Neutron structure analysis of human PGD synthase for elucidation of the reaction mechanism activated by metal ions
| Project/Area Number |
22550152
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
INOUE Tsuyoshi 大阪大学, 大学院・工学研究科, 教授 (20263204)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Hiroyoshi 大阪大学, 大学院・工学研究科, 准教授 (30324809)
MIZOHATA Eiichi 大阪大学, 大学院・工学研究科, 助教 (90571183)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 中性子線解析 / 金属イオン / 酵素活性化機構 / 中性子線構造解析 / プロスタグランジンＤ合成酵素 / 反応メカニズムの解明 / 結晶の大型育成 / プロスタグランジンD合成酵素 / 反応メカニズムの明 |
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| Research Abstract |
Prostaglandin D_2(PGD_2) is an allergic and inflammatory mediator produced by hematopoietic PGDS (H-PGDS) in the presence of glutathione (GSH). Previously, we found that Ca^2+and Mg^2+activated the enzyme activity and uncovered the activation mechanism by the X-ray crystal structures of the Ca^2+- and Mg^2+-bound forms of human H-PGDS at high resolution. In this study, we performed the crystallization of H-PGDS to reveal the protonation state by neutron diffraction and obtained the crystals with maximum size of 0.5 mm^3with high quality.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Effects of a forced solution flow on the step advancement on {110} faces of tetragonal lysozyme crystals: direct visualization of individual steps under a forced solution flow2012
Author(s)
M. Maruyama, K. Hisato, G. Sazaki, S. Maki, Y. Takahashi, H. Yoshikawa, S. Sugiyama, H. Adachi, K. Takano, H. Matsumura, T. Inoue, S. Murakami & Y. Mori
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Journal Title
Cryst. Growth Des.
Volume: 12
Pages: 2856-2863
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[Journal Article] Spatially precise, soft microseeding of single protein crystals by femtosecond laser ablation2012
Author(s)
H. Yoshikawa, Y. Hosokawa, R. Murai, G. Sazaki, T. Kitatani, H. Adachi, T. Inoue, H. Matsumura, K. Takano, S. Murakami, S. Nakabayashi, Y. Mori & H. Matsumura
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Journal Title
Cryst. Growth Des.
Volume: 12
Pages: 4334-4339
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[Presentation] Novel Approach for Growth of High-Quality and Large Protein Crystals2010
Author(s)
H. Matsumura, K. Kakinouchi, N. Shimizu, S. Sugiyama, Y. Takahashi, M. Maruyama, R. Murai, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori
Organizer
3th International Conference on the Crystallization of Biological Macromolecules
Place of Presentation
Dublin(Ireland)
Related Report
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[Presentation] Protein Crystallization in Agarose Gel with High Strength2010
Author(s)
S. Sugiyama, M. Hirose, R. Murai, Y. Takahashi, M. Maruyama, G. Sazaki, H. Adachi, K. Takano, S. Murakami, T. Inoue, H. Matsumura
Organizer
3th International Conference on the Crystallization of Biological Macromolecules
Place of Presentation
Dublin(Ireland)
Related Report
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