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A novel signal transduction pathway which regulates the structure of P-body and the dynamics of ARE-mRNAs

Research Project

Project/Area Number 22659015
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

KATADA Toshiaki  東京大学, 大学院・薬学系研究科, 教授 (10088859)

Co-Investigator(Kenkyū-buntansha) KAJIHO Hiroaki  東京大学, 大学院・薬学系研究科, 助教 (70401221)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsシグナル伝達 / G蛋白質 / P-body / mRNA動態 / Gサイクル / ゲノムプロジェクト / Processing body
Research Abstract

Processing bodies(P-bodies) contain a common set of conserved RNA-processing enzymes, and mRNAs with AU-rich elements(AREs) are delivered to P-bodies for translational silencing. It is unclear how small GTPases are involved in the P-body regulation and the ARE-mRNA metabolism. We found here that overexpression of the RhoA-subfamily GTPases alters the P-body dynamics in HeLa cells. Interestingly, both RhoA activation and glucose depletion inhibit the mRNA accumulation and degradation. These findings indicate that RhoA participates in the stress-induced rearrangement of P-bodies and the release of nucleated ARE-mRNAs for their stabilization.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (28 results)

All 2012 2011 2010 Other

All Journal Article (16 results) (of which Peer Reviewed: 14 results) Presentation (6 results) Book (4 results) Remarks (2 results)

  • [Journal Article] RINL, guanine nucleotide exchange factor Rab5-subfamily, is involved in the EphA8-degradation pathway with odin2012

    • Author(s)
      Kajiho H, Fukushima S, Kontani K, Katada T.
    • Journal Title

      Plo S One

      Volume: 7(1)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel acetylation cycle of the transcription co-activator Yes-associated protein that is downstream of the Hippo pathway is triggered in response to SN2 alkylating agents2012

    • Author(s)
      Hata S, Hirayama J, Kajiho H, Nakagawa K, Hata Y, Katada T, Furutani-Seiki M, Nishina H.
    • Journal Title

      J. Biol. Chem

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex2011

    • Author(s)
      Yamasaki T, Kawasaki H, Arakawa S, Shimizu K, Shimizu S, Reiner O, Okano H, Nishina S, Azuma N, Penninger JM, Katada T, Nishina H.
    • Journal Title

      J Neurosci

      Volume: 31 Pages: 16872-16883

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of RIN3 as a guanine-nucleotide exchange factor for the Rab5-subfamily GTPase Rab312011

    • Author(s)
      Kajiho H, Sakurai, K, Minoda T, Yoshikawa M, Nakagawa S, Fukushima S, Kontani K, Katada T.
    • Journal Title

      J. Biol. Chem

      Volume: 286 Pages: 24364-24373

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] cTAGE5 mediates collagen secretion through interaction with TANGO1 at endoplasmic reticulum exit sites2011

    • Author(s)
      Saito K, Yamashiro K, Ichikawa Y, Erlmann P, Kontani K, Malhotra V, Katada T.
    • Journal Title

      Mol. Biol. Cell

      Volume: 22 Pages: 2301-2308

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] RASSF7 negatively regulates pro-apoptotic JNK signaling by inhibiting the activity of hosphorylated-MKK72011

    • Author(s)
      Takahashi S, Ebihara A, Kajiho H, Kontani K, Nishina H, Katada T.
    • Journal Title

      Cell Death Differ

      Volume: 18 Pages: 645-655

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs2011

    • Author(s)
      Takahashi S, Sakurai K, Ebihara A, Kajiho H, Saito K, Kontani K, Nishina H, Katada T.
    • Journal Title

      Nucleic Acids Res

      Volume: 39 Pages: 3446-3457

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs2011

    • Author(s)
      Takahashi, S., Sakurai, K., Ebihara, A., Kajiho, H., Saito, K., Kontani, K., Nishina, H., and Katada, T.
    • Journal Title

      Nucleic Acids Res

      Volume: 39 Issue: 8 Pages: 3446-3457

    • DOI

      10.1093/nar/gkq1302

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RASSF7 negatively regulates pro-apoptotic JNK signaling by inhibiting the activity of phosphorylated-MKK72011

    • Author(s)
      Takahashi, S., Ebihara, A., Kajiho, H., Kontani, K., Nishina, H., and Katada, T.
    • Journal Title

      Cell Death Differ

      Volume: 18 Issue: 4 Pages: 645-655

    • DOI

      10.1038/cdd.2010.137

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAS.2011

    • Author(s)
      Takahashi s, et. al.
    • Journal Title

      Nucleic Acids Res.

