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Exploration of antithrombotic substances and development of new functional foodstuffs considering the circadian rhythms of blood coagulation

Research Project

Project/Area Number 22500774
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionTeikyo University

Principal Investigator

OHKURA Naoki  帝京大学, 薬学部, 准教授 (60349256)

Co-Investigator(Kenkyū-buntansha) OISHI Katsutaka  独立行政法人産業技術総合研究所, 生物機能工学研究部門, 研究グループ長 (50338688)
TANIGUCHI Masahiko  大阪薬科大学, 薬学部, 准教授 (00278590)
ATSUMI Gen-ichi  帝京大学, 薬学部, 教授 (70276608)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords血栓症 / 機能性食品 / 血液凝固 / 日内変動 / 血栓 / 血管内皮細胞 / PAI-1 / HUVEC
Research Abstract

The notion that Angelica keiskei (ashitaba) and propolis have anti-thrombotic properties has not yet been scientifically proven. Here, we show that Ashitaba and propolis have potential as antithrombotic foodstuffs. We also showed that xanthoangerol from ashitaba and chrysin from propolis have antithrombotic properties. However, further investigation is needed to develop new functional foodstuffs involving ashitaba and propolis considering the circadian rhythms of blood coagulation.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (32 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (22 results) Remarks (3 results)

  • [Journal Article] Lipopolysaccharide-induced plasma PAI-1 increase does not correlate with PAI-1 synthesised de novo in the liver2013

    • Author(s)
      Ohkura N, Oishi K, Nakakuki Y, Miura M, Atsumi G
    • Journal Title

      Thrombosis Research

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The molecular clock regulates circadian transcription of tissue factor gene2013

    • Author(s)
      Oishi K, Koyanagi S, Ohkura N
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 431 Issue: 2 Pages: 332

    • DOI

      10.1016/j.bbrc.2012.12.098

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chronic circadian clock disruption induces expression of the cardiovascular risk factor plasminogen activator inhibitor-1 in mice2013

    • Author(s)
      Oishi K, Ohkura N
    • Journal Title

      Blood Coagul Fibrinolysis

      Volume: 24 Issue: 1 Pages: 106

    • DOI

      10.1097/mbc.0b013e32835bfdf3

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Associations between plasma PAI-1 concentrations and its expressions in various organs in obese model mice2012

    • Author(s)
      Ohkura N, Shirakura M, Nakatani E, Oishi K, Atsumi G
    • Journal Title

      Thrombosis Research

      Volume: 130 Issue: 6 Pages: e301-e304

    • DOI

      10.1016/j.thromres.2012.08.297

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Propolis and its constituent chrysin inhibit plasminogen activator inhibitor 1 production induced by tumour necrosis factor-α and lipopolysaccharide2012

    • Author(s)
      Ohkura N, Takata Y, Ando K, Kanai S, Watanabe E, Nohira T, Atsumi G
    • Journal Title

      Journal of Apicultural Research

      Volume: 51 Issue: 2 Pages: 179

    • DOI

      10.3896/ibra.1.51.2.06

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Experimental study on the hemostatc activity of Pollen Typhae: a traditional folk medicine used by external and oral application2011

    • Author(s)
      Ohkura N, Tamura K, Tanaka A, Matsuda J, Atsumi G
    • Journal Title

      Blood Coagul Fibrinolysis

      Volume: 22(8) Issue: 8 Pages: 631-636

    • DOI

      10.1097/mbc.0b013e328349a22c

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xanthoangelols isolated from Angelica keiskei inhibit inflammatory-induced plasminogen activator inhibitor 1 (PAI-1) production2011

    • Author(s)
      Ohkura N, Nakakuki Y, Taniguchi M, Kanai S, Nakayama A, Ohnishi K, Sakata T, Nohira T, Matsuda J, Baba K, Atsumi G
    • Journal Title

      Biofactors

      Volume: 37 Issue: 6 Pages: 455

    • DOI

      10.1002/biof.187

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] プロポリスに含まれるクリシンの抗血栓作用について2012

    • Author(s)
      安藤久美子, 大藏直樹, 高田裕子, 厚味厳一
    • Organizer
      第56回日本薬学会関東支部大会
    • Place of Presentation
      東京 (昭和大学)
    • Year and Date
      2012-10-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 機能性食品による血栓症の予防2012

    • Author(s)
      大藏直樹
    • Organizer
      第一回あしなが予防医学研究会
    • Place of Presentation
      鹿児島 (鹿児島大学)
    • Year and Date
      2012-06-30
    • Related Report
      2012 Final Research Report
  • [Presentation] SennosideA が持つ抗血栓作用の可能性2012

    • Author(s)
      根岸由貴, 大藏直樹, 西井綾香,横内晴菜,三村真里世, 厚味厳一
    • Organizer
      第12回 Pharmaco-Hematology シンポジウム
    • Place of Presentation
      東京 (日本薬学会長井記念館)
    • Year and Date
      2012-06-16
    • Related Report
      2012 Final Research Report
  • [Presentation] あした葉カルコンの血栓症予防効果2012

    • Author(s)
      大藏直樹, 中久木義孝, 松田重三, 厚味厳一
    • Organizer
      第34回日本血栓止血学会
    • Place of Presentation
      東京 (ハイアットリージェンシー東京)
    • Year and Date
      2012-06-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 血栓を作りにくくする天然物成分同定の試み2012

    • Author(s)
      厚味厳一, 大藏直樹
    • Organizer
      日本薬学会第132 年会
    • Place of Presentation
      札幌 (北海道大学)
    • Year and Date
      2012-03-31
    • Related Report
      2012 Final Research Report
  • [Presentation] 血栓を作りにくくする天然物成分同定の試み2012

