| Project/Area Number |
22590196
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
MAEDA Seishi 兵庫医科大学, 医学部, 准教授 (10309445)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Tetsu 兵庫医科大学, 医学部, 教授(2012 年より連携研究者) (10098543)
OTANI Sachi (KUWAHARA Sachi) 兵庫医科大学, 医学部, 助教(2012 年より連携研究者) (40412001)
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| Research Collaborator |
JUN Jin-gom 兵庫医科大学, 医学研究科, 大学院生
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 腎臓 / アセチルコリン / ネフロン / 自律神経 / 尿細管 / 集合管 / ムスカリン受容体 / 腎上皮 / カリウムチャンネル / 神経 / 腎上皮細胞 / 副交感神経 / 神経節 |
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| Research Abstract |
To clarify the source of acetylcholine (ACh) which acts to the kidney, parasympathetic innervations and non-neuronal expression of choline acetyl transferase (ChAT) were examined in the rat kidney by using a neuronal tracing method and histochemical methods, respectively. Retrograde neuronal tracer Fluoro-gold was treated to the renal nerve ends by a micro-capsule, resulting that there were no positive cells in any parasympathetic nuclei in the medulla oblongata and spinal cords. On the other hand, ACh-synthesizing enzyme ChAT was localized to the renal cortical collecting ducts with uneven distributions in the tubules. These results suggest that the non-neuronal ACh may be predominant over the neuronal ACh in the rat kidney.
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