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Development of reverse-genetics methods for Daphnids

Research Project

Project/Area Number 22651078
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied genomics
Research InstitutionNational Institutes of Natural Sciences Okazaki Research Facilities

Principal Investigator

IGUCHI Taisen  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90128588)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsミジンコ / 逆遺伝学 / エコゲノミクス
Research Abstract

The water flea Daphnia magna is a small crustacean which is an ubiquitous inhabitant in fresh water. As daphnids inhabit a diverse array of aquatic environments, they are a good model for understanding response and adaptation to environmental changes and evolution. Therefore, they have been used one of standard organisms in ecotoxicology. While daphnids have significant importance in ecotoxicology, tools to understand response to environmental changes in daphnia is very limited. We investigate a condition for natural male production and improve introduction of foreign DNA into daphnids. These basic information will be available for establishment of transgenic daphnids.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (35 results)

All 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 11 results) Presentation (19 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Environmental sex determination in the branchiopod crustacean Daphnia magna : Deep conservation of a Doublesex gene in the sex-determining pathway2011

    • Author(s)
      Kato, Y., Kobayashi, K., Watanabe, H. and Iguchi, T
    • Journal Title

      PLoS Genetics

      Volume: 7

    • NAID

      120004837638

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a RNA interference method in the cladoceran crustacean Daphnia magna2011

    • Author(s)
      Kato, Y., Shiga, Y., Kobayashi, K., Tokishita, S., Yamagata, H., Iguchi, T. and Watanabe, H
    • Journal Title

      Devel. Genes Evol

      Volume: 220 Pages: 337-345

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphological changes in Daphnia galeata induced by a crustacean terpenoid hormone and its analog2011

    • Author(s)
      Oda, S., Kato, Y., Watanabe, H., Tatarazako, N., Iguchi, T
    • Journal Title

      Environ Toxicol Chem

      Volume: 30 Pages: 232-238

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Demasculinization and feminization of male gonads by atrazine : consistent effects across vertebrate classes2011

    • Author(s)
      Hayes, T. B., Beasley, V. R., de Solla, S., Iguchi, T., et al
    • Journal Title

      J. Steroid Biochem Mol. Biol

      Volume: 127 Pages: 64-73

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endocrine disrupting chemicals. J. Steroid Biochem2011

    • Author(s)
      Blumberg, B., Iguchi, T. and Odermatt, A
    • Journal Title

      Mol. Biol

      Volume: 127 Pages: 1-3

    • Related Report
      2011 Final Research Report
  • [Journal Article] Species extrapolation for the 21st century. Environ2011

    • Author(s)
      Celander, M. C., Goldstone, J. V., Denslow, N. D., Iguchi, T., Kille, P., Meyerhoff, R. D., Smith, B. A., Hutchinson T. H. and Wheeler, J. R
    • Journal Title

      Toxicol. Chem

      Volume: 30 Pages: 5263-5263

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endocrine disrupting chemicals2011

    • Author(s)
      Blumberg, B., Iguchi, T., et al
    • Journal Title

      J Steroid Biochem Mol Biol

      Volume: 127 Pages: 1-3

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Demasculinization and feminization of male gonads by atrazine : consistent effects across vertebrate classes2011

    • Author(s)
      Hayes, T.B., Iguchi, T., et al
    • Journal Title

      J Steroid Biochem Mol Biol

      Volume: 127 Pages: 64-73

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Species extrapolation for the 21st century2011

    • Author(s)
      Celander, M.C, Iguchi, T., et al
    • Journal Title

      Environ Toxicol Chem

      Volume: 30 Pages: 52-63

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Environmental sex determination in the branchiopod crustacean Daphnia magna : Deep conservation of a Doublesex gene in the sex-determining pathway2011

    • Author(s)
      Kato Y., Iguchi T., et al.
    • Journal Title

      PLoS Genetics

      Volume: 7

    • NAID

      120004837638

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an RNA interference method in the cladoceran crustacean Daphnia magna.2011

    • Author(s)
      Kato Y., Iguchi T., et al.
    • Journal Title

      Devel.Genes Evol

      Volume: 220 Pages: 337-345

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequence divergence and expression of a transformer gene in the branchiopod crustacean2010

    • Author(s)
      Kato, Y., Kobayashi, K., Oda, S., Tatarazako, N., Watanabe, H. and Iguchi, T
    • Journal Title

      Daphnia magna. Genomics

      Volume: 95 Pages: 160-165

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation2010

    • Author(s)
      Kato, Y., Kobayashi, K., Watanabe, H. and Iguchi, T
    • Journal Title

      Ecotoxicology

      Volume: 19 Pages: 589-592

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological changes in Daphnia galeata induced by a crustacean terpenoid hormone and its analog2010

