| Project/Area Number |
22500393
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
|
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| Research Institution | Nihon University |
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Principal Investigator |
都 甲洙 日本大学, 生物資源科学部, 准教授 (40385993)
|
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| Co-Investigator(Kenkyū-buntansha) |
SATO Kahei 日本大学, 生物資源科学部, 教授 (20059778)
|
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 凍結保存 / 生体材料 / 細胞・組織 / 氷結晶 / 耐凍剤 / 近赤外分校イメージング |
|---|
| Research Abstract |
The cryoprotectant is commonly used to protect reproductive tissues during freezing and thawing. As an effort to investigate the osmotic behavior of cryoprotectant, near-infrared (NIR) techniques were applied to monitor the concentration of ethylene glycol (EG) within reproductive tissues. The proposed method provided a novel tool to investigate the optimal freezing method of reproductive tissues on cryoprotectant, ice crystals and cell destruction.
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