Establishment of novel reaction mechanism on glycosylase and its application
| Project/Area Number |
22658032
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
KIMURA Atsuo 北海道大学, 大学院・農学研究院, 教授 (90186312)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | 反応機構 / 糖質分解酵素 / 反応中間体 |
|---|
| Research Abstract |
Two reaction mechanisms have been proposed : on glycosylases, i. e. oxo-carbenium ion-mediated mechanism and covalent linkage-formation mechanism. Recently, the latter one is supported by many glycosylase-scientists. However, there is a problem in the latter mechanism : this mechanism cannot be supported by organic chemistry-related knowledge. A purpose of this project is an experimental proof of the oxo-carbenium ion-mediated mechanism. Glycosylases catalyze the useful reaction of transglycosylation. This reaction mechanism may produce the novel application approach, the possibility of which is also investigated.
|
Report
(3 results)
Research Products
(59 results)
-
[Journal Article] α-Glucosidase-catalyzed novel reaction on 1, 5-anhydrofructose, suggesting new metabolic pathway for production of glucose from starch2012
Author(s)
Kim YM, Saburi W, Yu S, Nakai H, Maneesan J, Kang MS, Chiba S, Kim D, Okuyama M, Mori H, Kimura A
-
Journal Title
J Biol Chem
Volume: (in press)
Related Report
Peer Reviewed
-
-
[Journal Article] Novel dextranase catalyzing cycloisomaltooligosaccharide-formation and its identification of catalytic amino acids and their functions using chemical rescue approach2012
Author(s)
Kim YM, Kiso Y, Muraki T, Kang MS, Nakai H, Saburi W, Lang W, Kang HK, Okuyama M, Mori H, Suzuki R, Funane K, Suzuki N, Momma M, Fujimoto Z, Oguma T, Kobayashi M, Kim D, Kimura A
-
Journal Title
J Biol Chem
Volume: (in press)
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities2010
Author(s)
Ryu HJ, Jin X, Lee JH, Woo HJ, Kim YM, Kim GJ, Seo ES, Kang HK, Kim J, Cho DL, Kimura A, Kim D
-
Journal Title
Enzyme Microb Technol
Volume: 47
Pages: 212-215
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Molecular mechanism of novel enzymes degrading and formingα-1, 6-glucosidic linkages, including megalosaccharide production2011
Author(s)
Kim YM, Kanegae M, Hondoh H, Nishimura T, Saburi W, Sadahiro J, Lang W, Shimizu R, Nakai H, Okuyama M, Mori H, Kang MS, Fujimoto Z, Funane K, Kim D, A Kimura A
Organizer
The 78th Annual Meeting and International Symposium in 2011 Annual Meeting of Korean Society of Food Science and Technology(KoSFoST)
Place of Presentation
Daegu Exhibition Convention Center(Daegu Metropolitan City, Kora)
Year and Date
2011-06-09
Related Report
-
-
[Presentation] Dietary influence of linearα-1, 6-polysaccharides on several parameters under experimental colitis depends on degree of the polymerization2011
Author(s)
Hitoshi Iwaya, Jae-Sung Lee, Shinya Yamagishi, Aki Shinoki, Weeranuch Lang, Hee-Kwon Kang, Hiroshi Hara, Atsuo Kimura, Satoshi Ishizuka
Place of Presentation
Royton Sapporo Hotel(Sapporo, Japan)
Related Report
-
-
-
-
-
-
-
[Presentation] Molecular anatomy ofα-1, 6-glucoside(s)-forming and-hydrolyzing enzymes, comprising megalosaccharide-producing enzymes2010
Author(s)
Young-Min Kim, Michiyo Kanegae, Hironori Hondoh, Takashi Nishimura, Wataru Saburi, Juri Sadahiro, Weeranuch Lang, Ryoko Shimizu, Hiroyuki Nakai, Hee-Kwon Kang, Masayuki Okuyama, Haruhide Mori, Min-Sun Kang, Zui Fujimoto, Kazumi Funane, Doman Kim, Kimura A
Organizer
2011 CAB Agricultural Biotechnology Symposium on "Agricultural Biomaterials for Next Generation"
Place of Presentation
Seoul Natinal University(Seoul, Korea)
Year and Date
2010-08-25
Related Report
-
-
-
-
-
-
-
-
