Development of reverse-genetics methods for Daphnids

• Project/Area Number: 22651078
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied genomics
• Research Institution: National Institutes of Natural Sciences Okazaki Research Facilities
• Principal Investigator: IGUCHI Taisen 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90128588)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: ミジンコ / 逆遺伝学 / エコゲノミクス

Research Abstract

The water flea Daphnia magna is a small crustacean which is an ubiquitous inhabitant in fresh water. As daphnids inhabit a diverse array of aquatic environments, they are a good model for understanding response and adaptation to environmental changes and evolution. Therefore, they have been used one of standard organisms in ecotoxicology. While daphnids have significant importance in ecotoxicology, tools to understand response to environmental changes in daphnia is very limited. We investigate a condition for natural male production and improve introduction of foreign DNA into daphnids. These basic information will be available for establishment of transgenic daphnids.

Research Products

• [Journal Article] Environmental sex determination in the branchiopod crustacean Daphnia magna : Deep conservation of a Doublesex gene in the sex-determining pathway (2011)
  • Author(s): Kato, Y., Kobayashi, K., Watanabe, H. and Iguchi, T
  • Journal Title: PLoS Genetics Volume: 7
  • NAID: 120004837638
  • Peer Reviewed
• [Journal Article] Development of a RNA interference method in the cladoceran crustacean Daphnia magna (2011)
  • Author(s): Kato, Y., Shiga, Y., Kobayashi, K., Tokishita, S., Yamagata, H., Iguchi, T. and Watanabe, H
  • Journal Title: Devel. Genes Evol Volume: 220 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Morphological changes in Daphnia galeata induced by a crustacean terpenoid hormone and its analog (2011)
  • Author(s): Oda, S., Kato, Y., Watanabe, H., Tatarazako, N., Iguchi, T
  • Journal Title: Environ Toxicol Chem Volume: 30 Pages: 232-238
  • Peer Reviewed
• [Journal Article] Demasculinization and feminization of male gonads by atrazine : consistent effects across vertebrate classes (2011)
  • Author(s): Hayes, T. B., Beasley, V. R., de Solla, S., Iguchi, T., et al
  • Journal Title: J. Steroid Biochem Mol. Biol Volume: 127 Pages: 64-73
  • Peer Reviewed
• [Journal Article] Endocrine disrupting chemicals. J. Steroid Biochem (2011)
  • Author(s): Blumberg, B., Iguchi, T. and Odermatt, A
  • Journal Title: Mol. Biol Volume: 127 Pages: 1-3
• [Journal Article] Species extrapolation for the 21st century. Environ (2011)
  • Author(s): Celander, M. C., Goldstone, J. V., Denslow, N. D., Iguchi, T., Kille, P., Meyerhoff, R. D., Smith, B. A., Hutchinson T. H. and Wheeler, J. R
  • Journal Title: Toxicol. Chem Volume: 30 Pages: 5263-5263
  • Peer Reviewed
• [Journal Article] Endocrine disrupting chemicals (2011)
  • Author(s): Blumberg, B., Iguchi, T., et al
  • Journal Title: J Steroid Biochem Mol Biol Volume: 127 Pages: 1-3
• [Journal Article] Demasculinization and feminization of male gonads by atrazine : consistent effects across vertebrate classes (2011)
  • Author(s): Hayes, T.B., Iguchi, T., et al
  • Journal Title: J Steroid Biochem Mol Biol Volume: 127 Pages: 64-73
  • Peer Reviewed
• [Journal Article] Species extrapolation for the 21st century (2011)
  • Author(s): Celander, M.C, Iguchi, T., et al
  • Journal Title: Environ Toxicol Chem Volume: 30 Pages: 52-63
• [Journal Article] Environmental sex determination in the branchiopod crustacean Daphnia magna : Deep conservation of a Doublesex gene in the sex-determining pathway (2011)
  • Author(s): Kato Y., Iguchi T., et al.
  • Journal Title: PLoS Genetics Volume: 7
  • NAID: 120004837638
  • Peer Reviewed
• [Journal Article] Development of an RNA interference method in the cladoceran crustacean Daphnia magna. (2011)
  • Author(s): Kato Y., Iguchi T., et al.
  • Journal Title: Devel.Genes Evol Volume: 220 Pages: 337-345
  • Peer Reviewed
• [Journal Article] Sequence divergence and expression of a transformer gene in the branchiopod crustacean (2010)
  • Author(s): Kato, Y., Kobayashi, K., Oda, S., Tatarazako, N., Watanabe, H. and Iguchi, T
  • Journal Title: Daphnia magna. Genomics Volume: 95 Pages: 160-165
  • Peer Reviewed
• [Journal Article] Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation (2010)
