Modification of chloroplast proteins with reactive aldehydes: its implication in the plant response to oxidative stress

• Project/Area Number: 22570044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Yamaguchi University
• Principal Investigator: MANO Junichi 山口大学, 大学研究推進機構, 教授 (50243100)
• Co-Investigator(Kenkyū-buntansha): MATSUI Kenjhi 山口大学, 大学院・医学系研究科, 教授 (90199729) ; TANAKA Kiyoshi 鳥取大学, 農学部, 教授 (50124350)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 光合成 / 酸化ストレス / 環境応答 / アクロレイン / タンパク質修飾 / 酸化シグナル / 過酸化脂質 / 活性カルボニル種 / オキシリピン / ヒドロキシノネナール / 酸化タンパク質 / 塩ストレス / タンパク質カルボニル化 / 葉緑体 / 4-オキソ-2-ヘキセナール / 環境ストレス / クロトンアルデヒド

Research Abstract

We obtained following results about the action of lipid peroxide-derived reactive aldehydes (RAL) in plant responses to environmental stress:
(1) The RAL species responsible for the aluminum-induced oxidative injury in roots and those for the strong-light-induced photooxidative injury in leaves identified.
(2) Light-dependent formation of organic peroxides on the donor side of photosystem II that lacked the water-oxidizing complex was proven.
(3) In NaCl-stressed plants, modification of proteins with RAL were increased. The modified targets were identified.
(4) The target amino acid(s) in phosphoribulokinase that have primary importance in the sensitivity to acrolein was not the redox-regulated Cys.

