Analysis of regulatory mechanisms of DNA repair by ubiquitination

• Project/Area Number: 22510059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: MOTEGI Akira 京都大学, 大学院・医学研究科, 助教 (80452332)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: DNA修復 / 複製後修復 / 相同組換え / ユビキチン / Rad5

Research Abstract

I have studied functions of the ubiquitin ligases SHPRH and RNF20 by genetically manipulatingthose genes in the chicken DT40 cell line. SHPRH/RAD18 double knockout cells showed enhancedsensitivity towards the DNA-crosslinking agent cisplatin, suggesting a cooperative role of SHPRH andRAD18 in repairing DNA crosslinks. Repression of the RNF20 transgene in RNF20-disrupted cellsresulted in reduced foci formation of the recombination factor RAD51 after ionizing radiation. Thus,RNF20 may facilitate repair of DNA double strand breaks by homologous recombination.

Research Products

• [Journal Article] Multiple genetic manipulations of DT40 cell line (2013)
  • Author(s): Akira Motegi
  • Journal Title: Methods in Molecular Biology Volume: 未定
• [Journal Article] The SUMO protease SENP1is required for cohesion maintenanceand mitotic arrest following spindlepoison treatment (2012)
  • Author(s): Era S, Abe T, Arakawa H, Kobayashi S,Szakal B, Yoshikawa Y, Motegi A, TakedaS, Branzei D
  • Journal Title: Biochem Biophys ResCommun Volume: 426 Pages: 310-6
  • Peer Reviewed
• [Journal Article] The SUMO protease SENP1 is required for cohesion maintenance and mitotic arrest following spendle poison treatment (2012)
  • Author(s): Saho Era
  • Journal Title: Biochem. Biophys. Res. Comm. Volume: 426 Issue: 3 Pages: 310-316
  • DOI: 10.1016/j.bbrc.2012.08.066
  • Peer Reviewed
• [Journal Article] Dpb11/TopBP1 plays distinct roles inDNA replication (2011)
  • Author(s): Germann SM, Oestergaard VH, Haas C,Salis P, Motegi A, Lisby M.
  • Journal Title: checkpoint responseand homologous recombination Volume: 10 Pages: 210-224
  • Peer Reviewed
• [Journal Article] Dpb11/TopBP1 plays distinct roles in DNA replication, checkpoint response and homologous recombination (2011)
  • Author(s): Germann SM, Oestergaard VH, Haas C, Salis P, Motegi A, Lisby M
  • Journal Title: DNA Repair Volume: 10 Pages: 201-224
  • Peer Reviewed
• [Journal Article] Simultaneous disruption of two DNApolymerases, Polη and Polζ, in avianDT40 cells unmasks the role of Polη incellular response to various DNAlesions (2010)
  • Author(s): Hirota K, Sonoda E, Kawamoto T, MotegiA, Masutani C, Hanaoka F, Szuts D, IwaiS, Sale JE, Lehmann A, Takeda S
  • Journal Title: PLoS Genet Volume: 6
  • Peer Reviewed
• [Journal Article] Simultaneous Disruption of Two DNA Polymerases, Polη and Polζ, in Avian DT40 Cells Unmasks the Role of Polη in Cellular Response to Various DNA Lesions (2010)
  • Author(s): Hirota K, Sonoda E, Kawamoto T, Motegi A, Masutani C, Hanaoka F, Szuts D, Iwai S, Sale JE, Lehmann A, Takeda S
  • Journal Title: PLoS Genetics Volume: 6(10)
  • NAID: 120002661519
  • Peer Reviewed
• [Journal Article] Multiple geneticmanipulations of DT40 cell line
  • Author(s): Motegi A, Takata M
  • Journal Title: Methods in Molecular Biology Volume: (in press)
• [Presentation] DNA損傷応答ネットワークにおけるリン酸化・ユビキチン化修飾ダイナミクスのプロテオーム解析 (2010)
  • Author(s): 足立淳、鳴海良平、佐野聖三、久家貴寿、白水崇、松本雅記、中山敬一、茂木章、井倉毅、高田穣、朝長毅
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-10