  • Author(s): Kato, Y., Kobayashi, K., Watanabe, H. and Iguchi, T
  • Journal Title: Ecotoxicology Volume: 19 Pages: 589-592
  • Peer Reviewed
• [Journal Article] Morphological changes in Daphnia galeata induced by a crustacean terpenoid hormone and its analog (2010)
  • Author(s): Oda S., Iguchi T., et al.
  • Journal Title: Environ.Toxicol.Chem Volume: 30 Pages: 232-238
  • Peer Reviewed
• [Presentation] オオミジンコの性分化機構 (2011)
  • Author(s): 井口泰泉
  • Organizer: 琉球大学熱帯生物圏研究センター共同利用研究会「動物の性決定機構」
  • Place of Presentation: 本部町産業支援センター(沖縄県本部町)
  • Year and Date: 2011-02-23
• [Presentation] 内分泌かく乱物質の生物影響に関する研究 (2011)
  • Author(s): 井口泰泉
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 北海道旭川市
• [Presentation] Endocrine disrupters issues in Japan (2011)
  • Author(s): Iguchi, T
  • Organizer: 2nd International Conference on Endocrine Disruptors
  • Place of Presentation: ドイツ
• [Presentation] Endocrine disruption in invertebrates : Sex determination of Daphnia magna (2011)
  • Author(s): Iguchi, T
  • Organizer: 6th Copenhagen Workshop on Endocrine Disrupters
  • Place of Presentation: デンマーク
• [Presentation] オオミジンコのエクジクジステロイド生合成関連遺伝子群の単離及び発現解析 (2011)
  • Author(s): 角谷絵里, 井口泰泉, ら
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道旭川市)
• [Presentation] オオミジンコDaphnia magnaの性決定期における幼若ホルモン応答遺伝子の網羅的解析 (2011)
  • Author(s): 豊田賢治, 井口泰泉, ら
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道旭川市)
• [Presentation] ミジンコDaphnia pulexにおける捕食者カイロモンに応じた胚発生過程の変化 (2011)
  • Author(s): 宮川一志, 井口泰泉, ら
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道旭川市)
• [Presentation] 内分泌かく乱物質の生物影響に関する研究 (2011)
  • Author(s): 井口泰泉, ら
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道旭川市)
• [Presentation] Development of an RNA interference method in the cladoceran Daphnia magna (2011)
  • Author(s): Kato, Y., Iguchi, T., er al
  • Organizer: 分子生物学会第34回大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
• [Presentation] Endocrine disruption in invertebrates : Sex determination of Daphnia magna (2011)
  • Author(s): Iguchi, T.
  • Organizer: 6th Copenhagen Workshop on Endocrine Disrupters
  • Place of Presentation: コペンハーゲン大学病院(デンマーク、コペンハーゲン)
• [Presentation] Endocrine disrupters issues in Japan (2011)
  • Author(s): Iguchi, T., er al
  • Organizer: 2nd International Conference on Endocrine Disruptors
  • Place of Presentation: ホリデーインフランクフルトエアポートノース(ドイツ、フランクフルト)
• [Presentation] Estrogens and sex determination : Cross-species investigations with alligators, medaka, and Daphnia (2011)
  • Author(s): Iguchi, T.
  • Organizer: Mid-Continent Ecology Division
  • Place of Presentation: アメリカ環境保護庁(アメリカ、ミネソタ州)
• [Presentation] Effects of juvenile hormone agonists fro embryonic development of Daphnia magna (2010)
  • Author(s): Toyota K., Iguchi T., et al.
  • Organizer: 第13回環境ホルモン学会
  • Place of Presentation: 東京大学山上会館(東京)
  • Year and Date: 2010-12-16
• [Presentation] Sex determination mechanisms of water flea, Daphnia magna (2010)
  • Author(s): Iguchi T.
  • Organizer: Howard Bern Symposium
  • Place of Presentation: カリフォルニア大学バークレー校(アメリカ)
  • Year and Date: 2010-12-16
• [Presentation] オオミジンコの性分化機構 (2010)
  • Author(s): 井口泰泉
  • Organizer: 岡崎高校スーパーサイエンスハイスクール
  • Place of Presentation: 愛知県立岡崎高校(岡崎)
  • Year and Date: 2010-10-28
• [Presentation] Endocrine disruption in invertebrates : sex determination of Daphnia magna (2010)
  • Author(s): Iguchi, T
  • Organizer: 11^<th> e. hormone Symposium
  • Place of Presentation: アメリカ
  • Year and Date: 2010-10-19
• [Presentation] Endocrine disruption in invertebrates:sex determination of Daphnia magna11^<th> e.hormone Symposium (2010)
  • Author(s): Iguch T.
  • Organizer: 11^<th> e.hormone Symposium
  • Place of Presentation: チューレン大学(アメリカ)
  • Year and Date: 2010-10-19
• [Presentation] オオミジンコの性決定機構 (2010)
  • Author(s): 井口泰泉
  • Organizer: 日本動物学会第81回大会シンポジウム
  • Place of Presentation: 東京大学駒場キャンパス(東京)
  • Year and Date: 2010-09-23
• [Presentation] Effect of juvenile hormone agonists on neonatal Daphnia magna : Microarray analysis (2010)
  • Author(s): Toyota K., Iguchi T., et al.
  • Organizer: 第16回バイオアッセイ研究会日本環境毒性学会合同研究発表会
  • Place of Presentation: つくば国際会議場(つくば)
  • Year and Date: 2010-09-02
• [Book] Handbook of Systems Toxicology (2011)
  • Author(s): Watanabe, H., Kato, Y., Iguchi, T.
  • Publisher: John Wiley & Sons, Ltd
• [Remarks]
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html