| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
We carried out comprehensive analysis of the biosynthetic machinery and their regulatory system for lankacidin and lankamycin production in Streptomyces rochei 7434AN4. The disruptant of lkmE encoding type-II thioesterase accumulated 15-nor-LM derivatives, suggesting LkmE is responsible for hydrolysis of aberrant starter units for LM biosynthesis. We elucidated the structure of the signaling molecules SRBs that induce LC and LM production. Furthermore, the extensive analysis of regulatory genes revealed the positive regulation of LM biosynthesis by two SARP genes.
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