| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
To clarify dynamic mechanism of cardiac dendritic cell (DC) is the present purpose. Firstly, I established a novel GFP labeled T cell line specific reactive to cardiac myosin (CMT) using CM2 peptide, and analyzed DC activationby the transfer experiment. As a result, persistence of autoimmune myocarditis and change ofT cell subsets were seen. The GFP-CMT infiltrated into the myocardium directly and induced inflammation. In addition, Th17 T cells appeared in vicinity of the lesions followingafter disappearance of GFP-CMT. This phenomenon was an alternation of T-cell. The DC played an important role in the process. For DC cell kinetics, I investigated the transfer myocarditis in GFP overexpressed rat employing non GFP-labeled CMT, and tried to detect GFP-positive DC. Then, I isolated GFP-negative CD3-positive cells and tested them by Flowcytometry. However, I consumed much time to create their chimera models, and so failed to obtain sufficient results. IL-23/27 balance, Th17 immunity, GFP-positive DC mobilization and its TLR signaling emerged as challengeable tasks. On the other hand, Cylindromatosis (CYLD) is an activator of natural immunity and an inhibitor of NF-κB. Expression of this CYLD has been still unclear. In the present search on activated macrophage, it is suggested that Interferon regulatory factor (IRF)-3 is extensively involved in its expression. From a systematic analysis, to provoke myocarditis, to prolong it andto fall into fulminant type, this signal seems to be essential. Currently, DC kinetics in this signaling becomes a new theme.
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