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Searching of environmental stress markers of kelp by protein profiling

Research Project

Project/Area Number 22580192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

YOTSUKURA Norishige  北海道大学, 北方生物圏フィールド科学セ ンター, 准教授 (60312344)

Co-Investigator(Kenkyū-buntansha) MORIMOTO Kouichi  近畿大学, 生物理工学部, 教授 (10319741)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsバイオマーカー / プロテオーム / Saccharina japonica / 環境ストレス / 藻場保全 / マコンブ
Research Abstract

The number and the amount of protein that changes expression level by temperature and nutrient stresses were investigated on Saccharina japonica. As the result, 47 spots that increase the expression level under high water temperature and 7 spots that decrease under the condition were detected. And, a few spots that increase under oligotrophic conditions were found. When the electrophoretic profiles were compared, it was thought that some of them were corresponding to already-known proteins that increased under high water temperature. Hence, those use as environmental stress marker is expected.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (49 results)

All 2013 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 14 results) Presentation (28 results) (of which Invited: 1 results) Book (4 results)

  • [Journal Article] Investigation of proteomic profiles of lamina of Ecklonia kurome (Laminariales): homology-based cross-species protein identification and analysis of the post-translational processing of vanadium-dependent bromoperoxidases using MALDI-TOF/TOF2013

    • Author(s)
      Kouhei Nagai, Koichi Morimoto, Haruka Ikegami, Hajime Kimura, Norishige Yotsukura
    • Journal Title

      Marine Biotechnology

      Volume: online first Issue: 4 Pages: 487-498

    • DOI

      10.1007/s10126-013-9498-z

    • NAID

      120005460240

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effective DNA extraction method for fragment analysis using capillary sequencer of the kelp, Saccharina.2013

    • Author(s)
      Takashi Maeda, Tadashi Kawai, Masahiro Nakaoka and Norishige Yotsukura.
    • Journal Title

      Journal of Applied Phycology

      Volume: 25 Issue: 1 Pages: 337-347

    • DOI

      10.1007/s10811-012-9868-3

    • NAID

      120005228165

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] アイヌによるコンブ採り及び利用に関する情報2012

    • Author(s)
      川井唯史・ Louis D. Druehl・四ツ倉典滋.
    • Journal Title

      Algal Resources

      Volume: 5 Pages: 77-81

    • NAID

      130008048020

    • URL

      http://jsap.web.fc2.com/pub.html

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ホソメコンブの鮮度保持技術の開発2012

    • Author(s)
      木下康宣・野上智代・赤石恵・大坪雅史・鳥海滋・吉野博之・大野一・川下浩一・秋野秀樹・四ツ倉典滋.
    • Journal Title

      北海道立工業技術センター研究報告

      Volume: 12 Pages: 6-11

    • NAID

      220000145043

    • URL

      http://www.techakodate.or.jp/center/information/report/h22.html

    • Related Report
      2012 Final Research Report
  • [Journal Article] Temperature stress-induced changes in the proteomic profiles of Ecklonia cava (Laminariales, Phaeophyceae)2012

    • Author(s)
      Norishige Yotsukura, Kouhei Nagai, Toshimitsu Tanaka, Hajime Kimura and Kouichi Morimoto
    • Journal Title

      Journal of Applied Phycology

      Volume: 24 Issue: 2 Pages: 163-171

    • DOI

      10.1007/s10811-011-9664-5

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ホソメコンブの鮮度保持技術の開発2012

    • Author(s)
      木下康宣
    • Journal Title

      北海道立工業技術センター研究報告

      Volume: 印刷中

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Taxonomy and biogeography of Agarum and Thalassiophyllum (Laminariales, Phaeophyceae) based on nuclear, mitochondrial, and plastid gene sequences.2011

    • Author(s)
      Ga Hun Boo, Sandra C. Lindstrom, Nina G. Klochkova, Norishige Yotsukura, Eun Chan Yang, Hyung Geun Kim, J. Robert Waaland, Ga Youn Cho, Kathy Ann Miller, and Sung Min Boo.
    • Journal Title

