアにおけるマラリア の分子疫学的研究」に至った長い道のり2012

    • Author(s)
      川本文彦
    • Organizer
      日本熱帯医学会・学会賞受賞講演
    • Place of Presentation
      仙台
    • Year and Date
      2012-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular epidemiology on malaria and G6PD deficiency in SEA2012

    • Author(s)
      I.S. Tantular & F. Kawamoto
    • Organizer
      ICOPA XII
    • Place of Presentation
      Rio de Janeiro, Brazil
    • Related Report
      2012 Final Research Report
  • [Presentation] A great different characteristics on adaptation to in vitro culture between wild isolates of Plasmodium falciparum collected from continental SEA and from Indonesian islands2012

    • Author(s)
      I.S. Tantular
    • Organizer
      Int. Cong. Parasitol. (ICOPA)
    • Place of Presentation
      Rio de Janeiro
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular epidemiology on G6PD deficiency in southeast Asia2011

    • Author(s)
      F.Kawamoto
    • Organizer
      Int.Symp.Clin.Biomol.Epidemiol.
    • Place of Presentation
      Manado, Indonesia
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular epidemiology of malaria and G6PD deficiency in SEA2011

    • Author(s)
      F. Kawamoto
    • Organizer
      International Symposium on emerging and re-emerging infectious diseases
    • Place of Presentation
      Manado, Indonesia
    • Related Report
      2012 Final Research Report
  • [Presentation] 「東南アジアにおけるマラリアの分子疫学的研究」に至った長い道のり2010

    • Author(s)
      川本文彦
    • Organizer
      日本熱帯医学会
    • Place of Presentation
      仙台市
    • Year and Date
      2010-12-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Population structure of Plasmodium vivax2010

    • Author(s)
      F. Kawamoto
    • Organizer
      The second Int. Meeting on Plasmodium vivax
    • Place of Presentation
      Shanghai, China
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.med.oita-u.ac.jp/rpp/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.med.oita-u.ac.jp/ISR/DSEM/dih/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-22   Modified: 2019-07-29  

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