Effects of high glucose on physiological function in hypoxic stem cells
| Project/Area Number |
22500687
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | National Defense Medical College |
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Principal Investigator |
ISHIZUKA Toshiaki 防衛医科大学校, 医学教育部医学科専門課程, 准教授 (30399117)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Masanori 防衛医科大学校, 防衛医学研究センター, 准教授 (20525927)
WATANABE Yasuhiro 防衛医科大学校, 医学教育部医学科専門課程, 教授 (90127324)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 高血糖 / 低酸素 / 間葉系幹細胞 / HIF-1α / 酸化ストレス / プロテアゾーム / 幹細胞機能 / 血管新生因子 / スーパーオキサイド / PKC |
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| Research Abstract |
Protein levels of HIF-1αand angiogenic growth factors were attenuated by glucose in hypoxic mouse mesenchymal stem cells in a dose-dependent manner. We also found that stimulation with a high-glucose concentration significantly increased intracellular superoxide levels and proteasome activity in hypoxic stem cells, which may cause HIF-1α degradation. Thus, the modulation of proteasome activity may ameliorate the impaired therapeutic efficacy by autologous bone-marrow-derived cells in diabetic patients.
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Report
(4 results)
Research Products
(60 results)
