Establishment of periodontal ligament (PDL) cells derived from deciduous teeth and periodontal tissue regeneration with PDL cells.

• Project/Area Number: 22592296
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: The University of Tokushima (2011-2012); Iwate Medical University (2010)
• Principal Investigator: HASEGAWA Tomokazu 徳島大学, 病院, 講師 (50274668)
• Co-Investigator(Kenkyū-buntansha): ISHISAKI Akira 岩手医科大学, 歯学部, 教授 (20356439)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 小児歯科学 / 乳歯 / 歯根膜 / 株化 / hTERT / 再生医学 / SDF-1

Research Abstract

We established clonal cell lines of periodontal ligament cells derived from human deciduous teeth immortalized by hTERT gene transfer. SH 9 cells showed the ability of differentiation into both osteoblasts and adipocytes. Stromal cell-derived factor-1α(SDF-1) has important roles in migration of mesenchymal stem cells and endothelial progenitor cells. We reported that SDF-1 expression in SH 9 cells was regulated by various cytokines.

Research Products

• [Journal Article] Forced mastication increases survival of adult neural stem cells in the hippocampal dentate gyrus. (2013)
  • Author(s): Yuki Akazawa, Takamasa Kitamura, Yuri Fujihara, Yoshitaka Yoshimura, Masato Mitome and Tomokazu Hasegawa
  • Journal Title: Int J Mol Med Volume: 31 Issue: 2 Pages: 307-314
  • DOI: 10.3892/ijmm.2012.1217
  • Peer Reviewed
• [Journal Article] TGF-β-operated growth inhibition and translineage commitment into smooth muscle cells of periodontal ligamentderived endothelial progenitor cells through Smad- and p38 MAPK dependentsignals (2012)
  • Author(s): Yoshida M, Okubo N, Chosa N, Hasegawa T, Ibi M, Kamo M, Kyakumoto S, & Ishisaki A
  • Journal Title: Int. J. Biol. Sci. Volume: 8 Issue: 7 Pages: 1062-1074
  • DOI: 10.7150/ijbs.4488
  • Peer Reviewed
• [Journal Article] Effects of bisphosphonates on osteoclastogenesis in RAW264.7 cells (2012)
  • Author(s): Abe K, Yoshimura Y, Deyama Y, et al
  • Journal Title: International Journal of Molecular Medicine Volume: 29 Pages: 1007-1015
  • DOI: 10.3892/ijmm.2012.952
  • Peer Reviewed
• [Journal Article] Differential effects of TGF-β1 and FGF-2 on SDF-1αexpression in human periodontal ligament cells derived from deciduous tooth in vitro. (2012)
  • Author(s): Tomokazu Hasegawa, Naoyuki Chosa,Takeyoshi Asakawa, Yoshitaka Yoshimura, Yuri Fujihara, Takamasa Kitamura, Mitsuro Tanaka, Akira Ishisaki and Masato Mitome
  • Journal Title: Int J Mol Med Volume: 30 (1) Pages: 35-40
  • DOI: 10.3892/ijmm.2012.957
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1 α in periodontal ligament cells derived from human permanent teeth in vitro (2012)
  • Author(s): Asakawa T
  • Journal Title: International Journal of Molecular Medicine Volume: 29 Issue: 4 Pages: 569-573
  • DOI: 10.3892/ijmm.2011.869
  • Peer Reviewed
• [Journal Article] 乳歯歯根膜細胞による再生医学研究の可能性の検討 (2012)
  • Author(s): 長谷川智一
  • Journal Title: 小児歯誌 Volume: 50 (1) Pages: 7-14
  • NAID: 10030558370
  • URL: http://www.meteo-intergate.com/linkout/dlview.php?ac=9&cn=1&ar=/du8shoni/2012/005001/002/0007-0014&us=118134&dt=2013-04-0410:41:44&hs=0dc1a68ad50475b80559f463b07986ee34ef29af
