| Project/Area Number |
22590152
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Kinjo Gakuin University |
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Principal Investigator |
KURATA Yoko 金城学院大学, 薬学部, 助教 (80513928)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Takaaki 名古屋大学, 医学(系)研究科(研究院), 教授 (70314010)
AMIOKA Katsuo 金城学院大学, 薬学部, 教授 (50387594)
KUZUYA Takafumi 名古屋大学, 医学部附属病院, 副部長 (00444406)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | B 細胞 / 薬力学解析 / 免疫抑制薬 / 感受性試験 / 免疫機能モニタリング / B細胞 / 免疫抑制学 |
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| Research Abstract |
Recently, it has been revealed that the B cells related toanti-donor antibody production have an important role in chronic rejection after organtransplantation. Our attention has been directed towards detailed evaluation of variousimmunosuppressive drugs using pharmacodynamic (PD) analysis of B cell as well as T cell. We attempted to explore the useful combination of PD analysis of B cell andpreviously-established CFSE flow cytometry-based T cell proliferation assay. Obtained results were as follows, (i) calcineurin inhibitor (CNI) suppressedT-independent B cell proliferation through B cell receptor, but not T-dependent B cellproliferation through CD40-40L pathway, (ii) anti-metabolite and mTOR inhibitor (mTOR-I)suppressed T-dependent B cell differentiation, (iii) CNI, antimetabolite and mTOR-I suppressed T cell proliferation, (iv) renal transplant recipients with high sensitivityof CSA to T cell demonstrated high prevalence of viral reactivation, and (v) recipientswith low sens tivity exhibited high incidence of acute T cell mediated rejection.It was suggested that PD analysis of B cell and T cell might make it possible to selectthe optimum drug combination for transplant recipients, leading to individualizedimmunosuppression for prevention of chronic rejection.
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