Identification of microRNA and non-coding RNA regulating the oncogenesis and the progression of colorectal cancers
| Project/Area Number |
22501015
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIBATA Kohei 九州大学, 大学病院, 准教授 (50363560)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Toyoki 九州大学, 大学病院, 准教授 (30264112)
三森 功士 九州大学, 生体防御医学研究所, 准教授 (50322748)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | がん遺伝子 / ncRNA / 8q24 / PICT1 / 19q13 / mTOR / XIST / 染色体再構成 / lincRNA / 大腸癌 / 癌幹細胞 / 癌関連遺伝子多型 / マイクロアレイ / laser microdissection / aCGH / HOTAIR |
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| Research Abstract |
We have focused on novel non-coding RNAs being associated with the oncogenesis and progression of CRC. We applied our established database (aCGH, Gene Expression array, SNP array) to identify the significant molecule by using of the in silico analysis. As a result, we have eventually disclosed three findings;
1) Down-regulation of miR-144 leads to poor prognosis of CRC patients via activation of the mTOR signaling pathway.
2) Expressions of several miRs reflects genomic alterations in colorectal cancer cells. Simultaneous changes of cluster miRs originated form genomic alteration can lead to crucial difference of protein regulation mechanisms continuously, and to influence cancer progression.
3) We demonstrated that G/T rs6983267 allelic variants differently affect lincRNA expression in response to Wnt signaling, and identified MYC as a lincRNA target. Our work suggests a new mechanism for the different CRC risk in subjects carrying the rs6983267 variations.
By grace of the grant, we identified important ncRNA as a candidate clinical marker, and disclosed a new basic oncogenic mechanism in CRC.
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Report
(4 results)
Research Products
(87 results)
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[Journal Article] Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma2013
Author(s)
Ishibashi M, Kogo R, Shibata K,Sawada G, Takahashi Y, Kurashige J, Akiyoshi S, Sasaki S, Iwaya T, Sudo T, Sugimachi K, Mimori K, Wakabayashi G, Mori M
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Journal Title
Oncol Rep
Volume: 29(3)
Pages: 946-50
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[Journal Article] Plastin3 is a novel marker for circulating tumor cells undergoing the epithelial-mesenchymal transition and is associated with colorectal cancer prognosis2013
Author(s)
Yokobori T, Iinuma H, Shimamura T, Imoto S, Sugimachi K, Ishii H, Iwatsuki M, Ota D, Ohkuma M, Iwaya T, Nishida N, Kogo R, Sudo T, Tanaka F, Shibata K, Toh H, Sato T, Barnard GF, Fukagawa T, Yamamoto S, Nakanishi H, Sasaki S, Miyano S, Watanabe T, Kuwano
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Journal Title
Cancer Res
Volume: 73(7)
Pages: 2059-69
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[Journal Article] Clinical Significance of PICT1 in Patients of Hepatocellular Carcinoma with Wild-Type TP532013
Author(s)
Ishibashi M, Kogo R, Shibata K, Ueo H, Uchi R, Matsumura T, Takano Y, Sawada G, Takahashi Y, Mima K, Kurashige J, Akiyoshi S, Iwaya T, Eguchi H, Sudo T, Sugimachi K, Suzuki A, Wakabayashi G, Mori M, Mimori K
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Journal Title
Related Report
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[Journal Article] Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance2012
Author(s)
Hashiguchi Y, Nishida N, Mimori K, Sudo T, Tanaka F, Shibata K, Ishii H, Mochizuki H, Hase K, Doki Y, Mori M
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Journal Title
Int J Oncol
Volume: 40(5)
Pages: 1477-82
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[Journal Article] Microarray analysis of colorectal cancer stromal tissue reveals upregulation of two oncogenic miRNA clusters2012
Author(s)
Nishida N, Nagahara M, Sato T, Mimori K, Sudo T, Tanaka F, Shibata K, Ishii H, Sugihara K, Doki Y, Mori M
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Journal Title
Clin Cancer Res
Volume: 18(11)