• [Journal Article] Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1α in periodontal ligament cells derived from human (2012)
  • Author(s): Asakawa T, et al
  • Journal Title: Int J Mol Med Volume: (in press)
  • Peer Reviewed
• [Journal Article] Differential effects of TGF-β1 and FGF-2 on SDF-1α expression in human periodontal ligament cells derived from deciduous tooth in vitro (2012)
  • Author(s): Hasegawa T, et al
  • Journal Title: Int J Mol Med Volume: (in press)
  • Peer Reviewed
• [Journal Article] Stage-dependent suppression of the formation of dentin-resorbing multinuclear cells with MIF in vitro (2011)
  • Author(s): Kikuiri T, Yoshimura Y, Tabata F, Hasegawa T, Nishihira J, Shirakawa T
  • Journal Title: Experimental and Therapeutic Medicine Volume: 3 Issue: 1 Pages: 37-43
  • DOI: 10.3892/etm.2011.362
  • Peer Reviewed
• [Journal Article] Dental pulp cells derived from permanent teeth express higher levels of R-cadher in than do deciduous teeth : Implications of the correlation between R-cadherin expression and restriction of multipotency in mesenchymal stem cells (2011)
  • Author(s): Takahashi N
  • Journal Title: Archives of Oral Biology Volume: 57 Issue: 1 Pages: 44-51
  • DOI: 10.1016/j.archoralbio.2011.07.013
  • Peer Reviewed
• [Journal Article] Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells (2011)
  • Author(s): Nomura M, Yoshimura Y, Kikuiri T, Hasegawa T, Taniguchi Y, Deyama Y, Koshiro K, Sano H, Suzuki K, Inoue N.
  • Journal Title: Journal of Pharmacological Sciences Volume: 117 Issue: 4 Pages: 243-252
  • DOI: 10.1254/jphs.11099FP
  • NAID: 10030453660
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Effect of the release from mechanical stress on osteoclastogenesis in RAW 264. 7 cells (2011)
  • Author(s): Shibata K, Yoshimura Y, Kikuiri T, Hasegawa T, Taniguchi Y, Deyama Y, Suzuki K, Iida J.
  • Journal Title: Int J Molecular Medicin Volume: 28 Pages: 73-79
  • DOI: 10.3892/ijmm.2011.675
  • Peer Reviewed
• [Journal Article] Establishment of immortalized human periodontal ligament cells derived from deciduous teeth. (2010)
  • Author(s): Tomokazu Hasegawa, Naoyuki Chosa, Takeyoshi Asakawa, Yoshitaka Yoshimura, Akira Ishisaki and Mitsuro Tanaka.
  • Journal Title: Int J Mol Med Volume: 26(5) Issue: 5 Pages: 701-705
  • DOI: 10.3892/ijmm_00000516
  • Peer Reviewed
• [Journal Article] Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro (2010)
  • Author(s): Tomokazu Hasegawa, Naoyuki Chosa, Takeyoshi Asakawa, Yoshitaka Yoshimura, Asami Asakawa, Akira Ishisaki and Mitsuro Tanaka
  • Journal Title: Exp Ther Med Volume: 1(3) Issue: 3 Pages: 477-477
  • DOI: 10.3892/etm_00000074
  • Peer Reviewed
• [Journal Article] Effect of fibroblast growth factor-2 on periodontal ligament cells derived from human deciduous teeth in vitro. (2010)
  • Author(s): Tomokazu Hasegawa, Naoyuki Chosa, Takeyoshi Asakawa, Yoshitaka Yoshimura, Asami Asakawa, Akira Ishisaki and Mitsuro Tanaka
  • Journal Title: Exp Ther Med Volume: 1(2) Issue: 2 Pages: 337-337
  • DOI: 10.3892/etm_00000052
  • Peer Reviewed
• [Journal Article] Effect of fibroblast growth factor-2 on periodontal ligament cells derived from human deciduous teeth in vitro. (2010)
