| Project/Area Number |
22580066
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
TOYOTA Koki 東京農工大学, 大学院・農学研究院, 教授 (30262893)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 有機物管理 / 生物防除 / リアルタイムPCR / Heterodera glycines / 孵化 / 食品廃棄物 / シスト / 定量PCR / 有機性廃棄物 / eDNA |
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| Research Abstract |
Effects of the application of bean sprout residue to soil on the soybean cyst nematode (SCN), Heterodera glycines, were evaluated in pot experiments. When bean sprout residue was applied into a soil infested with SCN at a rate of 1%, the number of SCN juveniles (J2), measured with the Baermann method, markedly increased after 14 days of application and then markedly decreased after 35 days, suggesting that some eggs in cysts were hatched by a stimulant in the bean sprout and then starved to death in the absence of host. Next, bean sprout residue was applied four times and the number of SCN after 7 weeks was estimated with the real-time PCR method which enables to quantify all the forms of SCN (egg, J2, cyst). Results showed that the SCN density was not changed in control, while decreased by more than 70% in the residue treatment. Such a hatching-stimulatory and subsequent eradication effect was observed in the water extract of bean sprout residue. Among different parts of bean sprout, the root parts had a higher stimulatory effect for hatching. These results revealed that bean sprout residue might function as an environmental-friendly control measure for SCN. A field experiment confirmed that damage caused by SCN was significantly suppressed by the addition of bean sprout residue 3 weeks before transplanting. A problem is that hatching stimulation occurred only at soil temperature around 25C and therefore timing for the application of bean sprout residue is limited.
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