    • Author(s)
      Oda S., Iguchi T., et al.
    • Journal Title

      Environ.Toxicol.Chem

      Volume: 30 Pages: 232-238

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] オオミジンコの性分化機構2011

    • Author(s)
      井口泰泉
    • Organizer
      琉球大学熱帯生物圏研究センター共同利用研究会「動物の性決定機構」
    • Place of Presentation
      本部町産業支援センター(沖縄県本部町)
    • Year and Date
      2011-02-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 内分泌かく乱物質の生物影響に関する研究2011

    • Author(s)
      井口泰泉
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      北海道旭川市
    • Related Report
      2011 Final Research Report
  • [Presentation] Endocrine disrupters issues in Japan2011

    • Author(s)
      Iguchi, T
    • Organizer
      2nd International Conference on Endocrine Disruptors
    • Place of Presentation
      ドイツ
    • Related Report
      2011 Final Research Report
  • [Presentation] Endocrine disruption in invertebrates : Sex determination of Daphnia magna2011

    • Author(s)
      Iguchi, T
    • Organizer
      6th Copenhagen Workshop on Endocrine Disrupters
    • Place of Presentation
      デンマーク
    • Related Report
      2011 Final Research Report
  • [Presentation] オオミジンコのエクジクジステロイド生合成関連遺伝子群の単離及び発現解析2011

    • Author(s)
      角谷絵里, 井口泰泉, ら
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川市大雪クリスタルホール(北海道旭川市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] オオミジンコDaphnia magnaの性決定期における幼若ホルモン応答遺伝子の網羅的解析2011

    • Author(s)
      豊田賢治, 井口泰泉, ら
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川市大雪クリスタルホール(北海道旭川市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] ミジンコDaphnia pulexにおける捕食者カイロモンに応じた胚発生過程の変化2011

    • Author(s)
      宮川一志, 井口泰泉, ら
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川市大雪クリスタルホール(北海道旭川市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 内分泌かく乱物質の生物影響に関する研究2011

    • Author(s)
      井口泰泉, ら
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川市大雪クリスタルホール(北海道旭川市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of an RNA interference method in the cladoceran Daphnia magna2011

    • Author(s)
      Kato, Y., Iguchi, T., er al
    • Organizer
      分子生物学会第34回大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Endocrine disruption in invertebrates : Sex determination of Daphnia magna2011

    • Author(s)
      Iguchi, T.
    • Organizer
      6th Copenhagen Workshop on Endocrine Disrupters
    • Place of Presentation
      コペンハーゲン大学病院(デンマーク、コペンハーゲン)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Endocrine disrupters issues in Japan2011

    • Author(s)
      Iguchi, T., er al
    • Organizer
      2nd International Conference on Endocrine Disruptors
    • Place of Presentation
      ホリデーインフランクフルトエアポートノース(ドイツ、フランクフルト)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Estrogens and sex determination : Cross-species investigations with alligators, medaka, and Daphnia2011

    • Author(s)
      Iguchi, T.
    • Organizer
      Mid-Continent Ecology Division
    • Place of Presentation
      アメリカ環境保護庁(アメリカ、ミネソタ州)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effects of juvenile hormone agonists fro embryonic development of Daphnia magna2010

    • Author(s)
      Toyota K., Iguchi T., et al.
    • Organizer
      第13回環境ホルモン学会
    • Place of Presentation
      東京大学山上会館(東京)
    • Year and Date
      2010-12-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Sex determination mechanisms of water flea, Daphnia magna2010

    • Author(s)
      Iguchi T.
    • Organizer
      Howard Bern Symposium
    • Place of Presentation
      カリフォルニア大学バークレー校(アメリカ)
    • Year and Date
      2010-12-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] オオミジンコの性分化機構2010

    • Author(s)
      井口泰泉
    • Organizer
      岡崎高校スーパーサイエンスハイスクール
    • Place of Presentation
      愛知県立岡崎高校(岡崎)
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Endocrine disruption in invertebrates : sex determination of Daphnia magna2010

    • Author(s)
      Iguchi, T
    • Organizer
      11^<th> e. hormone Symposium
    • Place of Presentation
      アメリカ
    • Year and Date
      2010-10-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Endocrine disruption in invertebrates:sex determination of Daphnia magna11^<th> e.hormone Symposium2010

    • Author(s)
      Iguch T.
    • Organizer
      11^<th> e.hormone Symposium
    • Place of Presentation
      チューレン大学(アメリカ)
    • Year and Date
      2010-10-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] オオミジンコの性決定機構2010

    • Author(s)
      井口泰泉
    • Organizer
      日本動物学会第81回大会シンポジウム
    • Place of Presentation
      東京大学駒場キャンパス(東京)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effect of juvenile hormone agonists on neonatal Daphnia magna : Microarray analysis2010

    • Author(s)
      Toyota K., Iguchi T., et al.
    • Organizer
      第16回バイオアッセイ研究会日本環境毒性学会合同研究発表会
    • Place of Presentation
      つくば国際会議場(つくば)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Book] Handbook of Systems Toxicology2011

    • Author(s)
      Watanabe, H., Kato, Y., Iguchi, T.
    • Publisher
      John Wiley & Sons, Ltd
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/%7Ebioenv1/index-j.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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