Detection of HIV-1 superinfection by using cluster-specific PCR primers
| Project/Area Number |
22590573
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
MORI Haruyo 大阪府立公衆衛生研究所, 感染症部, 主任研究員 (20250300)
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| Co-Investigator(Kenkyū-buntansha) |
小島 洋子 大阪府立公衆衛生研究所, 感染症部, 主任研究員 (70291218)
川畑 拓也 大阪府立公衆衛生研究所, 感染症部, 主任研究員 (80270768)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | HIV-1 / superinfection / 重複感染 / 特異プライマー / HIV |
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| Research Abstract |
In order to detect HIV-1 superinfection (or dual-infection), we constructed three sets of primers that were designed to specifically amplify HIV-1 strains forming large clusters in Osaka. Proviral DNA samples obtained from 63 HIV-1-infected patients were analysed by nested PCR using the cluster-specific primers, and two cases were found to be dually infected with two distinct HIV-1 subtype B.
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Report
(4 results)
Research Products
(18 results)
