Development of efficient cultivation method for nanobacteria isolated from human leukemia cell line.

• Project/Area Number: 22656188
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: University of Tsukuba
• Principal Investigator: AOYAGI Hideki 筑波大学, 生命環境系, 教授 (00251025)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,590,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: ナノバクテリア / 模擬微小重力培養 / ヒト血清 / 動物培養細胞 / 最適培養条件 / 微粒子結晶 / 血清 / 迅速検出 / 保存法 / 抗生物質 / 嫌気培養 / 微小重力 / 昆虫培養細胞 / 酸素供給

Research Abstract

Nanobacteria were originally isolated from the human leukemia cell line. After various investigations, the optimal component of the medium for culturing nanobacteria was determined. The nanobacteria could be cultivated at high density under low-shear modeled microgravity with optimal medium (this culture method could be used for detecting nanobacteria quickly). The various characteristics of nanobacteria used in this study were different from the nanobacteria-like crystal.

Research Products

• [Journal Article] Effects of low-shear modeled microgravity on a microbial community filtered through a 0.2-μm filter and its potential application in screening for novel microorganisms (2012)
  • Author(s): H. Aoyagi and A. Kuroda
  • Journal Title: Journal of Bioscience and Bioengineering Volume: (印刷中)(in press) Issue: 1 Pages: 73-79
  • DOI: 10.1016/j.jbiosc.2012.02.021
  • NAID: 110009479369
  • Peer Reviewed
• [Presentation] Effects of microgravity on microbial community and its application in screening for novel microorganisms (2011)
  • Author(s): H. Aoyagi and A. Kuroda
  • Organizer: 2nd World Congress on Biotechnology(招待講演)
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2011-11-29
• [Presentation] Effects of microgravity on microbial community and its application in screening for novel microorganisms (2011)
  • Author(s): A.Kuroda, H.Aoyagi
  • Organizer: 2nd World Congress on Biotechnology
  • Place of Presentation: Philadelphia, USA(招待講演)
  • Year and Date: 2011-11-29
• [Presentation] ヒト由来急性骨髄性白血病細胞系から分離したナノバクテリア様微粒子の特性(第6報) (2011)
  • Author(s): 青柳秀紀, ら
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京、東京農工大学
  • Year and Date: 2011-09-26
• [Presentation] ヒト由来急性骨髄性白血病細胞系から分離したナノバクテリア様微粒子の特性(第6報) (2011)
  • Author(s): 青柳秀紀, ら
  • Organizer: 第63回(平成23年度)日本生物工学会大会
  • Place of Presentation: 東京(東京農工大学)
  • Year and Date: 2011-09-26
• [Presentation] ヒト由来急性骨髄性白血病細胞系から分離したナノバクテリア様微粒子の特性(第5報) (2010)
  • Author(s): 青柳秀紀, ら
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎シーガイア
  • Year and Date: 2010-10-29
• [Presentation] ヒト由来急性骨髄性白血病細胞系から分離したナノバクテリア様微粒子の特性(第5報) (2010)
  • Author(s): 青柳秀紀, ら
  • Organizer: 第62回(平成22年度)日本生物工学会大会
  • Place of Presentation: 宮崎シーガイア
  • Year and Date: 2010-10-29