| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Using tandem affinity purification and mass spectrometry, a lot of host factor candidates that interact with the movement protein (MP) of Red clover necrotic mosaic virus (RCNMV) were identified from Nicotiana benthamianaplants. Among these, full-length cDNA of Glyceraldehyde 3-phosphate dehydrogenase（GAPDH-A）was cloned. GAPDH-Awas shown to be the host factor that is involved in the cell-to-cell movement of RCNMV. From the detailed study of GAPDH-A intracellular localization, I have revealed that GAPDH-Apositively regulates the colocalization of the MP and the replication complex to support efficientcell-to-cell movement of the virus.
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