Pages: 3054-70
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[Journal Article] MicroRNA-372 is associated with poor prognosis in colorectal cancer2012
Author(s)
Yamashita S, Yamamoto H, Mimori K, Nishida N, Takahashi H, Haraguchi N, Tanaka F, Shibata K, Sekimoto M, Ishii H, Doki Y, Mori M
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Journal Title
Oncology
Volume: 82(4)
Pages: 205-12
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[Journal Article] Expression of Mesenchymal Markers Vimentin and Fibronectin: The Clinical Significance in Esophageal Squamous Cell Carcinoma2012
Author(s)
Sudo T, Iwaya T, Nishida N, Sawada G, Takahashi Y, Ishibashi M, Shibata K,Fujita H, Shirouzu K, Mori M, Mimori K
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Journal Title
Related Report
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[Journal Article] Downregulation of miR-144 is associated with colorectal cancer progression via activation of mTOR signaling pathway2012
Author(s)
Iwaya T, Yokobori T, Nishida N, Kogo R, Sudo T, Tanaka F, Shibata K, Sawada G, Takahashi Y, Ishibashi M, Wakabayashi G, Mori M, Mimori K
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Journal Title
Carcinogenesis
Volume: 33(12)
Pages: 2391-7
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[Journal Article] Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance.2012
Author(s)
Yojiro Hashiguchi, Naohiro Nishida, Koshi Mimori, Tomoya Sudo, Fumiaki Tanaka, Kohei Shibata, Hideshi Ishii, Hidetaka Mochizuki, Kazuo Hase, Yuichiro Doki, Masaki Mori
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Journal Title
Int. J. Oncol
Volume: 40(5)
Pages: 1477-1482
DOI
Related Report
Peer Reviewed
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[Journal Article] Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers2011
Author(s)
Kogo R, Shimamura T, Mimori K, Kawahara K, Imoto S, Sudo T, Tanaka F, Shibata K, Suzuki A, Komune S, Miyano S, Mori M
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Journal Title
Cancer Res
Volume: 71(20)
Pages: 6320-6
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[Journal Article] Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers2011
Author(s)
Kogo R, Shimamura T, Mimori K, Kawahara K, Imoto S, Sudo T, Tanaka F, Shibata K, Suzuki A, Komune S, Miyano S, Mori M
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Journal Title
Cancer Research
Volume: 71
Pages: 6320-6
DOI
Related Report
Peer Reviewed
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[Journal Article] MicroRNA-125a-5p is an independent prognostic factor in gastric cancer,and inhibits the proliferation of human gastric cancer cells in combination with trastuzumab.2011
Author(s)
Nishida N, Mimori K, Fabbri M, Yokobori T, Sudo T, Tanaka F, Shibata K, Ishii H, Doki Y, Mori M.]
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Journal Title
Clin Cancer Res
Volume: 17(9)
Pages: 2725-2733
DOI
Related Report
Peer Reviewed
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[Journal Article] The physical ability of Japanese female elderly with cerebrovascular disease correlates with the telomere length and subtelomeric methylation status in their peripheral blood leukocytes2011
Author(s)
Maeda, T.Oyama, J.Higuchi, Y.Nishiyama, Y.Kudo, Y.Yamori, T.Nakazono, T.Arima, T.Mimori, K., et al.
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Journal Title
Gerontology
Volume: 57
Pages: 137-43
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Peer Reviewed
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[Journal Article] Strong interaction between the effects of alcohol consumption and smoking on oesophageal squamous cell carcinoma among individuals with ADH1B and/or ALDH2 risk alleles2010
Author(s)
Tanaka, F.Yamamoto, K.Suzuki, S.Inoue, H.Tsurumaru, M.Kajiyama, Y.Kato, H.Igaki, H.Furuta, K.Fujita, H.Tanaka, T.Tanaka, Y.Kawashima, Y.Natsugoe, S.Setoyama, T.Tokudome, S.Mimori, K., et al.
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Peer Reviewed
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