Elucidation of the molecular mechanism of the sleep regulation by fatty acid binding protein FABP7

• Project/Area Number: 22570081
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Osaka Bioscience Institute
• Principal Investigator: NAGATA Nanae 公益財団法人大阪バイオサイエンス研究所, 分子行動生物学部門, 研究員 (80390805)
• Co-Investigator(Renkei-kenkyūsha): FUJIMORI Ko 大阪薬科大学, 薬学部, 講師 (70425453) ; KASHIWAGI Kaori 公益財団法人大阪バイオサイエンス研究所, 分子行動生物学部門, 研究員 (10372666)
• Research Collaborator: MIYAMOTO Etsuko 東京大学医科学研究所東京大学医科学研究所, インタラクトーム医科学社会連携研究部門, 部門長 特任准教授 (70327708)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: FABP7 / 脂肪酸 / 睡眠

Research Abstract

プロスタグランジンなどの脂質（脂肪酸）は睡眠調節に関与することが知られている。一方で、睡眠は疲労回復や記憶の定着などに重要とされるが、これら睡眠の生理的意義の分子機構は解明されていない。本研究では、マウス大脳皮質でのmRNA発現レベルが明期と暗期で変動する脂肪酸輸送タンパク質brain fatty acid binding protein (FABP7の相互作用タンパク質としてGlycoprotein M6a (GPM6a)を同定した。更に、両タンパク質がマウス脳でも相互作用していることを確認し、FABP7とGPM6aがマウス脳で相互作用し機能している可能性を示した。

Research Products

• [Journal Article] Endogenous sleep-promoting substance (2012)
  • Author(s): Nagata N, Urade Y
  • Journal Title: Nihon Rinsho Volume: 70 Pages: 1227-1232
  • NAID: 40019360568
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22844810
• [Journal Article] Orally administered rubiscolin-6, a δ opioid peptide derived from Rubisco, stimulates food intake vialeptomeningeal lipocallin-type prostaglandin D synthase in mice. (2012)
  • Author(s): Kaneko K, Lazarus M, Miyamoto C, Dishi Y, Nagata N, Yang S. Yoshikawa M. Aritake K, Furuyashiki T, Narumiya S, Urade Y, Chinata K.
  • Journal Title: Mol Nutr Food Res. Volume: 56(8) Issue: 8 Pages: 1315-1323
  • DOI: 10.1002/mnfr.201200155
  • Peer Reviewed
• [Journal Article] Increased expression of lipocalin-type-prostaglandin D synthase in ulcerative colitis and exacerbating role in murine colitis. (2011)
  • Author(s): Hokari R, Kurihara C, Nagata N, et al.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol. Volume: 300
  • Peer Reviewed
• [Journal Article] Catalytic mechanism of the primary human prostaglandin F(2a)synthase, aldo-keto reductase 1B1-prostaglandin D(2)synthase activity in the absence of NADP(H). (2011)
  • Author(s): Nagata N, Kusakari Y, Fukunishi Y, Inoue T, Urade Y.
  • Journal Title: FEBS J. Volume: 278 Pages: 1288-1298
  • Peer Reviewed
• [Presentation] Identification of new interacting partners of FABP7 in the mouse brain (2012)
  • Author(s): Nanae Nagata
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2012-12-15
• [Presentation] Generation and Characterization of a monoclonal antibody against mouse DP1 (2011)
  • Author(s): Nanae Nagata, Toshiyoshi Yamamoto, Kaori Kashiwagi, Michael Lazarus, Yoshihiro Urade
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] A novel SOX5 splicing isoform expressed in mouse brain during sleep (2011)
  • Author(s): Nanae Nagata
  • Organizer: World Sleep 2011
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-04
• [Presentation] Prostaglandin D2 Synthase Activity Catalyzed by Human Aldo-Keto Reductase 1B1 in the Absence of NADPH (2010)
  • Author(s): Nagata N, Kusakari Y, Fukunishi Y, Inoue T, Urade Y.
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Function of a novel SOX5 splicing isoform expressed in mouse brain during sleep (2010)
  • Author(s): Nanae Nagata
  • Organizer: Neuro2010
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-09-02