      Volume: 39(in press)(掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Arf-like GTPase Arl8 mediates delivery of ndocytosed macromolecules to lysosomes in Caenorhabditis elegans2010

    • Author(s)
      Nakae I, Fujino T, Kobayashi T, Sasaki A, Kikko Y, Fukuyama M, Gengyo-Ando K, Mitani S, Kontani K, Katada T.
    • Journal Title

      Mol. Biol. Cell

      Volume: 21 Pages: 2434-2442

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans2010

    • Author(s)
      Cevik S, Hori Y, Kaplan OI, Kida K, Toivenon T, Foley-Fisher C, Cottell D, Katada T, Kontani K, Blacque OE.
    • Journal Title

      J. Cell Biol

      Volume: 188 Pages: 953-969

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative characterization of Tob interactions provides the thermodynamic basis for translation termination-coupled deadenylase regulation2010

    • Author(s)
      Ruan L, Osawa M, Hosoda N, Imai S, Machiyama A, Katada T, Hoshino S, Shimada I.
    • Journal Title

      J. Biol. Chem

      Volume: 285 Pages: 27624-27631

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] AnArf-like small G protein, ARL-8, promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation2010

    • Author(s)
      Klassen MP, Wu YE, Maeder CI, Nakae I, Cueva JG, Lehrman EK, Tada M, Gengyo-Ando K, Wang GJ, Goodman M, Mitani S, Kontani K, Katada T, Shen K.
    • Journal Title

      Neuron

      Volume: 66 Pages: 710-723

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gタンパク質研究の動向2010

    • Author(s)
      堅田利明
    • Journal Title

      細胞(The Cell)

      Volume: 42(3) Pages: 90-91

    • Related Report
      2011 Final Research Report
  • [Journal Article] アミノ酸シグナル伝達に介在するGタンパク質Rag2010

    • Author(s)
      春日秀文、福山征光、紺谷圏二、堅田利明
    • Journal Title

      細胞(The Cell)

      Volume: 42(3) Pages: 108-111

    • Related Report
      2011 Final Research Report
  • [Presentation] 分子スイッチとしてのGTP結合蛋白質(特別講演)2012

    • Author(s)
      堅田利明
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-02-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 百日咳毒素の作用機構からG蛋白質を見出した背景.2011

    • Author(s)
      堅田利明
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞のシグナル伝達系に介在するG蛋白質2011

    • Author(s)
      堅田利明
    • Organizer
      第77回日本生化学会東北支部例会
    • Place of Presentation
      仙台
    • Year and Date
      2011-07-23
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞のシグナル伝達系に介在するGタンパク質: Giの発見から、Gタンパク質が果たす役割の拡大に向けて2010

    • Author(s)
      堅田利明
    • Organizer
      特別講義
    • Place of Presentation
      東京医科歯科大学
    • Year and Date
      2010-10-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞のシグナル伝達系に介在するGTP結合性制御蛋白質(G蛋白質)2010

    • Author(s)
      堅田利明
    • Organizer
      特別講義
    • Place of Presentation
      大阪大学・医学部
    • Year and Date
      2010-05-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 低分子量G蛋白質Rab5GEF RINファミリーによる小胞輸送の機構解析2010

    • Author(s)
      梶保博昭、池田誠一、福島槙一、堅田利明
    • Organizer
      第1回GPCM
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Book] John W. Pelly生化学(インテグレーテッドシリーズ1)共訳2010

    • Author(s)
      堅田利明、金保安則
    • Publisher
      東京化学同人
    • Related Report
      2011 Final Research Report
  • [Book] 細胞内情報伝達;スタンダード薬学シリーズ4生物系薬学II.細胞をミクロに理解する第2版(日本薬学会編)2010

    • Author(s)
      堅田利明
    • Publisher
      東京化学同人
    • Related Report
      2011 Final Research Report
  • [Book] 分子細胞生物学第6版(翻訳分担; 15、16章)2010

    • Author(s)
      堅田利明
    • Publisher
      東京化学同人
    • Related Report
      2011 Final Research Report
  • [Book] ストライヤー基礎生化学第1版(翻訳分担; 12、15、16章)2010

    • Author(s)
      堅田利明
    • Publisher
      東京化学同人
    • Related Report
      2011 Final Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.f.u-tokyo.ac.jp/~seiri

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.f.u-tokyo.ac.jp/~seiri

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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