Research Products

• [Journal Article] Modulation of activity and inhibitor sensitivity of rabbit aldose reductase-like protein (AKR1B19) by oxidized glutathione and SH-reagents (2013)
  • Author(s): Endo S., Fujimoto, A., Kumada, S., Matsunaga, T., Ohno S., Mano, J., Tajima, K., El-Kabbani, O., Hara, A
  • Journal Title: Chem. Biol. Interact Volume: 202 Pages: 146-152
  • Peer Reviewed
• [Journal Article] Modulation of activity and inhibitor sensitivity of rabbit aldose reductase-like protein (AKR1B19) by oxidized glutathione and SH-reagents (2013)
  • Author(s): Satoshi Endo
  • Journal Title: Chemico-Biological Interactions Volume: 202 Issue: 1-3 Pages: 146-152
  • DOI: 10.1016/j.cbi.2012.11.026
  • Peer Reviewed
• [Journal Article] Characterization of rabbit aldose reductase-like protein with 3 -hydroxysteroid dehydrogenase activit (2012)
  • Author(s): Endo, S., Matsunaga, T., Kumada, S., Fujimoto, A., Ohno, S., El-Kabbani, O., Hu, D., Toyooka, N., Mano, J., Tajima, K. and Hara, A
  • Journal Title: Arch. Biochem. Biophys Volume: 527 Pages: 23-30
  • Peer Reviewed
• [Journal Article] Differential metabolism of green leaf volatiles in injured and intact parts of a wounded leaf meet distinct ecophysiological requirements (2012)
  • Author(s): Matsui, K., Sugimoto, K., Mano, J., Ozawa, R. and Takabayashi, J
  • Journal Title: PLoS One Volume: 7
  • NAID: 120004288160
  • Peer Reviewed
• [Journal Article] Reactive carbonyl species: their production from lipid peroxides, action in environmental stress, and the detoxification mechanism (2012)
  • Author(s): Mano, J
  • Journal Title: Plant Physiol. Biochem Volume: 59 Pages: 90-97
  • Peer Reviewed
• [Journal Article] Reactive carbonyl species : their production from lipid peroxides, action in environmental stress, and the detoxification mechanism (2012)
  • Author(s): Mano, J.
  • Journal Title: Plant Physiol.Biochem. Volume: 59 Pages: 90-97
  • DOI: 10.1016/j.plaphy.2012.03.010
  • Peer Reviewed
• [Journal Article] Differential Metabolisms of Green Leaf Volatiles in Injured and Intact Parts of a Wounded Leaf Meet Distinct Ecophysiological Requirements (2012)
  • Author(s): Matsui K, Sugimoto K, Mano J, Ozawa R, Takabayashi J
  • Journal Title: PLoS ONE Volume: 7 Issue: 4 Pages: 36433-36433
  • DOI: 10.1371/journal.pone.0036433
  • Peer Reviewed
• [Journal Article] Characterization of rabbit aldose reductase-like protein with 3b-hydroxysteroid dehydrogenase activity (2012)
  • Author(s): Satoshi Endo
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 527 Issue: 1 Pages: 23-30
  • DOI: 10.1016/j.abb.2012.07.012
  • Peer Reviewed
• [Journal Article] Differential metabolism of green leaf volatiles in injured and intact parts of a wounded leaf meet distinct ecophysiological requirements (2012)
  • Author(s): Matsui, K., Sugimoto, K., Mano, J., Ozawa, R., Takabayashi, J.
  • Journal Title: PLoS One
  • NAID: 120004288160
  • Peer Reviewed
• [Journal Article] Photoproduction of catalase-insensitive peroxides on the donor side of manganese-depleted photosystem II: evidence with a specific fluorescent probe (2011)
  • Author(s): Yanykin, D. V., Ivanov, B. N., Klimov, V. V. and Mano, J
  • Journal Title: Biochemistry Volume: 50 Pages: 10658-10665
  • Peer Reviewed
• [Journal Article] Characterization of raspberry ketone/zingerone synthase, catalyzing the alpha,beta-hydrogenation of phenylbutanones in raspberry fruits (2011)
  • Author(s): Koeduka, T., Watanabe, B., Suzuki, S., Hiratake, J., Mano, J. and Yazaki, K
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 412 Pages: 104-108
  • Peer Reviewed
• [Journal Article] Photoproduction of catalase-insensitive peroxides on the donor side of manganese-depleted photosystem II : evidence with a specific fluorescent probe (2011)
  • Author(s): Khoeobrykh, S., Khorobrykh, A., Yankykin, D., Ivanov, B, Klimov, V, Mano, J.
  • Journal Title: Biochemistry Volume: 50 Pages: 10658-10665
  • Peer Reviewed
• [Journal Article] Characterization of raspberry ketone/zingerone synthase, catalyzing the alpha, beta-hydrogenation of phenylbutanones in raspberry fruits (2011)
  • Author(s): Koeduka, T., Watanabe, B., Suzuki, S., Hiratake, J., Mano, J., Yazaki, K.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 412 Pages: 104-108
  • Peer Reviewed
• [Journal Article] Accumulation of lipid peroxide-derived, toxic , -unsaturated aldehydes (E)-2-pentenal, acrolein and (E)-2-hexenal in leaves under photoinhibitory illumination (2010)
  • Author(s): Mano, J., Tokushige, K., Mizoguchi, H., Fujii, H. and Khorobrykh, S
  • Journal Title: Plant Biotechnol Volume: 27 Pages: 193-197
  • Peer Reviewed
• [Journal Article] The involvement of lipid peroxide-derived aldehydes in aluminum toxicity of tobacco roots (2010)
  • Author(s): Yin, L., Mano, J., Wang, S., Tsuji, W. and Tanaka, K
  • Journal Title: Plant Physiol Volume: 152 Pages: 1406-1417
  • Peer Reviewed
• [Presentation] 塩ストレスにより活性カルボニル修飾を受けるタンパク質の同定 (2013)
  • Author(s): 真野純一,永田光曜,岡村星多郎,白矢武士,三ツ井敏明
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2013-03-23
• [Presentation] Protein modification with reactive carbonyl species: its implication in the plant responses to environmental stress (2013)
  • Author(s): Mano, J
  • Organizer: 日本植物生理学会シンポジウム「Photo-oxidative stress on the chloroplasts and molecular dynamics of thylakoids
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2013-03-22
