Age-related rupture of internal elastic lamina in atherosclerosis: ultrastrucutral analysis of its occurrence and healing.

• Project/Area Number: 22591542
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Okayama University
• Principal Investigator: OHTSUKA Aiji 岡山大学, 大学院・医歯薬学総合研究科, 教授 (50168986)
• Co-Investigator(Kenkyū-buntansha): TAGUCHI Takehito 岡山大学, 大学院・保健学研究科, 教授 (70188134) ; MOMOTA Ryusuke 岡山大学, 大学院・医歯薬学総合研究科, 助教 (80263557)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 弾性線維 / 動脈硬化症 / 内弾性板 / 走査型電子顕微鏡 / 血管鋳型法 / 消化法 / エラスチン / 三次元構造 / 査型電子顕微鏡 / 断裂 / 動脈硬化 / 再生線維 / 組織消化法 / アミノ酸分析 / 血管 / 循環器・高血圧 / 細胞・組織 / 動脈 / 電子顕微鏡 / 血管鋳型標本

Research Abstract

At first, we newly developed scanning electron microscopy technique using a formic acid digestion with vascular casts, which revealed, in detail, the three-dimensional(3D) architecture of delicate elastic fibers in small vessels. This method could successfully visualized the three-dimensional architecture of elastic fibers in small various sized of blood vessels, even arterioles and venules. Furthermore, the new method revealed the 3D architectures of corrupted internal elastic lamina.

Research Products

• [Journal Article] Architecture of the subendothelial elastic fibers of small blood vessels and variations in vascular type and size (2013)
  • Author(s): Shinaoka A, Momota RKosaka M, Kumagishi K, Nakahara R, Naito I, Ohtsuka A
  • Journal Title: Microscopy and Microanalysis Volume: 19(2) Pages: 406-414
  • NAID: 120005359113
  • Peer Reviewed
• [Journal Article] Architecture of the Subendothelial Elastic Fibers of Small Blood Vessels and Variations in Vascular Type and Size (2013)
  • Author(s): Akira Shinaoka
  • Journal Title: Microscopy and Microanalysis Volume: 19 Issue: 2 Pages: 406-414
  • DOI: 10.1017/s1431927612014341
  • Peer Reviewed
• [Presentation] 微小血管の内皮下弾性線維の走査電子顕微鏡観察～血管の種類とサイズに応じての変化 (2012)
  • Author(s): 品岡 玲、百田龍輔、熊岸加苗、小阪美津子、中原龍一、内藤一郎、大塚愛二
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨
  • Year and Date: 2012-03-27
• [Presentation] 微小血管内皮下弾性線維構造の走査電子顕微鏡観察～血管の種類とサイズに応じた変化～ (2012)
  • Author(s): 品岡玲
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-27
• [Presentation] 微小血管内皮下弾性線維構造と血行動態の関係 (2011)
  • Author(s): 品岡 玲、百田龍輔 、小阪美津子、内藤一郎、中原龍一、大塚愛二
  • Organizer: 第43回日本結合組織学会・第58回マトリックス研究会合同学術集会
  • Place of Presentation: 大分
  • Year and Date: 2011-06-11
• [Presentation] 微小血管内皮下弾性線維構造とその血行動態との関係 (2011)
  • Author(s): 品岡玲
  • Organizer: 第43回日本結合組織学会学術大会・第58回マトリックス研究会大会合同学術集会
  • Place of Presentation: 別府ビーコンプラザ(大分県)
  • Year and Date: 2011-06-11
• [Presentation] Three-dimensional architecture of elastic fibers in various blood vessel walls : scanning electron microscopy of resin injected and digested specimen. (2010)
  • Author(s): Shinaoka, A., et al.
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 神奈川県パシフィコ横浜震災で中止,誌上発表
  • Year and Date: 2010-03-29
• [Remarks]
  • URL: http://www.okayama-u.ac.jp/user/anatomy/hm/index.html
• [Remarks]
  • URL: http://www.okayama-u.ac.jp/user/anatomy/hm/