| Project/Area Number |
22591110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
KAKEYA Hirosi 長崎大学, 医歯薬学総合研究科, 准教授 (40398152)
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| Co-Investigator(Kenkyū-buntansha) |
KOUNO Sigeru 長崎大学, 医歯薬学総合研究科, 教授 (80136647)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 接着菌 / シグナルシークエンス / 診断キット / 接合菌 |
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| Research Abstract |
The diagnosis of mucormycosis relies upon the identification of organisms in tissue by histopathology with culture confirmation. However, culture often yields no growth, and histopathologic identification of an organism with a structure typical of Mucorales may provide the only evidence of infection. A clinician must think of this entity in the appropriate clinical setting and pursue invasive testing in order to establish a diagnosis as early as possible. Therefore, new early detection methods are required. We used a novel signal sequence trap methods to select protein antigen from Rhizopus oryzae. Two unknown proteins with signal sequences, antigen A (26kDa, hypothetical protein) and B (46kDa, hypothetical protein) were selected as candidates. We made ELISA kit to detect antigen A. The antigen A was detected in the culture supernatant of R. oryzae、R microspores、R microspores var. rhizopodiformis. The antigen was also detected in the serum form Rhizopus infected mice.
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