Technical maintenance to establish a rabbit sperm bank for human disease models
| Project/Area Number |
22500386
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Saga University |
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Principal Investigator |
KITAJIMA Shuji 佐賀大学, 総合分析実験センター, 准教授 (70284643)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIJIMA Kazutoshi 佐賀大学, 総合分析実験センター, 助教 (70435874)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIJIMA Kazutoshi 佐賀大学, 総合分析実験センター, 助教 (70435874)
FAN Jianglin 山梨大学, 医学工学総合研究部, 教授 (60272192)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 疾患モデル / 遺伝子改変ウサギ / 凍結保存 / バイオリソース / ウサギ / 遺伝子組換え / 遺伝子改変 |
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| Research Abstract |
We developed and improved protocols of rabbit semen cryopreservation and artificial insemination, these protocols are important to establish a rabbit sperm bank of human disease models. We completed cryopreservation of six genetically modified rabbits by frozen semen and made public information of these rabbits on the web site.
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Report
(4 results)
Research Products
(62 results)
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[Journal Article] Delaying embryo development by storing at 4°C for synchronization to recipients in microinjection technique in rabbits.2013
Author(s)
Nishijima, K., Liu, E., Yamaguchi, S., Tanaka, M., Morimoto, M., Watanabe, T., Fan, J. and Kitajima, S.
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Journal Title
Lab. Anim.
Volume: 47
Pages: 145-149
DOI
Related Report
Peer Reviewed
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[Journal Article] Genetic characterization of four wild species of Chinese marmots using microsatellite markers2012
Author(s)
Chen, Y., Wang, Z., Zhang, G., Fan, W., Tao, Y., He, W., Zhao, S., Hung, B., Fan, J., Kitajima, S., Liu, E.
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Journal Title
Biologia
Volume: 67(5)
Pages: 1013-1017
DOI
Related Report
Peer Reviewed
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[Journal Article] Life span of Common Marmoset (Callithrix jacchus) at CLEA Japan breeding colony2012
Author(s)
Nishijima, K., Saitoh, R., Tanaka, S., Ohsato-Suzuki, M., Ohno, T., Kitajima, S.
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Journal Title
Biogerontol.
Volume: 13(4)
Pages: 439-443
DOI
Related Report
Peer Reviewed
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[Journal Article] Effect of the primary cooling rate on the motility and fertility of the frozen-thawed rabbit spermatozoa2012
Author(s)
Maeda, T., Liu, E., Nishijima, K., Tanaka, M., Yamaguchi, S., Morimoto, M., Watanabe, T., Fan, J. and Kitajima, S.
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Journal Title
World Rabbit Sci.
Volume: 20
Pages: 65-70
DOI
Related Report
Peer Reviewed
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[Journal Article] Age is an important factor influence on the number of recovered eggs in superovulated rabbits: Analysis of our past 5 years data of 509 rabbits superovulated by FSH or PMSG. Scand2011
Author(s)
Kitajima, S., Maeda, T., Liu, E., Nishijima, K., Morimoto, M., Watanabe, T. and Fan, J.
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Journal Title
J. Lab. Anim. Sci.
Volume: 38(3)
Pages: 169-174
URL
Related Report
Peer Reviewed
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[Journal Article] Human C-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits2011
Author(s)
Matsuda, S., Yamashita, A., Sato, Y., Kitajima, S., Koike, T., Sugita, C., Moriguchi-Goto, S., Hatakeyama, K., Takahashi, M., Koshimoto, C., Matsuura, Y., Iwakiri, T., Chen, Y.E., Fan, J. and Asada. Y.
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Journal Title
Human C-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits
Volume: 9
Pages: 201-208
Related Report
Peer Reviewed
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[Journal Article] Overexpression of peroxiredoxin 4 protects against high-dose streptozotocin-induced diabetes by suppressing oxidative stress and cytokines in transgenic mice2010
Author(s)
Ding, Y., Yamada, S., Wang, K.Y., Shimajiri, S., Guo, X., Tanimoto, A., Murata, Y., Kitajima, S., Watanabe, T., Izumi, H., Kohno, K., Sasaguri, Y.
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Journal Title
Antioxid. Redox Signal
Volume: 13(10)
Pages: 1477-1490
DOI
Related Report
Peer Reviewed
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[Presentation] Apolipoprotein A-II plays an important role in lipid metabolism2012
Author(s)
Koike, T., Kitajima, S., Nishijima, K., Liu, E., Teruo Watanabe, T., Fan, J.
Organizer
第1回 日中合同ウサギバイオサイエンスフォーラム
Place of Presentation
宮崎
Year and Date
2012-08-04
Related Report
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[Presentation] Assessment of energy expenditure in rabbit with doubly-labeled water method2011
Author(s)
Nishijima, K., Yamada, Y., Tanaka, H., Kitajima, S., Yamaguchi, S., Morimoto, M., Tanaka, K., Nishida, Y.
Organizer
he 4th International Meeting on Rabbit Biotechnology
Place of Presentation
Budapest, Hungary
Related Report
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[Presentation] Apolipoprotein-AII plays an important role in lipid metabolism and atherosclerosis2011
Author(s)
Koike, T., Kitajima, S., Nishijima, K., Liu, E., Watanabe, T., Fan, J.
Organizer
The 4th International Meeting on Rabbit Biotechnology
Place of Presentation
Budapest, Hungary
Related Report
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[Presentation] Human C-reactive Protein Enhances Thrombus Formation In Transgenic Rabbits,(Core 7. Vascular Disease: Biology and Clinical Science, Session Title: Sol Sherry Distinguished Lecture in Thrombosis: New Mechanisms and New Targets in Thrombosis, Abstract 12227)2010
Author(s)
Matsuda, S., Yamashita, A., Kitajima, S., Fan, J., Asada, Y.
Organizer
AHA Scientific Session
Place of Presentation
Chicago, USA
Related Report
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[Presentation] Expression of Human ApoAII in Transgenic Rabbits Leads to Dyslipidemia -A New Model for Combined Hyperlipidemia2010
Author(s)
Koike, T., Kitajima, S., Yu, Y., Nishijima, K., Waqar, AB., Inoue, T., Wang, Y., Zhang, B., Kobayashi, J., Masatoshi, M., Saku, K., Watanabe, T. and Fan, J.
Organizer
The 42nd Annual Scientific Meeting of the Japanese Atherosclerosis Society
Place of Presentation
Gifu
Related Report
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