  • Author(s): Tomokazu Hasegawa
  • Journal Title: Exp Ther Med Volume: 1 Pages: 337-341
  • Peer Reviewed
• [Journal Article] Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro. (2010)
  • Author(s): Tomokazu Hasegawa
  • Journal Title: Exp Ther Med Volume: 1 Pages: 477-480
  • Peer Reviewed
• [Journal Article] Establishment of immortalized human periodontal ligament cells derived from deciduous teeth. (2010)
  • Author(s): Tomokazu Hasegawa
  • Journal Title: Int J Mol Med Volume: 26 Pages: 701-705
  • Peer Reviewed
• [Presentation] 乳歯歯根膜はSDF-1αにより間葉系幹細胞の遊走をコントロールし (2012)
  • Author(s): 長谷川智一、帖佐直幸、藤原百合、北村尚正、浅川剛吉、赤澤友基、吉村善隆、石崎明、三留雅人
  • Organizer: 歯周組織の再生・恒常性に関与している. 第50回日本小児歯科学会大会
  • Place of Presentation: 東京国際フォーラム(東京都)
• [Presentation] 乳歯歯根膜はSDF-1αにより間葉系幹細胞の遊走をコントロールし、歯周組織の再生・恒常性に関与している (2012)
  • Author(s): 長谷川智一
  • Organizer: 第50回日本小児歯科学会大会
  • Place of Presentation: 東京国際フォーラム（東京都）
• [Presentation] ヒト乳歯歯根膜細胞におけるFGF-2 およびTGF-β1によるSDF-1発現への影響. (2011)
  • Author(s): 長谷川智一, 帖佐直幸, 浅川剛吉,浅川麻美,吉村善隆,石崎 明, 田中光郎
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: いわて県民情報交流センター(盛岡市)
  • Year and Date: 2011-11-28
• [Presentation] ヒト永久歯歯根膜細胞におけるSDF-1発現調節の解析 (2011)
  • Author(s): 浅川剛吉, 帖佐直幸, 長谷川智一, 浅川麻美, 石崎 明, 田中光郎
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: いわて県民情報交流センター(盛岡市)
  • Year and Date: 2011-11-28
• [Presentation] ヒト永久歯歯根膜細胞におけるSDF-1発現調節の解析 (2011)
  • Author(s): 浅川剛吉
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: 盛岡市(いちて県民情報交流センターアイーナ)
  • Year and Date: 2011-11-28
• [Presentation] ヒト乳歯歯根膜細胞におけるFGF-2およびTGF-β1によるSDF-1発現への影響 (2011)
  • Author(s): 長谷川智一
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: 盛岡市(いちて県民情報交流センターアイーナ)
  • Year and Date: 2011-11-28
• [Presentation] 乳歯歯根膜細胞による再生医学研究の可能性の検討 (2011)
  • Author(s): 長谷川智一
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: 盛岡市(いちて県民情報交流センターアイーナ)(受賞講演)
  • Year and Date: 2011-11-28
• [Presentation] 歯周靭帯由来線維芽細胞の増殖と平滑筋分化はFGFで誘導されるERKシグナルにより制御される (2011)
  • Author(s): 高橋美香子
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜市(長良川国際会議場)
  • Year and Date: 2011-10-02
• [Presentation] 歯周靭帯由来細胞の血管構成細胞分化におけるTGF-βの関与について (2011)
  • Author(s): 吉田茉莉子
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜市(長良川国際会議場)
  • Year and Date: 2011-10-01
• [Presentation] 日本小児歯科学会学術賞 Lion Award 学術賞受賞講演: 乳歯歯根膜細胞による再生医学研究の可能性の検討. (2011)
  • Author(s): 長谷川智一
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: いわて県民情報交流センター(盛岡市)
• [Presentation] Eatablishment of periodontal ligament cell line derived from human deciduous teeth. (2011)
  • Author(s): Tomokazu Hasegawa, Naoyuki Chosa, Takeyoshi Asakawa, Yoshitaka Yoshimura, Akira Ishisaki and Mitsuro Tanaka
  • Organizer: The 4th international symposium for interface oral health science in sendai
  • Place of Presentation: ホテル仙台プラザ (仙台市)
• [Presentation] Mituro Tanaka. Regulation of SDF-1 expression in periodontal ligament cells derived from human permanent teeth. (2011)
  • Author(s): Takeyoshi Asakawa, Naoyuki Chosa, Tomokazu Hasegawa, Asami Asakawa, Akira Isisaki
  • Organizer: The 4th international symposium for interface oral health science in sendai.