      Taxon

      Volume: 60 Issue: 3 Pages: 831-840

    • DOI

      10.1007/s10811-009-9452-7

    • URL

      http://www.ingentaconnect.com/content/iapt/tax/2011/00000060/00000003;jsessionid=1gxhvriuufxhh.alexandra

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Taxonomy and biogeography of Agarum and Thalassiophyllum2011

    • Author(s)
      Ga Hun Boo, Sandra C.Lindstrom, Nina G.Klochkova, Norishige Yotsukura, Eun Chan Yang
    • Journal Title

      TAXON

      Volume: 60 Pages: 831-840

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ロシア、サハリン州と日本におけるガゴメコンブの分布に関する情報2011

    • Author(s)
      Galanin Dmitry、四ツ倉典滋、川井唯史
    • Journal Title

      Algal Resources

      Volume: 4 Pages: 1-8

    • NAID

      130008048005

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature stress-induced changes in the proteomic profiles of Ecklonia cava (Laminariales, Phaeophyceae)2011

    • Author(s)
      Norishige Yotsukura
    • Journal Title

      Journal of Applied Phycology

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 日本産コンブ目植物の分類体系2011

    • Author(s)
      四ツ倉典滋
    • Journal Title

      Algal Resources

      Volume: (印刷中)

    • NAID

      130008077507

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Taxonomy and biogeography of Agarum and Thalassiophyllum (Laminariales, Phaeophyceae) based on nuclear, mitochondrial, and plastid gene sequences2011

    • Author(s)
      Ga Hun Boo
    • Journal Title

      Taxon

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal changes in proteomic profiles of Japanese kelp: Saccharina japonica (Laminariales, phaeophyceae)2010

    • Author(s)
      Norishige Yotsukura, Kouhei Nagai, Hajime Kimura and Kouichi Morimoto.
    • Journal Title

      Journal of Applied Phycology

      Volume: 22 Issue: 4 Pages: 443-451

    • DOI

      10.1007/s10811-009-9477-y

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本産コンブ目植物の 分類体系.2010

    • Author(s)
      四ツ倉典滋.
    • Journal Title

      Algal Resources

      Volume: 3 Pages: 193-198

    • NAID

      130008077507

    • URL

      http://jsap.web.fc2.com/pub.html

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 生鮮ホソメコンブの鮮度評価方法に関する研究2010

    • Author(s)
      木下康宣・野上智代・赤石恵・大坪雅史・鳥海滋・吉野博之・大野一・川下浩一・秋野秀樹・舟橋正浩・四ツ倉典滋.
    • Journal Title

      北海道立工業技術センター研究報告

      Volume: 11 Pages: 12-16

    • URL

      http://www.techakodate.or.jp/center/information/report/h22.html

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Journal Article] Mitochondrial DNA Sequence variation of four Saccharina species (Laminariales, Phaeophyceae) growing in Japan2010

    • Author(s)
      Norishige Yotsukura
    • Journal Title

      Journal of Applied Phycology

      Volume: 22 Pages: 243-251

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal changes in proteomic profiles of Japanese kelp : Saccharina japonica (Laminariales, phaeophyceae)2010

    • Author(s)
      Norishige Yotsukura
    • Journal Title

      Journal of Applied Phycology

      Volume: 22 Pages: 443-451

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] コンブ多型解析に有効なマイクロサテライトマーカーの探索2013

    • Author(s)
      前田高志.
    • Organizer
      日本応用藻類学会第11回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2013-03-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 北海道沿岸におけるコンブ藻場の長期変動2013

    • Author(s)
      渡辺健太郎.
    • Organizer
      日本生態学会第6回大会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡市)
    • Year and Date
      2013-03-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 型計量魚探の開発(4)コンブ林の磯焼けの動態把握に向けて2012

