Clinical significance of esophageal squamous cell carcinoma-specific microRNA in circulation and tissue
| Project/Area Number |
22590685
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | マイクロRNA / 食道扁平上皮癌 / 内視鏡的粘膜下層剥離術 / 化学放射線療法 / 逆流性食道炎 / アカラシア / パピローマウイルス / エキソソーム / バレット食道・腺癌 |
|---|
| Research Abstract |
Using endoscopic samples obtained from esophageal squamous cell carcinoma (ESCC) tissues and the surrounding non-tumor tissues, we quantitated microRNA (miR) that were identified via microarray. The expression levels of miR-1246, miR-1290, miR-196a, miR-196b, miR-1914, miR-424, miR-130b, miR-7, and miR-455-3p were elevated more than 2-fold, while miR-203 expression levels were down-regulated more than 2-fold. The miR-1914 expression levels were altered more than 2-fold before and after chemoradiotherapy, and might be specific for ESCC-chemoradiation. Achalasia is considered as the high-risk condition for ESCC, and we explored the miR expression profile in esophageal mucosa of achalasia patients compared to normal controls. It was found that 12 miRs were significantly altered; 7 were elevated and 5 were down-regulated. Of significance, miR-21 expression was significantly elevated in the non-ESCC samples from achalasia patients. Moreover, the target molecules were down-regulated, suggesting the function roles of miR-21. On the other hand, the expression of miR-1246, miR-196a, miR-196b, miR-1914, miR-424, miR-130b, miR-7, and miR-455-3p was not significant, implying the ESCC specific miR, albeit the blood levels were not substantial. The circulating miR-1290 was found to be elevated in the studies, and it is warrant to be explored as the ESCC-specific miR.
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Report
(4 results)
Research Products
(2 results)
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[Presentation] 食道癌の診断2011
Author(s)
南 ひとみ, 磯本 一, 中山敏幸, 松島加代子, 赤澤祐子, 山口直之, 大仁田賢, 竹島史直, 宿輪三郎, 林 徳眞吉, 中尾一彦
Organizer
第92回日本消化器内視鏡学会九州支部例会
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