Exploration of chemical probes for investigation of oxidative metabolism of auxins
Project/Area Number |
22510228
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Living organism molecular science
|
Research Institution | Kyoto University |
Principal Investigator |
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | オーキシン / 異化代謝 / 質量分析 / スクリーニング / 酸化酵素 / indole-3-acetic acid / oxidation / inhibitor / screening / 植物ホルモン |
Research Abstract |
Indole-3-acetic acid is a class of plant hormones, which is responsible for plant growth regulation. The concentration of IAA is strictly controlled by metabolism and transport. In IAA metabolism, oxidation plays an important role, but its mechanisms are not fully understood. In this study, we developed a screening system for inhibitors of IAA oxidation. As a result, we could establish a high-throughput screening system using LC/MS/MS to detect oxidative metabolites and evaluated the action of the known inhibitor of IAA oxidation in rice.
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Report
(4 results)
Research Products
(11 results)