    • Author(s)
      邵花梅
    • Organizer
      平成24年度日本水産学会秋季大会
    • Place of Presentation
      水産大学校(下関市)ー研究報告
    • Year and Date
      2012-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 北海道におけるコンブ類研究2012

    • Author(s)
      四ツ倉典滋.
    • Organizer
      日本藻類学会第35回大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-07-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 青森県日本海沿岸に生育するホンダワラ属5種の季節的消長の比較2012

    • Author(s)
      藤川義一
    • Organizer
      日本応用藻類学会第11回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2012-03-24
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 冷凍保存中におけるコンブの加熱緑変機能の変化2012

    • Author(s)
      木下康宣
    • Organizer
      日本応用藻類学会第11回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2012-03-24
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] コンブ多型解析に有効なマイクロサテライトマーカーの探索2012

    • Author(s)
      前田高志、川井唯史、仲岡雅裕、四ツ倉典滋
    • Organizer
      日本応用藻類学会第11回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Elucidation of genetic diversity of Saccharina japonica in northern part of Japan based on DNA fragment analyses.2011

    • Author(s)
      Takashi Maeda.
    • Organizer
      VIth Asian P. acific Phycological Forum
    • Place of Presentation
      The Ocean Resort, Yeosu (Korea)
    • Year and Date
      2011-10-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Elucidation of genetic diversity of Saccharina japonica in northern part of Japan based on DNA fragment analyses2011

    • Author(s)
      Takashi Maeda, Tadashi Kawai, Yoshikazu Fujikawa, Shinji Kirihara, Masahiro Nakaoka, Norishige Yotsukura
    • Organizer
      VIth Asian Pacific Phycological Forum
    • Place of Presentation
      The Ocean Resort, Yeosu (Korea)
    • Year and Date
      2011-10-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] "こんぶ"を知る、"こんぶ"について考える~伝統産業の発展と地域の活性化を目指して~2011

    • Author(s)
      四ツ倉典滋
    • Organizer
      平成23年度北海道信用金庫理事長会議
    • Place of Presentation
      渡島信用金庫(北海道森町)(招待講演)
    • Year and Date
      2011-09-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] AFLP分析によるマコンブ産地間の遺伝的多様性の解明と変種識別2011

    • Author(s)
      前田高志.
    • Organizer
      日本応用藻類学会第10回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-07-09
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 養殖アカモクに認められた2年目への再生2011

    • Author(s)
      藤川義一
    • Organizer
      日本応用藻類学会第10回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-07-09
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 生鮮コンブの鮮度評価方法に関する研究2011

    • Author(s)
      木下康宣
    • Organizer
      日本応用藻類学会第10回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-07-09
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 生鮮コンブの鮮度保持技術に関する研究2011

    • Author(s)
      木下康宣
    • Organizer
      日本応用藻類学会第10回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-07-09
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] A trial on molecular marker detection for variety discrimination in Saccharina japonica (Phaeophyceae, Laminariales) by AFLP-based analysis.2011

    • Author(s)
      Takashi Maeda
    • Organizer
      4th Congress of the International Society for Applied Phycology
    • Place of Presentation
      Halifax Marriott Harbourfront (Canada)
    • Year and Date
      2011-06-21
    • Related Report
      2012 Final Research Report
  • [Presentation] A trial on molecular marker detection for variety discrimination in Saccharina japonica (Phaeophyceae, Laminariales) by AFLP-based analysis2011

    • Author(s)
      Maeda I.,Pang S.J., Yotsukura N
    • Organizer
      4th Congress of the International Society for Applied Phycology
    • Place of Presentation
      The Halifax Marriott Harbourfront Hotel, Halifax(Canada)
    • Year and Date
      2011-06-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] コンブ分類の現状と課題-変わりゆく分類体系をまえにして-2011

    • Author(s)
      四ツ倉典滋.
    • Organizer
      日本藻類学会第35回大会公開講座「富山県民のための昆布学」.
    • Place of Presentation
      富山大学(富山市)
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] コンブ分類の現状と課題-変わりゆく分類体系をまえにして-2011