  • Place of Presentation: ホテル仙台プラザ(仙台市)
• [Presentation] ヒト永久歯歯根膜細胞におけるSDF-1の発現 (2010)
  • Author(s): 浅川剛吉, 帖佐直幸, 長谷川智一, 浅川麻美, 石崎 明, 田中光郎
  • Place of Presentation: 郡山市民文化センター(郡山市)
  • Year and Date: 2010-12-03
• [Presentation] ヒト乳歯歯根膜細胞の細胞株樹立. (2010)
  • Author(s): 長谷川智一, 帖佐直幸, 浅川剛吉,吉村善隆,浅川麻美, 石崎 明, 田中光郎
  • Organizer: 平成22年度日本小児歯科学会秋季大会
  • Place of Presentation: 郡山市民文化センター(郡山市)
• [Presentation] PDGF受容体を介したVCAM1の発現は間葉系幹細胞の遊走を抑制する. (2010)
  • Author(s): 西平宗功, 大久保直登, 高橋典子, 長谷川智一, 帖佐直幸, 杉山芳樹, 石崎 明
  • Organizer: 第52回歯科基礎医学会学術大会・総会.
  • Place of Presentation: タワーホール船堀(東京都)
• [Presentation] ヒト乳歯歯根膜細胞におけるSDF-1の発現調節機構 (2010)
  • Author(s): 長谷川智一, 帖佐直幸, 西平宗功, 石崎 明, 田中光郎
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: タワーホール船堀(東京都)
• [Presentation] ヒト乳歯歯根膜由来線維芽細胞様細胞におけるSDF-1の発現 (2010)
  • Author(s): 長谷川智一、帖佐直幸、浅川剛吉、浅川麻美、吉村善隆、石崎 明、田中光郎
  • Organizer: 第48回日本小児歯科学会大会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
• [Presentation] ヒト乳歯歯根膜由来線維芽細胞様細胞におけるSDF-1の発現 (2010)
  • Author(s): 長谷川智一
  • Organizer: 第48回日本小児歯科学会大会
  • Place of Presentation: 名古屋
• [Presentation] 帖佐直幸: 間葉系幹細胞におけるPDGFレセプターを介したVCAM1の発現解析. (2009)
  • Author(s): 西平宗功, 大久保直登, 高橋典子, 客本斉子, 加茂政晴, 長谷川智一, 杉山芳樹, 石崎 明
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸コンベンションセンター(神戸市)
• [Book] Eatablishment of periodontal ligament cell line derived from human deciduous teeth.In: Interface oral health science 2011 (2012)
  • Author(s): Tomokazu Hasegawa, Naoyuki Chosa, Takeyoshi Asakawa, Yoshitaka Yoshimura, Akira Ishisaki and Mitsuro Tanaka
  • Publisher: Sasaki, K. (ed.) Springer, New York
• [Book] Regulation of SDF-1 expression in periodontal ligament cells derived from human permanent teeth. In: Interface oral health science 2011 (2012)
  • Author(s): Takeyoshi Asakawa, Naoyuki Chosa, Tomokazu Hasegawa, Asami Asakawa, Akira Isisaki, Mituro Tanaka
  • Publisher: Sasaki, K. (ed.) Springer, New York
• [Book] Interface oral health science 2011 (2012)
  • Author(s): Hasegawa T
  • Publisher: Springer
• [Book] Interface oral health science 2011 (2012)
  • Author(s): Asakawa T
  • Publisher: Springer
• [Book] Regulation of SDF-1 expression in periodontal ligament cells derived from human permanent teeth (2012)
  • Author(s): Asakawa T, et al
  • Total Pages: 3
  • Publisher: Springer
• [Book] Eatablishment of periodontal ligament cell line derived from human deciduous teeth (2012)
  • Author(s): Hasegawa T, et al
  • Total Pages: 3
  • Publisher: Springer
• [Book] 乳歯歯根膜細胞による再生医学研究の可能性の検討 (2012)
  • Author(s): 長谷川智一
  • Publisher: 株式会社二紀出版(in press)
• [Remarks]
  • URL: http://dent-open.iwate-med.ac.jp/soshiki/member23.html