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Development of a conventional diagnostic method for Eurytrema and conducting an epidemiological survey on grazing cattle in South Kyushu

Research Project

Project/Area Number 22580351
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionUniversity of Miyazaki

Principal Investigator

NONAKA Nariaki  宮崎大学, 農学部, 准教授 (50281853)

Co-Investigator(Kenkyū-buntansha) HORII Yoichiro  宮崎大学, 農学部, 教授 (80173623)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords疫学・膵蛭 / 分子生物学 / 膵蛭 / 小型膵蛭 / 形態 / DNA / 分布 / 診断 / 疫学 / PCR / 小形膵蛭 / 虫卵検査 / Eurytrema / 系統樹解析 / 18S rRNA
Research Abstract

Two methods for diagnosis of Eurytrema pancreaticum and E. coelomaticum, called nylon-mesh filtration egg detection method and coproantigen detection method, were developed. DNA analysis on the trematodes collected in Japan, Brazil and Vietnam revealed that the two species of Eurytrema are the independent species, however, they could not be distinguished each other completely by their morphology. For treatment of the infection, it was found that one oral administration with 25 mg/kg of praziquantel was effective.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (14 results)

All 2013 2012 2011

All Journal Article (2 results) Presentation (10 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Application of nylon mesh in fecal examination for Eurytrema eggs.2012

    • Author(s)
      K. Sakaguchi, T. Shinohara, H. Sakai, N. Shoji, Y. Horii, N. Nonaka
    • Journal Title

      Japanese Journal of Veterinary Parasitology

      Volume: 10 Pages: 21-21

    • Related Report
      2012 Final Research Report
  • [Journal Article] Application of nylon mesh in fecal examination for Eurytrema eggs2012

    • Author(s)
      Sakaguchi, K.
    • Journal Title

      Japanese Journal of Veterinary Parasitology

      Volume: 10 Pages: 21-21

    • Related Report
      2012 Annual Research Report
  • [Presentation] 食肉由来寄生虫症2013

    • Author(s)
      野中成晃
    • Organizer
      平成24年度宮崎県食肉衛生検査所協議会研修会
    • Place of Presentation
      宮崎県総合保健センター(宮崎県)
    • Year and Date
      2013-02-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 食肉由来寄生虫症2013

    • Author(s)
      野中成晃
    • Organizer
      平成24年度宮崎県食肉衛生検査所協議会研修会
    • Place of Presentation
      宮崎県総合保健センター(宮崎県)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 膵蛭卵検査におけるナイロンメッシュの活用2012

    • Author(s)
      酒井博史 下田崇 酒井由紀夫 野中成晃
    • Organizer
      平成24年度家畜診療等技術九州地区発表会
    • Place of Presentation
      福岡県中小企業振興センター(福岡県)
    • Related Report
      2012 Final Research Report
  • [Presentation] 膵蛭Eurytrema pancreaticum と小形膵蛭E. coelomaticumの遺伝学的および形態学的鑑別の検討2012

    • Author(s)
      坂口浩平、Pham Ngoc Doanh、郭志宏、甘甜甜、昌子暢賢、山本裕子、新田芳樹、岡島淳悟、Itabajara da Silva Vaz Junior、今内覚、堀井洋一郎、野中成晃
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学(岩手県)
    • Related Report
      2012 Final Research Report
  • [Presentation] 膵蛭Eurytrema pancreaticum と小形膵蛭E. coelomaticumの遺伝学的検討および形態学的鑑別について2012

    • Author(s)
      坂口浩平、Pham Ngoc Doanh、郭志宏、甘甜甜、昌子暢賢、山本裕子、新田芳樹、岡島淳悟、Itabajara da Silva Vaz Junior、今内覚、堀井洋一郎、野中成晃
    • Organizer
      第6回蠕虫研究会
    • Place of Presentation
      青島サンクマール(宮崎県)
    • Related Report
      2012 Final Research Report
  • [Presentation] 膵蛭Eurytrema pancreaticum と小形膵蛭E. coelomaticumの遺伝学的検討および形態学的鑑別について2012

    • Author(s)
      坂口浩平
    • Organizer
      第6回蠕虫研究会
    • Place of Presentation
      青島サンクマール(宮崎県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 膵蛭Eurytrema pancreaticum と小形膵蛭E. coelomaticumの遺伝学的および形態学的鑑別の検討2012

    • Author(s)
      坂口浩平
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学(岩手県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 膵蛭卵検査におけるナイロンメッシュの活用2012

    • Author(s)
      酒井博史
    • Organizer
      平成24年度家畜診療等技術九州地区発表会
    • Place of Presentation
      福岡県中小企業振興センター(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ナイロンメッシュを用いた膵蛭類の虫卵検査法の効率化2011

    • Author(s)
      坂口浩平、篠原呂之、酒井博史、昌子暢賢、堀井洋一郎、野中成晃
    • Organizer
      第64回日本寄生虫学会南日本支部大会・第61回日本衛生動物学会南日本支部大会合同大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県)
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 膵蛭類の虫卵検査におけるナイロンメッシュの活用2011

    • Author(s)
      坂口浩平、篠原呂之、酒井博史、昌子暢賢、堀井洋一郎、野中成晃
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      大阪府立大学(大坂府)
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Book] 獣医寄生虫病学2013

    • Author(s)
      野中成晃.吸虫総論
    • Total Pages
      160
    • Publisher
      緑書房
    • Related Report
      2012 Final Research Report
  • [Book] 獣医寄生虫病学2013

    • Author(s)
      野中成晃
    • Total Pages
      160
    • Publisher
      緑書房
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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