| Project/Area Number |
22590245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HAYASHI Takashi 札幌医科大学, 医学部, 助教 (90457698)
KUNO Atsushi 札幌医科大学, 医学部, 助教 (30468079)
KUNIMOTO Risa 札幌医科大学, 医学部, 助教 (20468094)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | サーチュイン / 長寿遺伝子 / 細胞移動 / SIRT1 / neurite / lamellipodia / 神経細胞 / メラノーマ細胞 / PIP3 / Notch / deacetylase / 神経堤 / メラノーマ / p300 / ユビキチン化 / 分化 / NAD / resveratrol / PC12 / NGF |
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| Research Abstract |
We found that SIRT1, an homologue of yeast longevity gene Sir2, bound and deacetylated p300, a co-activator for Notch-intracellular domain, resulting in the ubiquitination and degradation of p300. We found that SIRT1 promoted neurite formation and extension ofPC12 pheochromocytoma cells. We also found that SIRT1 was necessary for lamellipodium formation and migration of B16F1 melanoma cells. Because melanoma cells are derived from neural crest cells, similar mechanism may regulate neural cell migration.
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