|Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
We aimed to establish experimental systems to observe BDNF secretion in living animals to elucidate the changes in brain functions associated with BDNF secretion. (1) We tried to express BDNF-pHluorin based on the zebrafish homolog in the visual system of living zebrafish larvae. However, we failed to observe significant fluorescence of BDNF-pHluorin. (2) We successfully expressed BDNF-pHluorin in the mouse hippocampus by lentivirus or electroporation, which enables us to investigate BDNF secretion in relation to memory formation.