| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
Using endoscopic samples obtained from esophageal squamous cell carcinoma (ESCC) tissues and the surrounding non-tumor tissues, we quantitated microRNA (miR) that were identified via microarray. The expression levels of miR-1246, miR-1290, miR-196a, miR-196b, miR-1914, miR-424, miR-130b, miR-7, and miR-455-3p were elevated more than 2-fold, while miR-203 expression levels were down-regulated more than 2-fold. The miR-1914 expression levels were altered more than 2-fold before and after chemoradiotherapy, and might be specific for ESCC-chemoradiation. Achalasia is considered as the high-risk condition for ESCC, and we explored the miR expression profile in esophageal mucosa of achalasia patients compared to normal controls. It was found that 12 miRs were significantly altered; 7 were elevated and 5 were down-regulated. Of significance, miR-21 expression was significantly elevated in the non-ESCC samples from achalasia patients. Moreover, the target molecules were down-regulated, suggesting the function roles of miR-21. On the other hand, the expression of miR-1246, miR-196a, miR-196b, miR-1914, miR-424, miR-130b, miR-7, and miR-455-3p was not significant, implying the ESCC specific miR, albeit the blood levels were not substantial. The circulating miR-1290 was found to be elevated in the studies, and it is warrant to be explored as the ESCC-specific miR.
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