• [Presentation] Protein modification with reactive carbonyl species: its implication in the plant responses to environmental stress (2013)
  • Author(s): Jun'ichi Mano
  • Organizer: 日本植物生理学会シンポジウム「Photo-oxidative stress on the chloroplasts and molecular dynamics of thylakoids」
  • Place of Presentation: 岡山大学（岡山市）
  • Invited
• [Presentation] 塩ストレスにより活性カルボニル修飾を受けるタンパク質の同定 (2013)
  • Author(s): 真野純一
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山市）
• [Presentation] Lipid peroxide-derived carbonyls mediate oxidative injury of plant cells in environmental stress (2012)
  • Author(s): Mano, J
  • Organizer: On The 4th Asian Symposium on Plant Lipid
  • Place of Presentation: 香港(中国)
  • Year and Date: 2012-12-04
• [Presentation] Reactive carbonyl species induce programmed cell death in tobacco cells (2012)
  • Author(s): Biswas, Md. Sanaullah, Mano, J
  • Organizer: Reactive carbonyl species induce programmed cell death in tobacco cells
  • Place of Presentation: 山口大学工学部(宇部市)
  • Year and Date: 2012-09-22
• [Presentation] ターゲットメタボロミクスとターゲットプロテオミクスによる植物ストレス傷害過程の解析 (2012)
  • Author(s): 真野純一
  • Organizer: 山口大学大学研究推進機構第1回研究発表会
  • Place of Presentation: 山口大学(山口市)
  • Year and Date: 2012-09-21
• [Presentation] 過酸化脂質由来活性カルボニル種のグルタチオンによる解毒 (2012)
  • Author(s): 石橋亜沙美,永田光曜,真野純一
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学(姫路市)
  • Year and Date: 2012-09-17
• [Presentation] 葉緑体ストロマ酵素の脂質由来カルボニル種に対する感受性の網羅的解析 (2012)
  • Author(s): 伊藤達也,弘田智,真野純一
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学(姫路市)
  • Year and Date: 2012-09-17
• [Presentation] 塩ストレス下の葉で活性カルボニル種により修飾を受けるタンパク質の同定 (2012)
  • Author(s): 岡村星多郎,永田光曜,白矢武士,三ツ井敏明,真野純一
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学(姫路市)
  • Year and Date: 2012-09-17
• [Presentation] ウサギのアルドース還元酵素類似タンパク質は 3-ヒドロキシステロイド脱水粗酵素活性を示す. (2012)
  • Author(s): 熊田翔,藤本愛理,遠藤智史,松永俊之,原明,田島和男,真野純一
  • Organizer: 第76回生化学会中部支部例会
  • Place of Presentation: 岡崎コンファレンスセンター(岡崎市)
  • Year and Date: 2012-05-26
• [Presentation] Sergey A. Khorobrykh, Andrei A. Khorobrykh:光化学系 II 酸化側での有機ペルオキシド生成 (2012)
  • Author(s): 真野純一
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2012-03-18
• [Presentation] 光化学系II酸化側での有機ペルオキシド生成 (2012)
  • Author(s): 真野純一, Sergey A.Khorobrykh, Andrei A.Khorobrykh
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学,京都市
  • Year and Date: 2012-03-17
• [Presentation] 葉緑体ストロマ酵素の脂質由来カルボニル種に対する感受性の網羅的解析 (2012)
  • Author(s): 伊藤達也
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学工学部（姫路市）
• [Presentation] 過酸化脂質由来活性カルボニル種のグルタチオンによる解毒 (2012)
  • Author(s): 石橋亜沙美
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学工学部（姫路市）
• [Presentation] Reactive carbonyl species induce programmed cell death in tobacco cells (2012)
  • Author(s): Biswas Md. Sanaullah
  • Organizer: 日本農芸化学会中四国支部第34回講演会
  • Place of Presentation: 山口大学工学部（宇部市）
• [Presentation] Lipid peroxide-derived carbonyls mediate oxidative injury of plant cells in environmental stress (2011)
  • Author(s): Mano, J.
  • Organizer: The 4th Asian Symposium on Plant Lipids
  • Place of Presentation: 香港,・中国
  • Year and Date: 2011-12-03
• [Presentation] 脂質由来強毒性ケトアルデヒド 4-oxo-2-hexenal の葉緑体での生成と光合成への影響 (2011)
  • Author(s): 真野純一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館,京都
  • Year and Date: 2011-09-24
• [Presentation] 脂質由来強毒性ケトアルデヒド4-oxo-2-hexenalの葉緑体での生成と光合成への影響 (2011)
  • Author(s): 真野純一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場,京都市
  • Year and Date: 2011-09-24
• [Presentation] Reactive carbonyl species as mediators of oxidative stress (2011)
  • Author(s): Mano, J
  • Organizer: On 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants
  • Place of Presentation: ブダペスト(ハンガリー)
  • Year and Date: 2011-07-08
• [Presentation] Reactive carbonyl species as mediators of oxidative stress (2011)
  • Author(s): Mano, J.
  • Organizer: 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants
  • Place of Presentation: ブダペスト,ハンガリー
  • Year and Date: 2011-07-05
• [Presentation] リノレン酸過酸化物由来ケトアルデヒド4-oxo-4-hexenal による潜在的な光合成阻害とその消去 (2011)
  • Author(s): 真野純一,弘田智,永田光曜,松井健二
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] リノレン酸過酸化物由来ケトアルデヒド4-oxo-4-hexenalによる潜在的な光合成阻害とその消去 (2011)
  • Author(s): 真野純一
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-21
• [Presentation] 活性カルボニル種:もうひとつの酸化ストレスメディエーター (2011)
  • Author(s): 真野純一
  • Organizer: 大阪大学蛋白質研究所セミナー「分子科学と生理学が解き明かす植物の光エネルギー変換の新展開」
  • Place of Presentation: 大阪大学蛋白質研究所
  • Year and Date: 2011-03-10
• [Presentation] Oxylipin aldehydes as cell-injuring agents in environmental stress. (2010)
  • Author(s): 真野純一
  • Organizer: BMB2010
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-08
• [Presentation] 葉緑体で生成するオキシリピンアルデヒドの同定 (2010)
  • Author(s): 真野純一
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口大学
  • Year and Date: 2010-05-15
• [Presentation] 環境ストレス下で生じるオキシリピンアルデヒドによる葉緑体酵素の失活 (2010)
  • Author(s): 弘田智
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口大学
  • Year and Date: 2010-05-15
• [Presentation] 植物はヘキセナールを葉面から吸収し,直ちに還元する (2010)
  • Author(s): 松井健二
  • Organizer: 第54回香料・テルペンおよび精油化学に関する討論会
  • Place of Presentation: 山梨大学
• [Presentation] Formation of highly reactive aldehydes from polyunsaturated fatty acids in chloroplasts. (2010)
  • Author(s): Mano, J.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜
• [Presentation] Inactivation of stroma enzymes by oxylipin aldehydes generated under environmental stress. (2010)
  • Author(s): Hirota, T.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