Analyssis of the fate determination mechanism to corneal epithelial cells using fluorescent reporter

• Project/Area Number: 22591966
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Keio University
• Principal Investigator: YOSHIDA Satoru 慶應義塾大学, 医学部, 特任講師 (50398781)
• Co-Investigator(Renkei-kenkyūsha): SHIMMURA Shigeto 慶應義塾大学, 医学部, 准教授 (00235780)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 眼発生 / 再生医学 / 角膜上皮細胞 / ケラチン12 / 角膜上皮 / ケラチン１２ / 幹細胞

Research Abstract

We constructed a K12-DsRed BAC reporter, which express DsRed under the control of Krt12 promoter. The K12-DsRed reporter was introduced into a mouse corneal epithelial cell line, TKE2, and a mouse iPS cell line. An activity that converts bulge stem cells

Research Products

• [Journal Article] Functional corneal endothelium derived from corneal stroma stem cells of neural crest origin by retinoic acid and Wnt/β-catenin signaling. (2013)
  • Author(s): 羽藤晋
  • Journal Title: Stem Cells and Development Volume: 22(5) Issue: 5 Pages: 828-183
  • DOI: 10.1089/scd.2012.0286
  • Peer Reviewed
• [Journal Article] Aquaporin 1-positive stromal niche-like cells directly interact with N-cadherin-positive clusters in the basal limbal epithelium. (2013)
  • Author(s): 比嘉一成
  • Journal Title: Stem Cell Research Volume: 10(2) Issue: 2 Pages: 147-155
  • DOI: 10.1016/j.scr.2012.11.001
  • Peer Reviewed
• [Journal Article] The Semaphorin 3A Inhibitor SM-345431 Accelerates Peripheral Nerve Regeneration and Sensitivity in a Murine Corneal Transplantation Model. (2012)
  • Author(s): 大元雅弘
  • Journal Title: PLoS One Volume: 7(11) Issue: 11 Pages: e47716-e47716
  • DOI: 10.1371/journal.pone.0047716
  • Peer Reviewed
• [Journal Article] Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells (2011)
  • Author(s): Yoshida S, Yasuda M, Miyashita H, Ogawa Y, Yoshida T, Matsuzaki Y, Tsubota K, Okano H, Shimmura S
  • Journal Title: PLoS One Volume: 6(12) Issue: 12 Pages: 1-1
  • DOI: 10.1371/journal.pone.0028856
  • Peer Reviewed
• [Presentation] iPS細胞から誘導した神経堤細胞のケラトサイトへの分化 (2013)
  • Author(s): 吉田 悟
  • Organizer: 角膜カンファランス2013
  • Place of Presentation: 和歌山
• [Presentation] 培養下における角膜輪部ニッチ自己組織化 (2013)
  • Author(s): 宮下英之
  • Organizer: 角膜カンファランス2013
  • Place of Presentation: 和歌山
• [Presentation] Differentiation Of Induced Pluripotent Stem Cell-Derived Neural Crest Cells Into Corneal Keratocytes In Vivo International Society for Stem Cell (2012)
  • Author(s): Yoshida S, Miyashita H, Ohta S, Yasuda M, Inagaki E, Tsubota K, Okano H, Shimmura S.
  • Organizer: 10th Annual Meeting, Yokohama
  • Place of Presentation: Japan
• [Presentation] Differentiation Of Induced Pluripotent Stem Cell-Derived Neural Crest Cells Into Corneal Keratocytes In Vivo (2012)
  • Author(s): 吉田 悟
  • Organizer: International Society for Stem Cell (ISSCR) 10th Annual Meeting
  • Place of Presentation: 横浜
• [Presentation] トランスレーショナル医療による新しい角膜治療法 (2012)
  • Author(s): 羽藤 晋
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] Generation Of Stratified Squamous Epithelial Cells From Induced Pluripotent Stem Cells (2011)
  • Author(s): 吉田悟
  • Organizer: 2011, Association for Research in Vision and Ophthalmology
  • Place of Presentation: フォートローダデール(アメリカ)
  • Year and Date: 2011-05-04
• [Presentation] Generation of stratified squamous epithelial cells from induced pluripotent stem cells (2011)
  • Author(s): Yoshida S, Yasuda M, Miyashita H, Yoshida T, Okano H, Tsubota K, Shimmura S.
  • Organizer: The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
• [Presentation] Differentiation of induced pluripotent stem cells into corneal epithelial cells (2010)
  • Author(s): Yoshida S, Yasuda M, Miyashita H, Yoshida T, Tsubota K, Okano H, Shimmura S
  • Organizer: The 16th International Conference of the International Society of Differentiation
  • Place of Presentation: Nara, Japan
• [Presentation] Differentiation of induced pluripotent stem cells into corneal epithelial cells. (2010)
  • Author(s): Yoshida S, Shimmura S, Okano H, Tsubota K.
  • Organizer: The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Fort Lauderdale, FL, USA
• [Book] iPS 細胞からの角膜上皮誘導. 臨床眼科増刊 オキュラーサーフェス診療アップデート (2012)
  • Author(s): 羽藤晋, 吉田悟, 榛村重人
  • Publisher: 医学書院