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Cellular and microenvironmental factors controlling hepatitis Cvirus infection, examined by micro-proteomics of human liver tissues.

Research Project

Project/Area Number 22590350
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionNihon University

Principal Investigator

ESUMI Mariko  日本大学, 医学部, 准教授 (10147019)

Co-Investigator(Kenkyū-buntansha) SUGITANI Masahiko  日本大学, 医学部, 教授 (40187654)
Research Collaborator YAMAGUCHI Hiromi  日本大学, 医学部, 専修研究員 (90547118)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsC 型肝炎ウイルス / 感染制御 / 微小環境 / レーザーマイクロダイセクション / プロテオミクス / 細胞膜セリンプロテアーゼ / ゴルジ体 / C型肝炎ウイルス / ウイルス量 / GOLM1 / 細胞膜セリンプロテーゼ / TMPRSS2 / ブロテオミクス / 肝非実質
Research Abstract

Hepatitis C virus (HCV) is a major cause of hepatocellular carcinoma. Anti-HCV drugs developed have still some problems such as side effects and drug-resistant mutant viruses. In this study, we investigated a new strategy of humane anti-HCV therapy based on human-made anti-HCV response. We examined which proteins were differentially produced in liver tissues containing different HCV loads by 1000 folds. When we compared them, we obtained liver tissue samples separately from liver parenchyma and stroma by laser microdissection. Ninety and 27 were differential proteins between the two groups of high and low viral loads, from the liver parenchyma and stroma, respectively. Both pro-viral and anti-viral proteins were up-regulated in the high HCV groups. Proteins which function was unknown in the viral infection were examined by the in vitro HCV infection system using cultured cells. We found two proteins up-regulated in the high HCV groups: a serine protease involved in the entry step of HCV infection and a golgi protein related to the secretion of HCV.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (20 results)

All 2013 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (16 results)

  • [Journal Article] β-Glucuronidase is a suitable internal control gene for mRNA quantitation in pathophysiological and non-pathological livers.2013

    • Author(s)
      Hiromi Yamaguchi, Masahiko Sugitani, Mariko Esumi
    • Journal Title

      Experimental and MolecularPathology

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein from the fraction remaining after RNA extraction is useful for proteomics but care must be exercised in its application.2013

    • Author(s)
      Hiromi Yamaguchi, Kiyoshi Hasegawa,Mariko Esumi
    • Journal Title

      Experimental andMolecular Pathology

      Volume: 95 Pages: 46-50

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] High TSC22D3 and low GBP1 expression in the liver is a risk factor for early recurrence of hepatocellular carcinoma2011

    • Author(s)
      Takagi K, Takayama T, Nagase H, Moriguchi M, Wang X, Hirayamagi K, Suzuki T, Hasegawa H, Ochiai T, Yamaguchi N, Kochi M, Kimura M, Esumi M
    • Journal Title

      Experimental and Therapeutic Medicine

      Volume: 2 Pages: 425-431

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] プロテオームとプロテオミクス2010

    • Author(s)
      間世田優文、江角真理子
    • Journal Title

      脊椎脊髄ジャーナル

      Volume: 23 Pages: 553-555

    • Related Report
      2010 Annual Research Report
  • [Presentation] 組織間質からみた肝炎の分子病理:レーザーマイクロダイセクション組織検体の比較プロテオミクスで捉えた肝臓の間質.2012

    • Author(s)
      山口裕美、杉谷雅彦、江角真理子
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞膜セリンプロテアーゼがC型肝炎ウイルス感染感受性に関与する.2012

    • Author(s)
      江角真理子、山口裕美、脇田隆字
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2012-11-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Cellsurface serine protease is involved in the hepatitis Cvirus infection.2012

    • Author(s)
      Mariko Esumi, Hiromi Yamaguchi, Takaji Wakita
    • Organizer
      19th International Symposium in Hepatitis C Virus and Related Viruses.
    • Place of Presentation
      Venice, Italy
    • Year and Date
      2012-10-06
    • Related Report
      2012 Final Research Report
  • [Presentation] C型肝炎ウイルス感染量が異なるヒト非癌部慢性肝炎肝組織で、実質および間質のプロテオミクスから何がわかるか?2012

    • Author(s)
      山口裕美、杉谷雅彦、江角真理子
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      2012-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] C型肝炎ウイルス感染量が異なるヒト非癌部慢性肝炎肝組織で、実質および間質のプロテオミクスから何がわかるか?2012

    • Author(s)
      山口裕美
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cell surface serine protease is involved in the hepatitis C virus infection2012

    • Author(s)
      Mariko Esumi
    • Organizer
      19th International Symposium on Hepatitis C Virus and Related Viruses
    • Place of Presentation
      Venice
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細胞膜セリンプロテアーゼがC型肝炎ウイルス感染感受性に関与する2012

    • Author(s)
      江角真理子
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] Transmembrane protease, serine 2 (TMPRSS2)はC型肝炎ウイルス感染に関与する2012

    • Author(s)
      田井雄飛
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 組織間質からみた肝炎の分子病理:レーザーマイクロダイセクション組織検体の比較プロテオミクスで捉えた肝臓の間質2012

    • Author(s)
      山口裕美
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細胞膜トリプシン様セリンプロテアーゼはC型肝炎ウイルス感染に関与するか?2011

    • Author(s)
      中島聡美、山口裕美、石橋真理子、脇田隆字、江角眞理子
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞膜トリプシン様セリンプロテアーゼはC型肝炎ウイルス感染に関与するか?2011

    • Author(s)
      中島聡美、山口裕美、江角眞理子
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 肝細胞癌の早期再発と微小環境-細胆管増生を伴う非癌部間質のプロテオーム解析2011

    • Author(s)
      山口裕美、黒田和道、宗像康明、尾花ゆかり、高橋理恵、渕之上史、杉谷雅彦、藤原恭子、中山壽之、高山忠利、江角眞理子
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Serumand trypsin inhibitors inhibit the early step of hepatitis C virus infection2011

    • Author(s)
      Mariko Esumi, Hiromi Yamaguchi,Takaji Wakita
    • Organizer
      18th International Symposium on Hepatitis C Virus and Related Viruses.
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2011-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Serum and trypsin inhibitors inhibit the early step of hepatitis C virus infection2011

    • Author(s)
      Esumi M, Kikuta S, Yamaguchi H, Nakajima S, Ishibashi M, Wakita T
    • Organizer
      18^<th> International Symposium on Hepatitis C Virus and Related Viruses
    • Place of Presentation
      シェラトンホテルシアトル(米国)
    • Year and Date
      2011-09-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Liver microenvironment containing cytokeratin 19-positive cells associated with the early recurrence of hepatocellular carcinoma2011

    • Author(s)
      Esumi M, Yamaguchi H, Kuroda K, Munekata Y, Obana Y, Takahashi R, Fuchinoue F, Sugitani M, Nagase H, Takemura T, Minowa T, Hanagata N, Nakayama H, Takayama T
    • Organizer
      The 3rd JCA-AACR Special Joint Conference-The Latest Advances in Liver Cancer Research : From Basic Science to Therapeutics
    • Place of Presentation
      舞浜
    • Year and Date
      2011-03-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] C型肝炎ウイルス感染に対する自然防御について2010

    • Author(s)
      江角眞理子、石橋真理子、鶴田浩一、山口裕美、菊田幸子、榊原由子、脇田隆字
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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