Analysis on cell filament formation and phenotypic plasticity of Escherichia coli using micro fluid system : rapid adaptation
| Project/Area Number |
22657006
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Ecology/Environment
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHIMADA Masakazu 東京大学, 大学院・総合文化研究科, 教授 (40178950)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Takehito 東京大学, 大学院・総合文化研究科, 准教授 (40447321)
WKAMOTO Yuichi 東京大学, 大学院・総合文化研究科, 准教授 (30517884)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,240,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | 1細胞培養計測系 / マイクロ流路 / 大腸菌 / 細胞伸長 / 表現型可塑性 / 捕食回避説 / 低密度良好説 / 環境ストレス説 / フィラメント形成 / 細菌の進化生態学 / 天敵の効果 / 栄養条件 |
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| Research Abstract |
Using the on-chip one cell micro fluid system, we analyzed three potential factors affecting the cell filament formation of Escherichia coli ;(1) the enemy avoidance hypothesis,(2) the cell performance enhanced at a low density, and(3) the environmental stress. Either one factor or their combinations has/have been analyzed. First of all, we developed the device, so that too many growing cells can be automatically removed from the incubating area(Patent 2011-156767). Applying the device to the Tetrahymena-E. coli co-incubation system, we observed that the cell filament of E. coli forms when we used for two standard mediums for Tetrahymena(1/10PYG and CDM), even though we did not add Tetrahymena's pheromones at all.
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Report
(3 results)
Research Products
(18 results)
