Evaluation of a hypothesis that "The intestinal detoxification system can be regulated by eating"

• Project/Area Number: 22658040
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: The University of Tokyo
• Principal Investigator: SHIMIZU Makoto 東京大学, 大学院・農学生命科学研究科, 教授 (30114507)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,650,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 食品 / 解毒代謝 / 腸管バリア / 解毒酵素系 / Caco-2細胞 / アリルハイドロカーボン受容体 / ダイオキシン / フラボノイド / 遺伝子発現変化 / 腸管

Research Abstract

Dioxin-induced expression of intestinal detoxification enzymes(DTE), such as CYPs, was suppressed by flavonoids. The CYP expression was also induced by certain flavonoids. DNA microarray analysis showed that dioxins and flavonoids caused different gene expression of intestinal DTE. Among eight flavonoids having the AhR agonistic activity, only one of them was able to induce regulatory T cell differentiation via AhR activation, suggesting that a small difference in the AhR/ligand interaction was responsible for the variability of gene expression.

Research Products

• [Journal Article] Dietary flavonoid naringenin induces regulatory T cells via an aryl hydrocarbon receptor(AhR)-mediated pathway (2012)
  • Author(s): Wang, H-K., Yeh, C-H., Iwamoto, T., Satsu, H., Shimizu, M., Totsuka, M.
  • Journal Title: J. Agric. Food Chem. Volume: 60(9) Pages: 2171-2178
• [Journal Article] Dietary Flavonoid Naringenin Induces Regulatory T Cells via an Aryl Hydrocarbon Receptor Mediated Pathway (2012)
  • Author(s): Hsi-Kai Wang
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 60 Pages: 2171-2178
  • Peer Reviewed
• [Journal Article] Metabolites of galangin by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P450 1A1 in human intestinal epithelial Caco-2 cells and their antagonistic activity toward aryl hydrocarbon receptor (2010)
  • Author(s): Hamada, M., Satsu, H., Ashida, H., Sugita-Konishi, Y., Shimizu, M.
  • Journal Title: J. Agric. Food Chem. Volume: 58 Pages: 8111-8118
• [Journal Article] Metabolites of galangin by 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P450 1A1 in human intestinal epithelial Caco-2 Cells and their antagonistic activity toward aryl hydrocarbon receptor. (2010)
  • Author(s): Hamada M., Satsu H., Ashida H,, Sugita-Konishi Y, Shimizu M.
  • Journal Title: J.Agric.Food Chem. Volume: 58 Pages: 8111-8118
  • Peer Reviewed
• [Presentation] 柑橘類由来ナリンゲニンにより誘導されるマウス制御性T細胞の機能解析 (2012)
  • Author(s): 王璽凱、葉鎮豪、岩本拓、薩秀夫、清水誠、戸塚護
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
• [Presentation] Screening and evaluation of dietary aryl hydrocarbon receptors (AhR) ligands inducing differentiation of regulatory T cells (2011)
  • Author(s): Wang, H-K.
  • Organizer: 5th International Conference on Functional Foods
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-11-22
• [Presentation] Modulation of intestinal barrier functions by food (2011)
  • Author(s): Shimizu, M.
  • Organizer: International Society for Nutraceuticals and Functional Foods (SNFF2011)
  • Place of Presentation: Sapporo, Japan(招待講演)
  • Year and Date: 2011-11-17
• [Presentation] Screening and evaluation of dietary arylhydrocarbon receptors(AhR) ligands inducing differentiation of regulatory T cells (2011)
  • Author(s): Wang, H-K., Iwamoto, T., Satsu, H., Shimizu, M., Totsuka, M
  • Organizer: 5th International Conference on Functional Foods
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Induction of detoxification enzymes by equol via activation of pregnane X receptor in human intestinal epithelial cells (2011)
  • Author(s): Satsu, H., Mizukami, T., Mikubo, A., Tachibana, N., Wanezaki, S., Kohno, M., Hamada, M., Iwayanagi, C., Ogiwara, H., Shimizu, M
  • Organizer: 5th International Conference on Functional Foods
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Naringeninと食品由来AHRリガンドによるマウス制御性T細胞の誘導機能 (2011)
  • Author(s): 王璽凱、岩本拓、薩秀夫、清水誠、戸塚護
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
• [Presentation] ブロッコリースプラウトがAhRに及ぼす影響 (2010)
  • Author(s): 三久保絢之、薩秀夫、水上朋彦、濱田美影、吉田和敬、牛田悠介、森啓信、稲熊隆博、清水誠
  • Organizer: 日本農芸化学会関東支部2010年度大会
  • Place of Presentation: 千葉
  • Year and Date: 2010-10-09
• [Book] 食の安全科学の展開-食のリスク予測と制御に向けて(分担執筆=腸管の解毒機能と食の安全) (2010)
  • Author(s): 清水誠、薩秀夫(東京大学・食の安全研究センター編)
  • Total Pages: 287
  • Publisher: シーエムシー出版