| Project/Area Number |
22500731
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Kagawa University |
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Principal Investigator |
KATOH Miyuki 香川大学, 教育学部, 教授 (70112654)
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| Co-Investigator(Kenkyū-buntansha) |
OMORI Masashi 大妻女子大学, 家政学部, 教授 (80074920)
NAGANO Hiroko 岐阜大学, 教育外部, 教授 (40074984)
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| Co-Investigator(Renkei-kenkyūsha) |
KATOH Yoshinobu 北海道立衛生研究所, 研究員 (00414326)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 後発酵茶 / 阿波晩茶 / 微生物 / 乳酸菌 / 抗酸化性 / 乳酸 |
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| Research Abstract |
The separation of microorganisms in a "wooden large bucket" which is an important pointof Awa-bancha was done from the bottom of the bucket in the upper, central and fermentedareas. I was isolated in a selective medium of the various microorganisms isolated. Wereseparated and identified Lactobacillus pentosus, Lactobacillus plantarum, Enterococcus fecium and Enterococcus avium. DNA was extracted from each microorganismisolated using a QIAGEN Dneasy blood & tissue kit, 16S-rRNA analysis was performed using two primers. As a result, I have successfully revealed the nucleotide sequence 1494bp.On the other hand, I have examined the gelatin resolution of microorganisms isolated fromAwa-bancha. Thirty-two microbial species were isolated in the presence of gelatinolytic activity. MIBK layer was investigated by the discoloration of β-carotene coupled with the oxidation of linoleic acid (β-carotene anti-oxidative activity) and the scavenging activity of the DPPH radical.
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