    • Author(s)
      厚味厳一, 大藏直樹
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌(シンポジウム)(招待講演)
    • Year and Date
      2012-03-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] 生薬からの抗血栓作用を持つ物質の探索2012

    • Author(s)
      根岸由貴, 大藏直樹,西井綾香,横内晴菜,三村真里世,厚味厳一
    • Organizer
      日本薬学会第132 年会
    • Place of Presentation
      札幌 (北海道大学)
    • Year and Date
      2012-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 生薬からの抗血栓作用を持つ物質の探索2012

    • Author(s)
      根岸由貴, 大藏直樹, 西井綾香, 横内晴菜, 三村真里世, 厚味厳一
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] あした葉カルコンの血栓症予防効果2012

    • Author(s)
      大藏直樹
    • Organizer
      第34回 日本血栓止血学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] SennosideAが持つ抗血栓作用の可能性2012

    • Author(s)
      根岸由貴
    • Organizer
      第12回Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] プロポリスに含まれるクリシンの抗血栓作用について2012

    • Author(s)
      安藤久美子
    • Organizer
      第56回日本薬学会関東支部大会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Xanthoangelols isolated from Angelica keiskei inhibit inflammatory-induced plasminogen activator inhibitor 1 (PAI-1) production ISTH20112011

    • Author(s)
      大藏直樹, 中久木義孝, 谷口 雅彦,金井志帆, 大西克典, 阪田敏幸, 野平知良, 松田重三 , 馬場きみ江 , 厚味厳一
    • Organizer
      XXIII Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      京都 (京都国際会館)
    • Year and Date
      2011-07-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Xanthoangelols isolated from Angelica keiskei inhibit inflammatory-induced plasminogen activator inhibitor 1 (PAI-1) production2011

    • Author(s)
      大藏直樹, 中久木義孝, 谷口雅彦, 金井志帆, 大西克典, 阪田敏幸, 野平知良, 松田重三, 馬場きみ江, 厚味厳一
    • Organizer
      ISTH2011 (XXIII Congress of the International Society on Thrombosis and Haemostasis)
    • Place of Presentation
      Kyoto
    • Year and Date
      2011-07-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] ブラジル産プロポリス中のフラボノイドが持つ抗血栓作用2011

    • Author(s)
      安藤久美子
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2010 Annual Research Report
  • [Presentation] 天然物成分による血液凝固系の調節2011

    • Author(s)
      大藏直樹
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2010 Annual Research Report
  • [Presentation] TSOD マウスを用いた肥満による血中 PAI-1 上昇と脂肪組織 PAI-1 の関連解明2010

    • Author(s)
      大藏直樹
    • Organizer
      第6回 TSOD (肥満・糖尿病)マウス研究会情報交換会
    • Place of Presentation
      東京 (総評会館)
    • Year and Date
      2010-11-05
    • Related Report
      2012 Final Research Report
  • [Presentation] プロポリスによるプラスミノーゲンアクチベーターインヒビター1(PAI-1)産生の抑制2010

    • Author(s)
      大藏直樹, 安藤久美子, 高田裕子, 西井綾香, 横内晴菜, 福迫莉衣, 渡辺恵美子, 金井志帆, 野平知良, 松田重三, 厚味厳一
    • Organizer
      第15回日本フードファクター学会
    • Place of Presentation
      仙台 (仙台市民会館)
    • Year and Date
      2010-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] プロポリスのプラスミノーゲンアクチベーターインヒビター1(PAI-1)産生抑制作用2010

    • Author(s)
      大藏直樹, 安藤久美子, 高田裕子, 西井綾香, 横内晴菜, 福迫莉衣, 渡辺恵美子,金井志帆, 野平知良, 松田重三, 厚味厳一
    • Organizer
      第54回日本薬学会関東支部大会
    • Place of Presentation
      東京 (東京薬科大学)
    • Year and Date
      2010-10-02
    • Related Report
      2012 Final Research Report
  • [Presentation] プロポリスのプラスミノーゲンアクチベーターインヒビター1(PAI-1)産生抑制作用2010

    • Author(s)
      大藏直樹
    • Organizer
      第54回日本薬学会関東支部大会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] プロポリスによるプラスミノーゲンアクチベーターインヒビター1(PAI-1)産生の抑制2010

    • Author(s)
      大藏直樹
    • Organizer
      第15回日本フードファクター学会
    • Place of Presentation
      仙台
    • Related Report
      2010 Annual Research Report
  • [Presentation] (震災のため誌上開催)天然物成分による血液凝固系の調節静岡

    • Author(s)
      大藏直樹, 厚味厳一
    • Organizer
      日本薬学会第131 年会
    • Related Report
      2012 Final Research Report
  • [Presentation] (震災のため誌上開催)ブラジル産プロポリス中のフラボノイドが持つ抗血栓作用

    • Author(s)
      安藤久美子,大藏直樹,高田裕子,西井綾香,横内晴菜,福迫莉衣,渡辺恵美子,金井志帆,野平知良,松田重三, 厚味厳一
    • Organizer
      日本薬学会第131 年会
    • Related Report
      2012 Final Research Report
  • [Remarks] 平成 22年度 富山大学 和漢医薬学総合研究所 共同利用・共同研究拠点 共同研究

    • URL

      http://wakandb.u-toyama.ac.jp/wiki/Report:Report2010

    • Related Report
      2012 Final Research Report
  • [Remarks] 大藏直樹, 大石勝隆 (2012) 血栓症と体内時計 THE CHEMICAL TIMES 2012 No.3 225巻 平成 24年7月発行

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://wakandb.u-toyama.ac.jp/wiki/Report:Report2010

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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