    • Author(s)
      四ツ倉典滋
    • Organizer
      日本藻類学会第35回大会公開講座「富山県民のための昆布学」
    • Place of Presentation
      富山大学(富山県) 招待講演
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 北太平洋西部におけるコンブ類の多様性2010

    • Author(s)
      四ツ倉典滋.
    • Organizer
      北海道大学大型融合プロジェクト研究支援ワークショップ「環北太平洋沿岸生物相研究の新展開-歴史検証と未来予測-
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2010-11-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 北太平洋西部におけるコンブ類の多様性2010

    • Author(s)
      四ツ倉典滋
    • Organizer
      北海道大学大型融合プロジェクト研究支援ワークショップ「環北太平洋沿岸生物相研究の新展開-歴史検証と未来予測-」
    • Place of Presentation
      北海道大学(北海道函館市) 招待講演
    • Year and Date
      2010-11-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Production of kelp in Japan: various natural resources and established aquaculture technique.2010

    • Author(s)
      Norishige Yotsukura.
    • Organizer
      An international interdisciplinary Symposium
    • Place of Presentation
      Carlsberg Academy (Denmark)
    • Year and Date
      2010-08-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Production of kelp in Japan : various natural resources and established aquaculture technique2010

    • Author(s)
      Norishige Yotsukura
    • Organizer
      An international interdisciplinary Symposium
    • Place of Presentation
      Copenhagen (Denmark) 招待講演
    • Year and Date
      2010-08-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] コンブ類の多様性とその保全2010

    • Author(s)
      四ツ倉典滋
    • Organizer
      第22回千葉県立中央博物館自然史シンポジウム「宮部金吾生誕 150 周年記念日本の藻類学は今!」
    • Place of Presentation
      千葉県立中央博物館(千葉市)
    • Year and Date
      2010-07-17
    • Related Report
      2012 Final Research Report
  • [Presentation] コンブ類の多様性とその保全2010

    • Author(s)
      四ツ倉典滋
    • Organizer
      第22回千葉県立中央博物館自然史シンポジウム「宮部金吾生誕150周年記念日本の藻類学は今!」
    • Place of Presentation
      千葉県立中央博物館(千葉県) 招待講演
    • Year and Date
      2010-07-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] マルバアマノリの分類形質2010

    • Author(s)
      前田高志
    • Organizer
      第9回日本応用藻類学会春季シンポジウム
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2010-07-03
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] 北海道におけるコンブ類研究

    • Author(s)
      四ツ倉典滋
    • Organizer
      日本藻類学会第35回大会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 型計量魚探の開発④ コンブ林の磯焼けの動態把握に向けて

    • Author(s)
      邵花梅
    • Organizer
      平成24年度日本水産学会秋季大会
    • Place of Presentation
      水産大学校(下関市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 北海道沿岸におけるコンブ藻場の長期変動

    • Author(s)
      渡辺健太郎
    • Organizer
      日本生態学会第60回大会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡市)
    • Related Report
      2012 Annual Research Report
  • [Book] えりも町ふるさと再発見シリーズ4えりも町と日高管内の「海の生き物たち」(えりも町郷土資料館編)2011

    • Author(s)
      四ツ倉典滋
    • Publisher
      えりも町教育委員会
    • Related Report
      2012 Final Research Report
  • [Book] えりも町ふるさと再発見シリーズ4 えりも町と日高管内の「海の生き物たち」(えりも町郷土資料館編)2011

    • Author(s)
      四ツ倉典滋, ほか
    • Publisher
      えりも町教育委員会
    • Related Report
      2011 Annual Research Report
  • [Book] 水産海洋ハンドブック2010

    • Author(s)
      四ツ倉典滋
    • Publisher
      生物研究社
    • Related Report
      2012 Final Research Report
  • [Book] 水産海洋ハンドブック2010

    • Author(s)
      四ツ倉典滋(共著)
    • Publisher
      生物研究社
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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