Functional analysis of Xanthomonas oryzae pv. Oryzae type lll secretion Protein LrpX, which functions both in the plant and the bacterium

• Project/Area Number: 22580053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kyoto Prefectural University
• Principal Investigator: TSUGE Seiji 京都府立大学, 大学院・生命環境科学研究科, 准教授 (10254319)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: イネ白葉枯病菌 / hrp / エフェクター / 植物-細菌相互作用 / 植物－細菌相互作用 / 植物防御応答

Research Abstract

A Xanthomonas oryzae pv. oryzae protein LrpX was found to function as an effector secreted through a type III secretion system into rice cells along with other effectors XopR, XopY and XopAA. Protein LrpX is likely to be associated with lipoxygenase and to represses the defense system via the jasmonate signaling cascade. Also, I identified two novel hrp regulators, XrvB and Lon protease, and found that LrpX also functions as a hrp negative regulator downstream of the proteins in the bacterial cell.

Research Products

• [Journal Article] A Receptor-like Cytoplasmic Kinase Targeted by a Plant Pathogen Effector Is Directly Phosphorylated by the Chitin Receptor and Mediates Rice Immunity. (2013)
  • Author(s): Yamaguchi, K, Yamada, K, Ishikawa, K, et al.
  • Journal Title: Cell Host Microbe Volume: 13(3) Issue: 3 Pages: 347-57
  • DOI: 10.1016/j.chom.2013.02.007
  • Peer Reviewed
• [Journal Article] Suppression of Rice Immunity by <i>Xanthomonas oryzae</i> Type III Effector Xoo2875 (2013)
  • Author(s): Koji Yamaguchi, Yusuke Nakamura, Kazuya Ishikawa, Yuya Yoshimura, Seiji Tsuge and Tsutomu Kawasaki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 4 Pages: 796-801
  • DOI: 10.1271/bbb.120929
  • NAID: 10031170721
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] a type III effector secreted by Xanthomonas oryzae pv. Oryzae (2012)
  • Author(s): Chiharu Akimoto-Tomiyama, Ayako Furutani, Seiji Tsuge, Erika J. Washington, Yoko Nishizawa, Eiichi Minami and Hirokazu Ochiai XopR
  • Journal Title: suppresses microbe-associated molecular pattern-triggered immunity inArabidopsis thaliana. Molecular Plant-Microbe Interactions Volume: 25 Issue: 4 Pages: 505-514
  • DOI: 10.1094/mpmi-06-11-0167
  • Peer Reviewed
• [Journal Article] An H-NS-like protein involved in negative regulation of hrp genes in Xanthomonas oryzae pv. Oryzae (2011)
  • Author(s): Yumi Kametani-Ikawa, Seiji Tsuge,Ayako Furutani and Hirokazu Ochiai
  • Journal Title: FEMS MicrobiologyLetters Volume: 319 Issue: 1 Pages: 58-64
  • DOI: 10.1111/j.1574-6968.2011.02266.x
  • Peer Reviewed
• [Presentation] ユビキチンリガーゼであるOsPUB44 の植物免疫における機能解析 (2013)
  • Author(s): 石川和也
  • Organizer: 平成25年度 日本植物病理学会大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2013-03-28
• [Presentation] ユビキチンリガーゼであるOsPUB44の植物免疫における機能解析 (2013)
  • Author(s): 石川和也
  • Organizer: 平成25年度 日本植物病理学会大会
  • Place of Presentation: 岐阜県岐阜市
• [Presentation] イネ白葉枯病菌の Lonプロテアーゼは hrp 遺伝子発現の負の制御に関与する (2012)
  • Author(s): 亀谷(伊川)有美
  • Organizer: 平成24年度 日本植物病理学会関西部会
  • Place of Presentation: 鳥取市
  • Year and Date: 2012-09-27
• [Presentation] イネ白葉枯病菌の環境応答と病原性に関わる遺伝子発現機構 (2012)
  • Author(s): 津下誠治
  • Organizer: 平成24年度植物感染生理談話会
  • Place of Presentation: 近江八幡市
  • Year and Date: 2012-08-30
• [Presentation] イネ白葉枯病菌の環境応答と病原性に関わる遺伝子発現機構 (2012)
  • Author(s): 津下誠治
  • Organizer: 平成24年度 植物感染生理談話会
  • Place of Presentation: 滋賀県近江八幡市
  • Invited
• [Presentation] イネ白葉枯病菌のLonプロテアーゼはhrp遺伝子発現の負の制御に関与する (2012)
  • Author(s): 亀谷（伊川）有美
  • Organizer: 平成24年度 日本植物病理学会関西部会
  • Place of Presentation: 鳥取県鳥取市
• [Presentation] Involvement of H-NS-like protein XrvB in the negative regulation of hrp genes in Xanthomonas oryzae pv.oryzae (2011)
  • Author(s): Tsuge, S., 他
  • Organizer: 第13回国際細菌・応用微生物学会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-07
• [Presentation] nvolvement of H-NS-like protein XrvB in the negative regulation of hrp genes in Xanthomonas oryzae pv. Oryzae (2011)
  • Author(s): Yumi Kametani-Ikawa
  • Organizer: 第13回国際細菌・応用微生物学会
  • Place of Presentation: 札幌市
• [Remarks]
  • URL: http://www2.kpu.ac.jp/life_environ/plant_path/
• [Remarks] 京都府立大学植物病理学研究室
  • URL: http://www2.kpu.ac.jp/life_environ/plant_path/
• [Remarks]
  • URL: http://www2.kpu.ac.jp/life_environ/plant_path/
• [Remarks]
  • URL: http://www2.kpu.ac.jp/life_environ/plant_path/