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Investigation of Chaperone Function of Phospholipids Containing Polyunsaturated Fatty Acids and Their Application to Overproduction of Membrane Proteins

Research Project

Project/Area Number 22658028
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

KURIHARA Tatsuo  京都大学, 化学研究所, 教授 (70243087)

Co-Investigator(Renkei-kenkyūsha) KAWAMOTO Jun  京都大学, 化学研究所, 助教 (90511238)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords微生物 / 生体膜 / 高度不飽和脂肪酸 / エイコサペンタエン酸 / リン脂質 / 膜タンパク質 / フォールディング / ポーリン
Research Abstract

Shewanella livingstonensis Ac10 produces eicosapentaenoic acid-containing phospholipids (EPA-PLs) at low temperatures. We analyzed the role of EPA-PLs in the folding of Omp74, a major cold-inducible outer membrane protein of this bacterium. We found that the conformation of Omp74 in the mutant lacking EPA-PLs is different from that in the wild-type strain. In vitro experiments using liposomes showed that EPA-PLs function as a chemical chaperone in the membrane integration and folding of Omp74.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2013 2012 2011 2010

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (12 results)

  • [Journal Article] Eicosapentaenoic acid facilitates the folding of an outer membrane protein of the psychrotrophic bacterium2012

    • Author(s)
      Xian-Zhu Dai, Jun Kawamoto, Satoshi B. Sato, Nobuyoshi Esaki, and Tatsuo Kurihara
    • Journal Title

      Shewanella livingstonensis Ac10. Biochem. Biophys. Res. Commun.

      Volume: 425 Issue: 2 Pages: 363-7

    • DOI

      10.1016/j.bbrc.2012.07.097

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Occurrence of bacterial membrane microdomain at the cell division site enriched in phospholipids with polyunsaturated hydrocarbon chain.2012

    • Author(s)
      Sato, S., Kawamoto, J., Sato, S.B., Watanabe, B., Hiratake, J., Esaki, N., and Kurihara, T.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Issue: 29 Pages: 24113-21

    • DOI

      10.1074/jbc.m111.318311

    • NAID

      120005302081

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] dentification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis.2012

    • Author(s)
      Park, J., Kawamoto, J., Esaki, N., and Kurihara, T.
    • Journal Title

      Extremophiles

      Volume: 16 Issue: 2 Pages: 227-236

    • DOI

      10.1007/s00792-011-0422-z

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of 1-acyl-sn-glycerol-3-phosphate acyltransferase from a polyunsaturated fatty acid-producing bacterium2012

    • Author(s)
      Hyun-Nam Cho, Wataru Kasai, Jun Kawamoto, Nobuyoshi Esaki, and Tatsuo Kurihara
    • Journal Title

      Shewanella livingstonensis Ac10. Trace Nutrients Research

      Volume: 29 Pages: 92-9

    • URL

      http://www.jtnrs.com/sym29/19-No-P-14.pdf

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of an eicosapentaenoic acid biosynthesis protein Orf2 from a psychrotrophic bacterium2012

    • Author(s)
      Chunjie Gong, Jun Kawamoto, Nobuyoshi Esaki, and Tatsuo Kurihara
    • Journal Title

      Functional analysis of an eicosapentaenoic acid biosynthesis protein Orf2 from a psychrotrophic bacterium

      Volume: 29 Pages: 84-91

    • URL

      http://www.jtnrs.com/sym29/18-No-P-13.pdf

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Eicosapentaenoic acid plays a role in stabilizing dynamic membrane structure in the deep-sea piezophile Shewanella violacea2012

    • Author(s)
      Usui, Keiko, et al
    • Journal Title

      Biochim.Biophys.Acta

      Volume: 1818 Pages: 574-83

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro refolding of an OmpA homolog, a major cold-inducible outer membrane protein, from a psychrotrophic bacterium, Shewanella livingstonensis Ac102011

    • Author(s)
      Xian-Zhu Dai, Jun Kawamoto, NobuyoshiEsaki, and Tatsuo Kurihara
    • Journal Title

      Journal of Japanese Society for Extremophiles

      Volume: 10 Pages: 90-6

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures2011

    • Author(s)
      Kawamoto, J., Sato, T., Nakasone, K., Kato, C., Mihara, H., Esaki, N., and Kurihara, T
    • Journal Title

