| Project/Area Number |
22658085
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Azabu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Junya 麻布大学, 獣医学部, 准教授 (30454143)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | 哺乳類 / 卵 / 凍結保存 |
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| Research Abstract |
The aim of the present study was to improve vitrification of mouse oocytes. We examined whether carboxyrated e-poly-L-lysine(COOH-PLL), a new cryoprotective agent, is available for vitrification of mouse embryos. When the oocytes were vitrified with 20%(v/v) ethylene glycol and 10%(w/v) COOH-PLL, the fertility and developmental ability were significantly higher than those in other groups. These embryos at the 2 cell stage were transferred to recipient females. Most of the recipients became pregnant and pups were delivered. In conclusion, our data clearly showed COOH-PLL is a useful cryoprotective agent for vitrification of mouse oocytes. These findings will contribute to improve oocyte vitrification in not only experimental animals but also clinical application for human infertility.
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