| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Research Abstract |
The slowly activating delayed rectifier K^+ channel (IKs channel) is composed of KCNQ1 and KCNE1 subunits. The present study was designed to investigate whether activation of Gq protein-coupled receptor (GqPCR) caused endocytosis of the I_<Ks> channel.
1. Effects of GqPCR activation on localization of the pore-forming subunit KCNQ1 were investigated by confocal laser scanning microscopy. It was suggested that AT_1R activation resulted in endocytosis of KCNQ1.
2. AT_1R-induced endocytosis of KCNQ1 was clathrin-dependent, where PKC activation was involved. Ubiquitination of KCNQ1 seemed to have nothing to do with the endocytosis.
3. Activation of α_<1A> and α_<1B> receptor obviously caused endocytosis of KCNQ1. Although AR_<α1>-induced endocytosis of KCNQ1 was clathrin-dependent, it did not require PKC activation in contrast to AT_1R. Involvement of ubiquitination of KCNQ1 was suggested.
|
