Expansion of the amino acid repertoire in protein biosynthesis of the domesticated silkworm, Bombyx mori
| Project/Area Number |
22658019
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
TERAMOTO Hidetoshi 独立行政法人農業生物資源研究所, 新機能素材研究開発ユニット, 主任研究員 (60391562)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,910,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | 昆虫 / カイコ / 蛋白質 / バイオテクノロジー / 非天然アミノ酸 |
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| Research Abstract |
Mutants of phenylalanyl-tRNA synthetase derived from the domesticated silkworm, Bombyx mori, recognizing phenylalanine(Phe) analogues(unnatural amino acids) as a substrate were generated by introducing point mutations into its amino acid binding pocket. The recognition capacity of the mutants toward several Phe analogues was elucidated in vitro, and the incorporation of Cl-Phe, one of Phe analogues, into protein biosynthesis in the cultured cells of B. mori overexpressing a PheRS mutant was verified. Moreover, a gene encoding methionyl-tRNA synthetase(MetRS) was cloned from B. mori and its complete cDNA sequence was clarified, and the recognition capacity of its mutants, generated by point mutation strategy similar to PheRS, toward methionine(Met) analogues was investigated in vitro.
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Report
(3 results)
Research Products
(10 results)