      Environmental Microbiology

      Volume: 13 Issue: 8 Pages: 2293-2298

    • DOI

      10.1111/j.1462-2920.2011.02487.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro refolding of an OmpA homolog, a major cold-inducible outer membrane protein, from a psychrotrophic bacterium, Shewanella livingstonensis Ac102011

    • Author(s)
      Dai, Xian-Zhu, et al
    • Journal Title

      Journal of Japanese Society for Extremophiles

      Volume: 10 Pages: 90-96

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violaceg DSS12, at high-hydrostatic pressures2011

    • Author(s)
      Kawamoto, Jun, et al
    • Journal Title

      Environ.Microbiol.

      Volume: 13 Pages: 2293-2298

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 化学的アプローチによる高度不飽和脂肪酸含有リン脂質の生理機能解析2013

    • Author(s)
      栗原達夫
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜市 パシフィコ横浜
    • Year and Date
      2013-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 細菌における高度不飽和脂肪酸含有リン脂質の生合成と機能2013

    • Author(s)
      栗原達夫
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台市 東北大学
    • Year and Date
      2013-03-27
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 好冷性細菌の低温適応を担う分子基盤:細胞膜における高度不飽和脂肪酸の機能2012

    • Author(s)
      栗原達夫
    • Organizer
      新化学技術推進協会講演会
    • Place of Presentation
      京都市 京都テルサ
    • Year and Date
      2012-11-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Shewanella livingstonensis Ac10 におけるエイコサペンタエン酸の機能2012

    • Author(s)
      栗原達夫
    • Organizer
      特殊環境微生物セミナー
    • Place of Presentation
      特殊環境微生物セミナー
    • Year and Date
      2012-11-22
    • Related Report
      2011 Final Research Report
  • [Presentation] Function of eicosapentaenoic acid-containing phospholipids in bacterial cell membrane2012

    • Author(s)
      栗原達夫
    • Organizer
      Hirschegg Summer School 2012
    • Place of Presentation
      Marburger Haus,Hirschegg, Austria
    • Year and Date
      2012-09-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 生体膜における高度不飽和脂肪酸含有リン脂質の機能2012

    • Author(s)
      栗原達夫
    • Organizer
      京都大学学際融合教育研究推進センター生理化学研究ユニット第2回シンポジウム
    • Place of Presentation
      京都市 京都大学
    • Year and Date
      2012-06-03
    • Related Report
      2011 Final Research Report
  • [Presentation] 細菌細胞膜における高度不飽和脂肪酸含有リン脂質の機能:局在化と膜タンパク質生合成における機能2012

    • Author(s)
      栗原達夫
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都市 京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 細菌細胞膜におけるリン脂質の局在化と生理機能2011

    • Author(s)
      栗原達夫
    • Organizer
      京都大学微生物科学寄附研究部門主催シンポジウム「微生物科学研究の現状と展望」
    • Place of Presentation
      京都市 芝蘭会館
    • Year and Date
      2011-06-23
    • Related Report
      2011 Final Research Report
  • [Presentation] 細菌細胞膜におけるリン脂質の局在化と生理機能2011

    • Author(s)
      栗原達夫
    • Organizer
      京都大学微生物科学寄附研究部門主催シンポジウム
    • Place of Presentation
      京都市・芝蘭会館(招待講演)
    • Year and Date
      2011-06-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Function of phospholipids containing eicosapentaenoic acid in the bacterial cell membrane2011

    • Author(s)
      栗原達夫
    • Organizer
      Wageningen University and Research Centre/Kyoto University Joint Workshop
    • Place of Presentation
      京都市 京都大学
    • Year and Date
      2011-04-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Molecular probes to analyze the dynamics of eicosapentaenoic-acid-containing phospholipids and their interaction with membrane proteins2011

    • Author(s)
      栗原達夫
    • Organizer
      第30回内藤コンファレンス
    • Place of Presentation
      札幌市 シャトレーゼ ガトーキングダムサッポロ
    • Related Report
      2011 Final Research Report
  • [Presentation] Construction of protein expression system by using cold-adapted bacteria as the host2010

    • Author(s)
      栗原達夫
    • Organizer
      MicroPerm Workshop
    • Place of Presentation
      Alfred Wegener Institute for Polar and Marine Research, Potsdam, Germany
    • Year and Date
      2010-11-08
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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